Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis

2009 ◽  
Vol 33 (4) ◽  
pp. 473-483 ◽  
Author(s):  
Ruisong Yu ◽  
Shijuan Dong ◽  
Yumin Zhu ◽  
Hu Jin ◽  
Mingjie Gao ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Cultivation ◽  
Interferon Α ◽  
Fed Batch ◽  
Fed Batch Cultivation

Modeling and Model Based Feeding Control for Pichia pastoris Fed-Batch Cultivation

2004 ◽  
Vol 37 (1) ◽  
pp. 451-456
Author(s):  
H.T. Ren ◽  
J.H. Deng ◽  
B.K. He ◽  
L.M. Ren ◽  
J.Q. Yuan
Keyword(s):  
Pichia Pastoris ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Model Based ◽  
Feeding Control ◽  
Fed Batch Cultivation

scholarly journals Fed-batch cultivation and operational conditions for the production of a recombinant anti-amoebic vaccine in Pichia pastoris system.

2019 ◽  
Vol 19 (2) ◽  
pp. 691-705
Author(s):  
S. L. Martínez-Hernández ◽  
◽  
M. A. Marin-Muñoz ◽  
J. Ventura-Juarez ◽  
J. Jauregui
Keyword(s):  
Pichia Pastoris ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Operational Conditions ◽  
Fed Batch Cultivation

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

A feedforward–feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase

2005 ◽  
Vol 29 (5) ◽  
pp. 1113-1120 ◽  
Author(s):  
M. Arndt ◽  
S. Kleist ◽  
G. Miksch ◽  
K. Friehs ◽  
E. Flaschel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kalman Filter ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation

scholarly journals Fed-Batch Cultivation of Saccharomyces cerevisiae in a Hyperbaric Bioreactor

2003 ◽  
Vol 19 (2) ◽  
pp. 665-671 ◽  
Author(s):  
I. Belo ◽  
R. Pinheiro ◽  
M. Mota
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Cultivation
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