Genomic analysis of cultivated barley (Hordeum vulgare) using sequence-tagged molecular markers. Estimates of divergence based on RFLP and PCR markers derived from stress-responsive genes, and simple-sequence repeats (SSRs)

2002 ◽  
Vol 267 (2) ◽  
pp. 186-201 ◽  
Author(s):  
E. Maestri ◽  
A. Malcevschi ◽  
A. Massari ◽  
N. Marmiroli
Keyword(s):  
Molecular Markers ◽  
Hordeum Vulgare ◽  
Simple Sequence Repeats ◽  
Genomic Analysis ◽  
Pcr Markers ◽  
Cultivated Barley ◽  
Stress Responsive Genes ◽  
Simple Sequence

scholarly journals ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

2009 ◽  
Vol 37 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Salima Machkour‐M'Rabet ◽  
Yann Hénaut ◽  
Ariane Dor ◽  
Gabriela Pérez-Lachaud ◽  
Céline Pélissier ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Detection of Genetic Polymorphism in Seven Barley Hordeum vulgare L. Varieties Using SSR

2020 ◽  
Vol 33 (2) ◽  
pp. 115-124
Author(s):  
Maha R. Khalil ◽  
Hussain A. Almahasneh ◽  
Salam Y. Lawand
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Polymorphism ◽  
Hordeum Vulgare ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Hordeum Vulgare L ◽  
Simple Sequence

Investigation was carried out at the laboratory of Biotechnology, Faculty of Agriculture, Damascus University, during the season 2017-2018. Seven varieties were planted to determine the degree of genetic similarity using SSR-technique (Simple Sequence Repeats), and 14 double primers were used for this purpose. The analysis results revealed that all primers showed polymorphism among the evaluated varieties, except Bmag0385. primers produced a total of 42 alleles with a polymorphic percentage of 88.27%. The number of alleles for each primer varied from 1 allele for the primer (Bmac0067) to 7 alleles for the primer (Bmag0006) in average of 3 alleles per primer. Cluster analysis and Dendrogram showed the highest degree of genetic similarity between variety Arabi asuad  and variety Arabi abiad (0.7619). While it was low between variety Fourat4 and variety Arabi abiad (0.3571), and varieties Fourat4 and Fourat3 (0.3571) which indicated wide genetic diversity among them.

Leaf scald resistance genes in Hordeum vulgare and Hordeum vulgare ssp. spontaneum: parallels between cultivated and wild barley

2003 ◽  
Vol 54 (12) ◽  
pp. 1335 ◽  
Author(s):  
R. K. Genger ◽  
K. J. Williams ◽  
H. Raman ◽  
B. J. Read ◽  
H. Wallwork ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Hordeum Vulgare ◽  
Wild Barley ◽  
Infection Type ◽  
Field Trials ◽  
Field Resistance ◽  
Adult Plant ◽  
Sources Of Resistance ◽  
Ssr Loci ◽  
Cultivated Barley

The prerequisite for breeding barley varieties with durable scald resistance is a diversity of genes each with molecular markers for their manipulation in crosses. Here we compare the outcomes of genetic analysis of scald resistance in 5 doubled haploid mapping populations of cultivated barley with 9 third-backcross families that derive from a set of diverse wild barley populations. In all cases, resistance was assessed as seedling infection type responses, but the Sloop/Halcyon population was also tested for adult plant resistance in field trials at two sites. In the latter case, the major quantitative trait locus for field resistance was coincident with that for seedling response on chromosome 3H. Most of the other cultivated barley sources of resistance in this study (1 cultivar and 3 breeders populations synthesised for resistance) also resolved to markers in the region of this 3H locus. In contrast, the genetic basis of resistance in wild barley populations resolved to at least 5 chromosome locations (1HS, 3H, 6HS, 7HL, and 7HS). Useful markers were of several kinds including proteins, isozymes, PCR based on RFLPs, AFLP, and SSR loci. Thus, wild barley increases the range of options for pyramid breeding and provides linked molecular markers that will be useful in manipulating those genes, or in the analysis of resistance in new sources.

scholarly journals Identification of Molecular Markers for Resistance to Pre-Harvest Sprouting in Triticum aestivum

