The inhibitory effect of the crude extract of Centella asiatica on α-amylase activity

This study was conducted to investigate the effects of the crude extract from Acanthopanax sessiliflorus fruits and the isolated seco-triterpenoids from the crude extract on blood flow in human umbilical vein endothelial cell (HUVEC) invasion assay and angiotensin converting enzyme (ACE) inhibitory activity assay. On the basis of DMSO, the extent of HUVECs'invasion was remarkably decreased with crude extract concentrations of 400 and 1000 μg/mL. Additionally, the extent of the HUVEC invasion inhibitory effect in 400 and 1000 μg/mL of acanthosessilioside F were 55.8% and 72.4%, respectively. In addition, the maximum extent of the HUVEC invasion inhibitory effect of 22-α-hydroxychiisanoside was 88.9%. The IC50 value of the inhibitory effect on ACE activity in the crude extract was 4 μg/mL. The isolated seco-triterpenoids, 22α-hydroxychiisanogenin, 3,4- seco-lupan-20(30)-en-3,28-dioic acid, (1 R)-1,4-epoxy-11α,22α-hydroxy-3,4- seco-lupan-20(30)-en-3,28-dioicacid, (+)-divaroside, and chiisanosidehad showed very high inhibitory effects on ACE activity, ranging from 1.8 to 2.9 üg/mL, which is much higher than the 150.0 üg/mL effect of aspirin. These results suggest that the crude extract from Acanthopanax sessiliflorus fruits and the isolated seco-triterpenoids from the crude extract enhance the blood flow effect by decreasing ACE activity.

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In vitro growth-inhibitory effect of Brazilian plants extracts against Paenibacillus larvae and toxicity in bees

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520140282 ◽

2015 ◽

Vol 87 (2) ◽

pp. 1041-1047 ◽

Cited By ~ 6

Author(s):

Mariana Piana ◽

Thiele F. de Brum ◽

Aline A. Boligon ◽

Camilla F.S. Alves ◽

Robson B. de Freitas ◽

...

Keyword(s):

Crude Extract ◽

Inhibitory Effect ◽

Paenibacillus Larvae ◽

Dilution Method ◽

Minimal Inhibitory Concentrations ◽

Application Method ◽

Growth Inhibitory ◽

Toxicity Analysis ◽

Spreading Disease

American foulbrood (AFB) is a serious worldwide spreading disease in bees caused by Paenibacillus larvae. Plants extracts are known to decrease or inhibit the growth of these bacteria. The purpose of this study was to evaluate the antimicrobial activity of Calendula. officinalis, Cariniana domestica, and Nasturtium officinale extracts against the P. larvae and to evaluate the toxicity of the extracts in bees. In vitro activity against P. larvae of the extracts was evaluated by micro dilution method and the minimal inhibitory concentrations (MICs) were also determined. The concentrations used in the toxicity test were established based on the MIC values and by the spraying application method. The P. larvae was susceptible to the evaluated crude extract of C. officinalis and N. officinale. To C. domestica, only the ethyl acetate (EtAc) fraction and n-butanol (BuOH) fractions had activity against P. larvae. Toxicity analysis in bees showed no toxicity for N. officinale crude extract and for C. domestica BuOH fraction during 15 days of treatment, however, some deaths of bees occurred during the first three days of treatment with C. officinalis and C. domestica EtAc fraction. The results with these species were firstly described and showed that N. officinale crude extract and C. domestica BuOH fraction both presented not toxic effects in the concentration tested by the spraying application method, and can be a useful alternative for treatment or prevention of AFB.

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Inhibitory effect of high leucine concentration on α-amylase secretion by pancreatic acinar cells: possible key factor of proteasome

Bioscience Reports ◽

10.1042/bsr20181455 ◽

2018 ◽

Vol 38 (6) ◽

Cited By ~ 2

Author(s):

Long Guo ◽

Baolong Liu ◽

Chen Zheng ◽

Hanxun Bai ◽

Hao Ren ◽

...

