Coordinate regulation of gene expression in the C. elegans excretory cell by the POU domain protein CEH-6

2009 ◽  
Vol 283 (1) ◽  
Author(s):  
Kristin R. Armstrong ◽  
Helen M. Chamberlin
Keyword(s):  
Gene Expression ◽  
Regulation Of Gene Expression ◽  
Coordinate Regulation ◽  
C Elegans ◽  
Pou Domain ◽  
Domain Protein ◽  
Excretory Cell

scholarly journals Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3 HDAC complex

2018 ◽  
Author(s):  
F. Beurton ◽  
P. Stempor ◽  
M. Caron ◽  
A. Appert ◽  
Y. Dong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Finger Protein ◽  
Regulation Of Gene Expression ◽  
Functional Interaction ◽  
Protein Targets ◽  
Coordinate Regulation ◽  
C Elegans ◽  
Finger Protein ◽  
Proteomics Approach ◽  
The Relationship

AbstractThe CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Ly4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss depend on chromatin context, so it is important to understand the relationship between CFP1 and other chromatin factors. Using a proteomics approach, we identified an unexpected link betweenC. elegansCFP-1 and a Rpd3/Sin3 histone deacetylase complex. We find that mutants of CFP-1, SIN-3, and the catalytic subunit SET-2/SET1 have similar phenotypes and misregulate common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 domain and recruits SIN-3 and the HDA-1/HDAC subunit to H3K4me3 enriched promoters. Our results reveal a novel role for CFP-1 in mediating interaction between SET1/COMPASS and a Sin3 HDAC complex at promoters and uncover coordinate regulation of gene expression by chromatin complexes having distinct activities.

scholarly journals A novel POU domain protein which binds to the T-cell receptor beta enhancer.

1993 ◽  
Vol 13 (9) ◽  
pp. 5450-5460 ◽  
Author(s):  
H Messier ◽  
H Brickner ◽  
J Gaikwad ◽  
A Fotedar
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Function ◽  
Cell Receptor ◽  
Pou Domain ◽  
Specific Manner ◽  
T Cell Receptor Beta ◽  
Domain Protein ◽  
Beta Enhancer

POU domain proteins have been implicated in the regulation of a number of lineage-specific genes. Among the first POU domain proteins described were the immunoglobulin octamer-binding proteins Oct-1 and Oct-2. It was therefore of special interest when we identified a novel lymphoid POU domain protein in Southwestern (DNA-protein) screens of T-cell lambda gt11 libraries. This novel POU protein, TCF beta 1, binds in a sequence-specific manner to a critical motif in the T-cell receptor (TCR) beta enhancer. Sequence analysis revealed that TCF beta 1 represents a new class of POU domain proteins which are distantly related to other POU proteins. TCF beta 1 is encoded by multiple exons whose organization is distinct from that of other POU domain proteins. The expression of TCF beta 1 in a tissue-restricted manner and its ability to bind to multiple motifs in the TCR beta enhancer support a role in regulating TCR beta gene expression. The expression of TCF beta 1 in both B and T cells and the ability of recombinant TCF beta 1 to bind octamer and octamer-related motifs suggest that TCF beta 1 has additional roles in lymphoid cell function. The ability of TCF beta 1 to transactivate in a sequence-specific manner is consistent with a role for regulating lymphoid gene expression.

scholarly journals Global Regulation of Hox Gene Expression in C. elegans by a SAM Domain Protein

2003 ◽  
Vol 4 (6) ◽  
pp. 903-915 ◽  
Author(s):  
Hong Zhang ◽  
Ricardo B.R Azevedo ◽  
Robyn Lints ◽  
Christina Doyle ◽  
Yingqi Teng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Global Regulation ◽  
Hox Gene ◽  
C Elegans ◽  
Sam Domain ◽  
Domain Protein

scholarly journals Multidimensional regulation of gene expression in the C. elegans embryo

2012 ◽  
Vol 22 (7) ◽  
pp. 1282-1294 ◽  
Author(s):  
J. I. Murray ◽  
T. J. Boyle ◽  
E. Preston ◽  
D. Vafeados ◽  
B. Mericle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulation Of Gene Expression ◽  
C Elegans

scholarly journals Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Zoë A Hilbert ◽  
Dennis H Kim
Keyword(s):  
Gene Expression ◽  
Decision Making ◽  
Sensory Neurons ◽  
Regulation Of Gene Expression ◽  
Sensory Information ◽  
Specific Expression ◽  
Control Of Gene Expression ◽  
Neuron Pair ◽  
C Elegans ◽  
Male Specific