2013 ◽  
Vol 70 (1) ◽  
pp. 283-286
Author(s):  
Eszter VAS ◽  
Constantin BOTEZ ◽  
Ioan HAȘ ◽  
Marion Rá–DER
Keyword(s):  
Triticum Aestivum ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Grain Quality ◽  
High Humidity ◽  
Polymorphic Markers ◽  
Parental Lines ◽  
The Cross ◽  
Sprouting Resistance ◽  
Simple Sequence

Pre-harvest sprouting (PHS) represents a worldwide major problem in wheat production, because it causes huge loss of grain quality. PHS occurs when germination of grains takes place in the spike before harvest and it is influenced by high humidity. The goal of this study was to find QTLs (quantitative trait loci) on chromosomal regions from Romanian wheat and the identification of specific markers that can be used for plant breeding in order to improve resistance to pre-harvest sprouting. The parental lines with different sensibility to pre-harvest sprouting (Turda 95-sensible, Turda 18-94-resistant, Lovrin 32-sensible, Fundulea 29 - resistant) were used for the genotypic characterization using microsatellite markers. A total of 119 from 640 SSR (simple sequence repeats) markers used on the parental lines indicated polymorphism. The initial primer test with the polymorphic markers on the cross lines: Turda 95(S) x Turda 18-94(R), Turda 95(S) x Fundulea 29 (R), Turda 18-94(R) x Lovrin 32 (S) showed that the cross between Turda 95(S) x Turda 18-94(R) had the highest number of segregating markers in the population. 111 F2 lines of the cross Turda 95(S) x Turda 18-94(R) now are being genotypic and phenotypic characterized in order to detect the QTLs underlying pre-harvest sprouting resistance.

scholarly journals Analysis of Diversity in Chinese Cultivated Barley with Simple Sequence Repeats: Differences Between Eco-Geographic Populations

2009 ◽  
Vol 48 (1-2) ◽  
pp. 44-56 ◽  
Author(s):  
Feixue Chen ◽  
Defu Chen ◽  
María-Pilar Vallés ◽  
Zhen Gao ◽  
Xiwen Chen
Keyword(s):  
Simple Sequence Repeats ◽  
Geographic Populations ◽  
Cultivated Barley ◽  
Simple Sequence

Genetic diversity and molecular markers of five snapper species

2007 ◽  
Vol 4 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Liu Li ◽  
Liu Chu-Wu
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Microsatellite Loci ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
The Other ◽  
Information Contents ◽  
The Mean ◽  
Simple Sequence

AbstractIn order to protect and develop valuable snappers (Lutjanus spp.), genetic diversity and molecular markers of five species (Lutjanus vitta, L. fulvus, L. fulviflamma, L. sebae and L. stellatus) were detected and analysed using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) techniques. The polymorphic loci ratio (P) (86.00–92.11%), the mean intraspecies genetic distances (D) (0.1775–0.3431) and the intraspecies genetic diversity indexes (Hi) (0.1022–0.1634) were calculated using the RAPD technique. The genetic diversities of L. fulviflamma and L. vitta were richest in terms of P, and D and Hi, respectively. The results of SSR showed that low effective numbers of alleles (1.7893–3.6591), medium average heterozygosities (0.332–0.676) and medium polymorphism information contents (PIC) (0.302–0.641) occurred in five species of snappers, indicating comparatively rich genetic diversity among these fish. Nine molecular markers in the products amplified by primers OPA8 and OPP10, and six molecular markers in 11 microsatellite loci were found to be useful as specific markers to identify five species of snappers. Two neighbour-joining (NJ) dendrograms based on the results of RAPD and SSR suggested that L. stellatus and L. sebae are closely related and clustered in one branch, with L. vitta, L. fulviflamma and L. fulvus in the other.

scholarly journals Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

2017 ◽  
Vol 52 (10) ◽  
pp. 849-860 ◽  
Author(s):  
Kenia Gracielle da Fonseca ◽  
Fábio Gelape Faleiro ◽  
Nilton Tadeu Vilela Junqueira ◽  
Mariana Barth ◽  
Nelson Pires Feldberg
Keyword(s):  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Multivariate Analyses ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Passion Fruit ◽  
Validation Rate ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.

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