Keyword(s):

Amylase Activity ◽

Acinar Cells ◽

Inhibitory Effect ◽

Proteasome Activity ◽

Pancreatic Acinar Cells ◽

Amylase Secretion ◽

High Concentration ◽

Amylase Release ◽

Cholecystokinin Receptor ◽

Key Factor

The present study aimed to investigate whether leucine affects the pancreatic exocrine by controlling the antisecretory factor (AF) and cholecystokinin receptor (CCKR) expression as well as the proteasome activity in pancreatic acinar cells of dairy calves. The pancreatic acinar cells were isolated from newborn Holstein bull calves and cultured using the Dulbecco’s modified Eagle’s medium/nutrient mixture F12 Ham’s liquid (DMEM/F12). There were six treatments of leucine dosage including 0 (control), 0.23, 0.45, 1.35, 4.05, and 12.15 mM, respectively. After culture for 3 h, the samples were collected for subsequent analysis. As the leucine concentration increased from 0 to 1.35 mM, the α-amylase activity in media decreased significantly (P<0.05), while further increase in leucine concentration did not show any decrease in α-amylase activity. Addition of leucine inhibited (P<0.05) the expression of AF and CCKR, and decreased the activity of proteasome (P<0.05) by 76%, 63%, 24%, 7%, and 9%, respectively. Correlation analysis results showed α-amylase secretion was negatively correlated with leucine concentration (P<0.01), and positively correlated with proteasome activity (P<0.01) and the expression of CCK1R (P<0.01) and AF (P<0.05). The biggest regression coefficient was showed between α-amylase activity and proteasome (0.7699, P<0.001). After inhibition of proteasome by MG-132, low dosage leucine decreased (P<0.05) the activity of proteasome and α-amylase, as well as the expression of CCK1R. In conclusion, we demonstrated that the high-concentration leucine induced decrease in α-amylase release was mainly by decreasing proteasome activity.

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Mangosteen Pericarp Inhibits Nuclear Factor ?B (NF-?B) Activation and Reduces Expression of ICAM-1 in High Cholesterol Diet Rat

Indonesian Journal of Cardiology ◽

10.30701/ijc.v34i1.302 ◽

2013 ◽

pp. 23-32

Author(s):

H Hendarto ◽

Mohammad Saifur Rohman ◽

Djanggan Sargowo

Keyword(s):

Crude Extract ◽

High Fat Diet ◽

Inhibitory Effect ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

High Fat ◽

Factor B ◽

Underlying Risk Factor ◽

Underlying Risk

Background: Atherosclerosis is widely viewed as an inflammatory disease with hypercholesterolemia being a dominant underlying risk factor. This study aimed to determine the effect of mangosteen pericarp in inhibition of NF-?B activation and ICAM-1 expression in rat fed with high cholesterol.Methods and Results: Various doses of crude extract mangosteen pericarp were administered to the high fat diet wistar rats and the activity of NF-?B measured by immunohistochemistry to assess nuclear NF-?B expression and the ICAM-1 expression. The high fat diet resulted significant increased serum LDL levels. Increased nuclear NF- ?B activation and ICAM-1 expression were also observed in high fat diet rats in concurrence with increased serum LDL. The inhibitory effect on NF- ?B activity and ICAM-1 expression was observed when 400 mg of mangosteen pericarp crude extract was administered and even showed a higher inhibitory effect in 800 mg of mangosteen pericap treated rats. The 800 mg extract treatment resulted in decreased ICAM-1 expression similar to those of non high fat rats.Conclusion: The administration of 800 mg mangosteen pericarp crude extract significantly inhibited NF-?B activation and reversed the expression of ICAM -1 to the normal level in high cholesterol diet rats.

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Inhibitory effect of a weight-loss Chinese herbal formula RCM-107 on pancreatic α-amylase activity: Enzymatic and in silico approaches

PLoS ONE ◽

10.1371/journal.pone.0231815 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0231815 ◽

Cited By ~ 1

Author(s):

Shiqi Luo ◽

George Binh Lenon ◽

Harsharn Gill ◽

Andrew Hung ◽

Daniel A. Dias ◽

...

