scholarly journals Patrón de expresión tisular de los genes elovl4 en Sparus aurata y Solea senegalensis: de larva a adulto

2021 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Miguel Torres-Rodríguez ◽  
Francisco Hontoria Danés ◽  
Óscar Monroig Marzá ◽  
Inmaculada Varó Vaello ◽  
Juan Carlos Navarro Tárrega
Keyword(s):  
Fatty Acids ◽  
Expression Pattern ◽  
Sparus Aurata ◽  
Tissue Expression ◽  
Development Stage ◽  
Solea Senegalensis ◽  
Gilthead Seabream ◽  
Tissue Expression Pattern ◽  
Neural Tissues ◽  
Long Chain

Very long-chain (˃ C24) fatty acids (VLC-FA) play critical roles during early development of vertebrates, since these compounds are accumulated in the rapidly forming neural tissues, ensuring their normal function. The functionality of VLC-FA has aroused scientific interest, focusing on the study of elongases protein 4 of very long chain fatty acid (Elovl4), which are responsible for their biosynthesis from shorter fatty acids (precursors). For a better understanding of the metabolism and the potential tissue-specific requirements of VLC-FA in marine teleosts, the present study aimed to determine the tissue-expression pattern of the genes that encode for Elovl4 isoforms, i.e. elovl4a and elovl4b, in different windows of development (larval and adult stages) of Gilthead seabream Sparus aurata and Senegalese sole Solea senegalensis. The results indicated that in S. aurata larvae, elovl4a is widely expressed in the head, while elovl4b is strongly focused in the eyes. Interestingly, in S. senegalensis larvae an opposite tissue-expression pattern was observed for both elovl4 isoforms. In adults of both fish, although elovl4 transcripts were detected in most tissues analyzed, elovl4a and elovl4b genes were strongly expressed in brain and eyes, respectively. Importantly, the differential tissue-expression pattern of both elovl4 isoforms associated to the pre- and post-metamorphic stage of S. senegalensis could be indicative of the VLC-FA particular needs linked to neural tissues functionality in each development stage. These findings can contribute to a better understanding of the species-specific VLC-FA metabolism in marine teleosts.  

scholarly journals Cloning, tissue expression pattern and daily rhythms of Period1, Period2, and Clock transcripts in the flatfish Senegalese sole, Solea senegalensis

2012 ◽  
Vol 182 (5) ◽  
pp. 673-685 ◽  
Author(s):  
Águeda J. Martín-Robles ◽  
David Whitmore ◽  
Francisco Javier Sánchez-Vázquez ◽  
Carlos Pendón ◽  
José A. Muñoz-Cueto
Keyword(s):  
Expression Pattern ◽  
Tissue Expression ◽  
Solea Senegalensis ◽  
Senegalese Sole ◽  
Daily Rhythms ◽  
Tissue Expression Pattern

scholarly journals Molecular and Functional Characterization of Elovl4 Genes in Sparus aurata and Solea senegalensis Pointing to a Critical Role in Very Long-Chain (>C24) Fatty Acid Synthesis during Early Neural Development of Fish

2020 ◽  
Vol 21 (10) ◽  
pp. 3514 ◽  
Author(s):  
Sofia Morais ◽  
Miguel Torres ◽  
Francisco Hontoria ◽  
Óscar Monroig ◽  
Inma Varó ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Neural Development ◽  
Sparus Aurata ◽  
Critical Role ◽  
Functional Characterization ◽  
Acid Synthesis ◽  
Solea Senegalensis ◽  
Preferential Expression ◽  
Neural Tissues ◽  
Long Chain

Very long-chain fatty acids (VLC-FA) play critical roles in neural tissues during the early development of vertebrates. However, studies on VLC-FA in fish are scarce. The biosynthesis of VLC-FA is mediated by elongation of very long-chain fatty acid 4 (Elovl4) proteins and, consequently, the complement and activity of these enzymes determines the capacity that a given species has for satisfying its physiological demands, in particular for the correct development of neurophysiological functions. The present study aimed to characterize and localize the expression of elovl4 genes from Sparus aurata and Solea senegalensis, as well as to determine the function of their encoded proteins. The results confirmed that both fish possess two distinct elovl4 genes, named elovl4a and elovl4b. Functional assays demonstrated that both Elovl4 isoforms had the capability to elongate long-chain (C20–24), both saturated (SFA) and polyunsaturated (PUFA), fatty acid precursors to VLC-FA. In spite of their overlapping activity, Elovl4a was more active in VLC-SFA elongation, while Elovl4b had a preponderant elongation activity towards n-3 PUFA substrates, particularly in S. aurata, being additionally the only isoform that is capable of elongating docosahexaenoic acid (DHA). A preferential expression of elovl4 genes was measured in neural tissues, being elovl4a and elovl4b mRNAs mostly found in brain and eyes, respectively.

