Healing potential of injectable Aloe vera hydrogel loaded by adipose-derived stem cell in skin tissue-engineering in a rat burn wound model

2019 ◽  
Vol 377 (2) ◽  
pp. 215-227 ◽  
Author(s):  
Ahmad Oryan ◽  
Esmat Alemzadeh ◽  
Ali Akbar Mohammadi ◽  
Ali Moshiri
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Aloe Vera ◽  
Skin Tissue ◽  
Burn Wound ◽  
Skin Tissue Engineering ◽  
Wound Model ◽  
Adipose Derived Stem Cell

scholarly journals Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Azar Nourian Dehkordi ◽  
Fatemeh Mirahmadi Babaheydari ◽  
Mohammad Chehelgerdi ◽  
Shiva Raeisi Dehkordi
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Stem Cell ◽  
Therapeutic Strategies ◽  
Skin Tissue ◽  
Skin Tissue Engineering

scholarly journals Skin Tissue Engineering: Application of Adipose-Derived Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Agnes S. Klar ◽  
Jakub Zimoch ◽  
Thomas Biedermann
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Engineering Application ◽  
Stromal Vascular Fraction ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Adipose Derived Stem Cells ◽  
Skin Tissue Engineering ◽  
Stem Cell Source

Perception of the adipose tissue has changed dramatically over the last few decades. Identification of adipose-derived stem cells (ASCs) ultimately transformed paradigm of this tissue from a passive energy depot into a promising stem cell source with properties of self-renewal and multipotential differentiation. As compared to bone marrow-derived stem cells (BMSCs), ASCs are more easily accessible and their isolation yields higher amount of stem cells. Therefore, the ASCs are of high interest for stem cell-based therapies and skin tissue engineering. Currently, freshly isolated stromal vascular fraction (SVF), which may be used directly without any expansion, was also assessed to be highly effective in treating skin radiation injuries, burns, or nonhealing wounds such as diabetic ulcers. In this paper, we review the characteristics of SVF and ASCs and the efficacy of their treatment for skin injuries and disorders.

scholarly journals Synergistic Effect of Biomaterial and Stem Cell for Skin Tissue Engineering in Cutaneous Wound Healing: A Concise Review

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1546
Author(s):  
Shaima Maliha Riha ◽  
Manira Maarof ◽  
Mh Busra Fauzi
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Cell Therapy ◽  
Synergistic Effect ◽  
Stem Cell Therapy ◽  
Skin Tissue ◽  
Therapeutic Effects ◽  
Skin Tissue Engineering

Skin tissue engineering has made remarkable progress in wound healing treatment with the advent of newer fabrication strategies using natural/synthetic polymers and stem cells. Stem cell therapy is used to treat a wide range of injuries and degenerative diseases of the skin. Nevertheless, many related studies demonstrated modest improvement in organ functions due to the low survival rate of transplanted cells at the targeted injured area. Thus, incorporating stem cells into biomaterial offer niches to transplanted stem cells, enhancing their delivery and therapeutic effects. Currently, through the skin tissue engineering approach, many attempts have employed biomaterials as a platform to improve the engraftment of implanted cells and facilitate the function of exogenous cells by mimicking the tissue microenvironment. This review aims to identify the limitations of stem cell therapy in wound healing treatment and potentially highlight how the use of various biomaterials can enhance the therapeutic efficiency of stem cells in tissue regeneration post-implantation. Moreover, the review discusses the combined effects of stem cells and biomaterials in in vitro and in vivo settings followed by identifying the key factors contributing to the treatment outcomes. Apart from stem cells and biomaterials, the role of growth factors and other cellular substitutes used in effective wound healing treatment has been mentioned. In conclusion, the synergistic effect of biomaterials and stem cells provided significant effectiveness in therapeutic outcomes mainly in wound healing improvement.

A New Horizon in Facial Plastic Surgery: Skin Tissue-Engineering and Stem Cell Therapy—Reality or Dream?

2014 ◽  
Vol 31 (3) ◽  
pp. 207-224
Author(s):  
Seied Omid Keyhan ◽  
Seifollah Hemmat ◽  
Mohammad Ali Asayesh ◽  
Peyman Mehriar ◽  
Arash Khojasteh
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Therapy ◽  
Plastic Surgery ◽  
Stem Cell Therapy ◽  
Skin Tissue ◽  
Facial Plastic Surgery ◽  
Skin Tissue Engineering ◽  
Facial Plastic

Aloe vera incorporated biomimetic nanofibrous scaffold: a regenerative approach for skin tissue engineering

2014 ◽  
Vol 23 (3) ◽  
pp. 237-248 ◽  
Author(s):  
S. Suganya ◽  
J. Venugopal ◽  
S. Agnes Mary ◽  
S. Ramakrishna ◽  
B. S. Lakshmi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Aloe Vera ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Regenerative Approach

FABRICATION AND CHARACTERIZATION OF ELECTROSPUN PLLA/COLLAGEN NANOFIBROUS SCAFFOLD COATED WITH CHITOSAN TO SUSTAIN RELEASE OF ALOE VERA GEL FOR SKIN TISSUE ENGINEERING

2016 ◽  
Vol 28 (05) ◽  
pp. 1650035 ◽  
Author(s):  
Majid Salehi ◽  
Saeed Farzamfar ◽  
Farshid Bastami ◽  
Roksana Tajerian
Keyword(s):  
Tissue Engineering ◽  
Sustained Release ◽  
Aloe Vera ◽  
Water Vapor Permeability ◽  
Skin Tissue ◽  
Vapor Permeability ◽  
Mouse Fibroblasts ◽  
Skin Tissue Engineering ◽  
Aloe Vera Gel ◽  
Sustain Release

Background and aim: Healing of fire-induced wounds has been still a challenge in clinical issues. The aim of this study was to fabricate a nanofibrous poly (L-lactic acid)/collagen (PLLA/COL) scaffold with sustained release of aloe vera (AV) gel using a chitosan (CT)-coated layer for skin tissue engineering applications. Material and methods: Morphology, porosity, tensile strength, hydrophilicity, degradation rate, water vapor permeability and water uptake ratio of the scaffold were characterized. The behaviors of mouse fibroblasts (L929) were evaluated on the scaffold. Results: We observed that although the porosity of the scaffold was decreased, other characteristics were enhanced by coating a CT layer. The scaffold supports attachment, viability and proliferation of mouse fibroblasts. Conclusion: Consequently, the PLLA/COL scaffold coated with CT for sustained release of AV gel can be considered as a desirable scaffold for skin tissue engineering.

scholarly journals Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

2019 ◽  
Vol 20 (20) ◽  
pp. 5174 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Raghavendra Ramalingam ◽  
Chetna Dhand ◽  
Rajamani Lakshminarayanan ◽  
Asif Sadiq ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Aloe Vera ◽  
Dermal Fibroblasts ◽  
Tetracycline Hydrochloride ◽  
Skin Tissue ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds ◽  
Diffusion Assay

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.

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