Keyword(s):

Weight Loss ◽

In Silico ◽

Amylase Activity ◽

Inhibitory Effect ◽

Herbal Formula ◽

Chinese Herbal Formula ◽

Chinese Herbal

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Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

Nutrients ◽

10.3390/nu12020411 ◽

2020 ◽

Vol 12 (2) ◽

pp. 411 ◽

Cited By ~ 4

Author(s):

Yonghyeon Lee ◽

Hyeon Kyeong Choi ◽

Kaudjhis Patrick Ulrich N’deh ◽

Young-Jin Choi ◽

Meiqi Fan ◽

...

Keyword(s):

Atopic Dermatitis ◽

Allergic Inflammation ◽

Centella Asiatica ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Interferon Γ ◽

Dependent Manner ◽

Hacat Cells ◽

Signaling Mechanism ◽

Tnf Α

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

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Apparent Inhibitory Effect of Fermented Brown Rice on Salivary Amylase Activity Relative to That of Acarbose, Guava Leaf Tea and Nucleic Acid

Nippon Eiyo Shokuryo Gakkaishi ◽

10.4327/jsnfs.66.141 ◽

2013 ◽

Vol 66 (3) ◽

pp. 141-145

Author(s):

Arinobu Yamauchi

Keyword(s):

Nucleic Acid ◽

Amylase Activity ◽

Brown Rice ◽

Inhibitory Effect ◽

Salivary Amylase ◽

Guava Leaf

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Morphometric Measurements to Quantify the Cerulein Induced Hyperstimulatory Pancreatitis of Rats under the Protective Effect of Lectins

Analytical Cellular Pathology ◽

10.1155/1998/618913 ◽

1998 ◽

Vol 17 (4) ◽

pp. 219-230 ◽

Cited By ~ 6

Author(s):

Ludwig Jonas ◽

Ulrike Mikkat ◽

Anke Witte ◽

Uta Beckmann ◽

Katrin Dölker ◽

...

Keyword(s):

Protective Effect ◽

Amylase Activity ◽

Acinar Cells ◽

Inhibitory Effect ◽

Pancreatic Acinar Cells ◽

Amylase Secretion ◽

Ulex Europaeus ◽

Serum Amylase Activity

In preceding papers we demonstrated an inhibitory effect of wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA) on the cholecystokinin (CCK) binding to the CCK receptor of rat pancreatic cells and also on the CCK induced Ca2+release and α-amylase secretionin vitroas well as on pancreatic secretion of intact ratsin vivo. In the present study we show the same inhibitory effect of both lectins on the cerulein pancreatitis of rats. This acute pancreatitis was induced by supramaximal injections (5 µg/kg/h iv or 10 µg/kg/h ip) of the CCK analogue cerulein in rats every hour. To monitor the degree of pancreatitis, we measured the number and diameter of injury vacuoles in the pancreatic acinar cells as one of the most important signs of this type of pancreatitis by light microscopic morphometry with two different systems on paraffin sections. Furthermore, the serum α-amylase activity was measured biochemically. We found a correlation between the diameter of vacuoles inside the acinar cells and the serum enzyme activity up to 24 h. The simultaneous ip administration of cerulein and WGA or UEA in a dosage of 125 µg/kg/h for 8 h led to a reduction of vacuolar diameter from 13.1 ± 2.0 µm (cerulein) to 7.5 ± 1.1 µm (cerulein + WGA) or 7.2 ± 1.3 µm (cerulein + UEA). The serum amylase activity was reduced from 63.7 ± 15.8 mmol/l \times min (cerulein) to 37.7 ± 11.8 (cerulein + WGA) or 39.4; +52.9; -31.1 (cerulein + UEA-I). Both parameters allow the grading this special type of pancreatitis to demonstrate the protective effect of the lectins.

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