scholarly journals Towards a deeper understanding of fatty acid bioaccessibility and its dependence on culinary treatment and lipid class: a case study of gilthead seabream (Sparus aurata)

2016 ◽  
Vol 116 (10) ◽  
pp. 1816-1823 ◽  
Author(s):  
Sara Costa ◽  
Cláudia Afonso ◽  
Carlos Cardoso ◽  
Rui Oliveira ◽  
Francisca Alves ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Class ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Total Lipids ◽  
Class A ◽  
The Impact ◽  
Long Chain

AbstractThe bioaccessibility of total lipids and fatty acids (FA) in raw and grilled gilthead seabream (Sparus aurata) was determined using an in vitro digestion model. The particular impact of grilling on the FA profile of seabream was also studied. In addition, the influence of lipid class on the bioaccessibility of each FA was analysed. Grilling did not change the relative FA profile, and only the absolute values were altered. However, the relative FA profile varied across lipid classes, being more dissimilar between TAG and phospholipids. Long-chain SFA and PUFA seemed to be less bioaccessible. Moreover, grilling reduced bioaccessibility of protein, fat and many FA, with the highest reductions found in PUFA such as the DHA. Strong evidence supporting a predominantly regioselective action of lipase during in vitro digestion was found, and the impact of this phenomenon on FA bioaccessibility was assessed.

Identification of a β1 integrin isoform with restricted tissue expression in a teleost fish

2011 ◽  
Vol 23 (5) ◽  
pp. 654 ◽  
Author(s):  
Patricia Castillo-Briceño ◽  
Isabel Cabas ◽  
Marta Arizcun ◽  
Jose Meseguer ◽  
Victoriano Mulero ◽  
...  
Keyword(s):  
Teleost Fish ◽  
Sparus Aurata ◽  
Expression Profiles ◽  
Specific Inhibitor ◽  
Serum Levels ◽  
Tissue Expression ◽  
Gilthead Seabream ◽  
Β1 Integrin ◽  
Dependent Manner ◽  
Tissue Remodelling

The composition and organisation of extracellular matrix (ECM)-related molecules change during development. These components interact with different cell surface receptors to modulate the transduction of signals for cell growth, differentiation, migration, proliferation and apoptosis. Previous findings in the teleost fish gilthead seabream (Sparus aurata L., Teleostei), a marine protandrous hermaphrodite fish, showed that endocrine and immune stimuli are able to modulate the expression of ECM-related molecules, as well as specific correlations between them. In the present study, quantitative reverse transcription–polymerase chain reaction was used to examine the gene expression profile of β1 integrin isoform b (ITGB1b) and its possible role in reproductive physiology, especially in relation to spermatogenesis. Expression profiles were analysed in the context of the reproductive cycle (RC) and in relation with other ECM-related molecules, including matrix metalloproteinase (MMP)-2, MMP-9, MMP-13, tissue-specific inhibitor of metalloproteinase (TIMP)-2a, TIMP-2b, collagen (COL1A1) and ITGB1a. Expression of ITGB1b was found in the testis and brain and, to some extent, in endothelial cells. In contrast, ITGB1a was expressed ubiquitously. In the testis, the ITGB1b expression peaked during spermatogenesis, whereas the expression of the other ECM-related molecules is induced mainly during the post-spawning stage, both stages of marked tissue remodelling during the first and second RC in males. In addition, in fish exposed to the endocrine disruptor 17α-ethynyloestradiol (at 5 and 50 μg g–1 food during 7, 14 and 21 days), ITGB1b expression in the testis was inhibited in a dose- and time-dependent manner and was related to reduced serum levels of testosterone. Together, these results suggest a different functionality for the two ITGB1 isoforms in the gilthead seabream, where ITGB1b is more specifically involved in reproduction. This is the first report of an ITGB1 gene isoform whose expression is restricted to endocrine-related tissues in vertebrates.

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