Animal behavior is directed by the integration of sensory information from internal states and the environment. Neuroendocrine regulation of diverse behaviors of Caenorhabditis elegans is under the control of the DAF-7/TGF-β ligand that is secreted from sensory neurons. Here, we show that C. elegans males exhibit an altered, male-specific expression pattern of daf-7 in the ASJ sensory neuron pair with the onset of reproductive maturity, which functions to promote male-specific mate-searching behavior. Molecular genetic analysis of the switch-like regulation of daf-7 expression in the ASJ neuron pair reveals a hierarchy of regulation among multiple inputs—sex, age, nutritional status, and microbial environment—which function in the modulation of behavior. Our results suggest that regulation of gene expression in sensory neurons can function in the integration of a wide array of sensory information and facilitate decision-making behaviors in C. elegans.

A novel POU domain protein which binds to the T-cell receptor beta enhancer

1993 ◽  
Vol 13 (9) ◽  
pp. 5450-5460
Author(s):  
H Messier ◽  
H Brickner ◽  
J Gaikwad ◽  
A Fotedar
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Function ◽  
Cell Receptor ◽  
Pou Domain ◽  
Specific Manner ◽  
T Cell Receptor Beta ◽  
Domain Protein ◽  
Beta Enhancer

POU domain proteins have been implicated in the regulation of a number of lineage-specific genes. Among the first POU domain proteins described were the immunoglobulin octamer-binding proteins Oct-1 and Oct-2. It was therefore of special interest when we identified a novel lymphoid POU domain protein in Southwestern (DNA-protein) screens of T-cell lambda gt11 libraries. This novel POU protein, TCF beta 1, binds in a sequence-specific manner to a critical motif in the T-cell receptor (TCR) beta enhancer. Sequence analysis revealed that TCF beta 1 represents a new class of POU domain proteins which are distantly related to other POU proteins. TCF beta 1 is encoded by multiple exons whose organization is distinct from that of other POU domain proteins. The expression of TCF beta 1 in a tissue-restricted manner and its ability to bind to multiple motifs in the TCR beta enhancer support a role in regulating TCR beta gene expression. The expression of TCF beta 1 in both B and T cells and the ability of recombinant TCF beta 1 to bind octamer and octamer-related motifs suggest that TCF beta 1 has additional roles in lymphoid cell function. The ability of TCF beta 1 to transactivate in a sequence-specific manner is consistent with a role for regulating lymphoid gene expression.

scholarly journals Regulation of gene expression by repression condensates during development

2020 ◽  
Author(s):  
Nicholas Treen ◽  
Shunsuke F. Shimobayashi ◽  
Jorine Eeftens ◽  
Clifford P. Brangwynne ◽  
Michael S. Levine
Keyword(s):  
Gene Expression ◽  
Protein Interactions ◽  
Transcriptional Repression ◽  
Regulation Of Gene Expression ◽  
Liquid Droplets ◽  
Control Of Gene Expression ◽  
C Elegans ◽  
Exclusion Mechanism ◽  
Transcriptional Corepressors ◽  
Wd40 Repeats

AbstractThere is emerging evidence for transcription condensates in the activation of gene expression1–3. However, there is considerably less information regarding transcriptional repression, despite its pervasive importance in regulating gene expression in development and disease. Here, we explore the role of liquid-liquid phase separation (LLPS) in the organization of the Groucho/TLE (Gro) family of transcriptional corepressors, which interact with a variety of sequence-specific repressors such as Hes/Hairy4. Gro-dependent repressors have been implicated in a variety of developmental processes, including segmentation of the Drosophila embryo and somitogenesis in vertebrates. These repressors bind to specific recognition sequences, but instead of interacting with coactivators (e.g., Mediator) they recruit Gro corepressors5. Gro contains a series of WD40 repeats that are thought to mediate oligomerization6. How putative Hes/Gro oligomers repress transcription has been the subject of numerous studies5, 6. Here we show that Hes/Gro complexes form discrete puncta within nuclei of living Ciona embryos. These puncta rapidly dissolve during the onset of mitosis and reappear in the ensuing cell cycle. Modified Hes/Gro complexes that are unable to bind DNA exhibit the properties of viscous liquid droplets, similar to those underlying the biogenesis of P-granules in C. elegans7 and nucleoli in Xenopus oocytes8. These observations provide vivid evidence for LLPS in the control of gene expression and suggest a simple physical exclusion mechanism for transcriptional repression. WD40 repeats have been implicated in a wide variety of cellular processes in addition to transcriptional repression9. We suggest that protein interactions using WD40 motifs might be a common feature of processes reliant on LLPS.

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