Canola meal as a novel substrate for β-glucosidase production by Trichoderma viride: application of the crude extract to biomass saccharification

2015 ◽  
Vol 38 (10) ◽  
pp. 1889-1902 ◽  
Author(s):  
J. M. Almeida ◽  
V. A. Lima ◽  
P. C. Giloni-Lima ◽  
A. Knob
Keyword(s):  
Crude Extract ◽  
Trichoderma Viride ◽  
Canola Meal ◽  
Biomass Saccharification

Effective and Low-Cost Saccharification of Pineapple Peel by Trichoderma viride Crude Extract with Enhanced β-Glucosidase Activity

2016 ◽  
Vol 9 (3) ◽  
pp. 701-710 ◽  
Author(s):  
Janaína Marques de Almeida ◽  
Vanderlei Aparecido de Lima ◽  
Patrícia Carla Giloni de Lima ◽  
Adriana Knob
Keyword(s):  
Crude Extract ◽  
Low Cost ◽  
Trichoderma Viride ◽  
Glucosidase Activity ◽  
Pineapple Peel

scholarly journals Ozonation Pretreatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State Fermentation

2017 ◽  
Vol 1 (2) ◽  
pp. 27 ◽  
Author(s):  
Efri Mardawati ◽  
Surya Martha Pratiwi ◽  
Robi Andoyo ◽  
Tita Rialita ◽  
Mochamad Djali ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Solid State Fermentation ◽  
Chemical Industry ◽  
Crude Extract ◽  
Incubation Time ◽  
Trichoderma Viride ◽  
Xylanase Activity ◽  
Alternative Substrate ◽  
Xylanase Production

Xylanases are highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. Ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pretreatment (using autoclave, ozonation, combination of ozonation and autoclave, also without pretreatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pretreatment.  Ozonation pretreatment process can increase the enzyme activity of xylanase.

Investigation of lactose hydrolysis by crude extract of Trichoderma viride ATCC 32098

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Isil Seyis Bilkay
Keyword(s):  
Crude Extract ◽  
Trichoderma Viride ◽  
Maximum Efficiency ◽  
Lactose Hydrolysis ◽  
Milk Whey ◽  
Optimum Ph ◽  
Beta Galactosidase ◽  
Rotary Shaker

AbstractObjective:Aim of this study was to find the optimum pH, temperature and incubation conditions for efficient lactose hydrolysis by beta-galactosidase produced byMaterial and methods:ATCC 32098 was incubated at 30°C for 8 days on a rotary shaker. Lactose hydrolysis was calculated based on the amount of glucose measured. Hydrolysis was carried out in lactose solution, milk and whey.Results:Maximum efficiency was observed at pH 5.0 and at 60°C. Agitation increases lactose hydrolysis slightly. When enzymatic lactose hydrolysis in milk, whey and lactose solutions were studied, it was observed that after 26 h, hydrolyses in lactose solution and whey were almost 100% and hydrolysis in milk was 92%.Conclusion:It can be concluded that the enzyme produced from

Genotoxicity evaluation in vitro by comet assay of compound and crude extract obtained from P. Pubescens Benth.

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
VHS Souza ◽  
A Paula OHohne ◽  
R Grando ◽  
N de Cassia de Almeida Queiroz ◽  
GM Pastore ◽  
...  
Keyword(s):  
Comet Assay ◽  
Crude Extract

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

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