Tissue expression pattern of ABCG transporter indicates functional roles in reproduction of Toxocara canis

Very long-chain (˃ C24) fatty acids (VLC-FA) play critical roles during early development of vertebrates, since these compounds are accumulated in the rapidly forming neural tissues, ensuring their normal function. The functionality of VLC-FA has aroused scientific interest, focusing on the study of elongases protein 4 of very long chain fatty acid (Elovl4), which are responsible for their biosynthesis from shorter fatty acids (precursors). For a better understanding of the metabolism and the potential tissue-specific requirements of VLC-FA in marine teleosts, the present study aimed to determine the tissue-expression pattern of the genes that encode for Elovl4 isoforms, i.e. elovl4a and elovl4b, in different windows of development (larval and adult stages) of Gilthead seabream Sparus aurata and Senegalese sole Solea senegalensis. The results indicated that in S. aurata larvae, elovl4a is widely expressed in the head, while elovl4b is strongly focused in the eyes. Interestingly, in S. senegalensis larvae an opposite tissue-expression pattern was observed for both elovl4 isoforms. In adults of both fish, although elovl4 transcripts were detected in most tissues analyzed, elovl4a and elovl4b genes were strongly expressed in brain and eyes, respectively. Importantly, the differential tissue-expression pattern of both elovl4 isoforms associated to the pre- and post-metamorphic stage of S. senegalensis could be indicative of the VLC-FA particular needs linked to neural tissues functionality in each development stage. These findings can contribute to a better understanding of the species-specific VLC-FA metabolism in marine teleosts.

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Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake

Genetics and Molecular Research ◽

10.4238/gmr.15037998 ◽

2016 ◽

Vol 15 (3) ◽

Cited By ~ 2

Author(s):

L. Pu ◽

L.C. Zhang ◽

J.S. Zhang ◽

X. Song ◽

L.G. Wang ◽

...

Keyword(s):

Molecular Cloning ◽

Expression Pattern ◽

Feed Intake ◽

Copy Number ◽

Residual Feed Intake ◽

Tissue Expression ◽

Copy Number Variations ◽

Tissue Expression Pattern

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Expression Analysis of the Prolific Candidate Genes, BMPR1B, BMP15, and GDF9 in Small Tail Han Ewes with Three Fecundity (FecB Gene) Genotypes

Animals ◽

10.3390/ani8100166 ◽

2018 ◽

Vol 8 (10) ◽

pp. 166 ◽

Cited By ~ 11

Author(s):

Jishun Tang ◽

Wenping Hu ◽

Ran Di ◽

Qiuyue Liu ◽

Xiangyu Wang ◽

...

Keyword(s):

Gene Expression ◽

Candidate Genes ◽

Expression Pattern ◽

Expression Analysis ◽

Regulatory Mechanism ◽

Follicular Development ◽

Tissue Expression ◽

Rt Pcr ◽

Tissue Expression Pattern ◽

Systematic Analysis

The expression characteristics of the prolific candidate genes, BMPR1B, BMP15, and GDF9, in the major visceral organs and hypothalamic–pituitary–gonadal (HPG) axis tissues of three FecB genotypes (FecB BB, FecB B+, and FecB ++) were explored in STH ewes using RT-PCR and qPCR. The results were as follows, BMPR1B was expressed in all FecB BB genotype (Han BB) tissues, and GDF9 was expressed in all selected tissues, but BMP15 was specifically expressed in the ovaries. Further study of ovarian expression indicated that there was no difference in BMPR1B expression between genotypes, but the FecB B+ genotype (Han B+) had greater expression of GDF9 and BMP15 than Han BB and FecB ++ genotype (Han ++) (p < 0.05, p < 0.01). BMP15 expression was lower in the ovaries of Han BB than in Han ++ sheep, but the reverse was shown for GDF9. The gene expression in non-ovarian tissues was also different between genotypes. Therefore, we consider that the three genes have an important function in ovine follicular development and maturation. This is the first systematic analysis of the tissue expression pattern of BMPR1B, BMP15, and GDF9 genes in STH sheep of the three FecB genotypes. These results contribute to the understanding of the molecular regulatory mechanism for ovine reproduction.

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