Serum calcium determination with atomic absorption spectrophotometry and colorimetric method: association and predictive equations between them in cattle and sheep samples

Author(s):  
P. D. Katsoulos ◽  
L. V. Athanasiou ◽  
A. Dedousi ◽  
Z. S. Polizopoulou
1971 ◽  
Vol 17 (10) ◽  
pp. 1047-1049 ◽  
Author(s):  
S Raymond Gambino ◽  
Ivelice Fonseca

Abstract Comparable results are obtained when serum calcium is measured by a colorimetric cresolpthalein method on the Technicon SMA 12/60 and by atomic absorption spectrophotometry (Instrumentation Laboratory Model 153 instrument), with strontium as an internal standard. Sera from 250 patients with abnormal calcium concentrations, ranging from 4.9 mg/100 ml to 9.0 mg/100 ml, were measured by each method; the difference between the means for each method was only 0.04 mg/100 ml. The standard deviation of the difference between paired results was ±0.16 mg/100 ml. We were unable to discover any effect of drug or disease on the colorimetric assay, which therefore is suitable for use in large-scale surveys. On the other hand, the more precise atomic absorption spectrophotometry is suitable for use as a reference method. In addition, with an internal standard it is fast and simple, making it suitable for emergency assays of calcium 24 h a day.


1964 ◽  
Vol 10 (10) ◽  
pp. 869-890 ◽  
Author(s):  
Alfred Zettner ◽  
David Seligson

Abstract Atomic absorption spectrophotometry (AAS) was applied to the determination of serum calcium. A special diluent was developed to abolish the effect of absorption depressors. This made possible measurement of calcium in directly diluted serum samples without prior removal of any serum constituents. Values obtained by AAS on calcium oxalate precipitates from serum were identical with those found by the direct dilution technique.


1967 ◽  
Vol 13 (5) ◽  
pp. 388-396 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Stanley Morgenstern

Abstract A procedure is presented for the automated determination of serum calcium by atomic absorption spectrophotometry. The serum sample, diluted with acidified lanthanum dichloride, is dialyzed against 0.1 N hydrochloric acid and a portion of the recipient solution is pumped into the atomizer-burner of the spectrophotometer. Data are presented on the precision and reproducibility of the flow system. Addition of calcium to serum shows a mean recovery of 99%. A comparison of the results of analyses on random specimens by permanganate titration and the automated procedure shows good agreement.


1994 ◽  
Vol 40 (5) ◽  
pp. 781-784 ◽  
Author(s):  
Y Kayamori ◽  
Y Katayama

Abstract We developed a kinetic assay for calcium in serum and urine, based on the activation of porcine pancreatic alpha-amylase (EC 3.2.1.1) with 2-chloro-4-nitrophenyl-alpha-maltotrioside as substrate. The kinetic generation of 2-chloro-4-nitrophenol, monitored at 405 nm, was proportional to the concentration of calcium in serum and urine. The assay was developed and evaluated with the Cobas Bio centrifugal analyzer. The average within-run and between-day imprecision (CV) was 0.96/1.26% and 1.07/1.63%, respectively, for serum calcium and 1.50/2.54% and 1.70/2.64%, respectively, for urine calcium. Results of the proposed method (y) correlated well with those determined by atomic absorption spectrophotometry (x): y(serum) = 1.005x + 0.028 mmol/L (Sy/x = 0.058, r = 0.974, n = 50), and y(urine) = 1.017x-0.115 mmol/L Sy/x = 0.30, r = 0.981, n = 25). We also present data showing that the method is highly sensitive, rapid, relatively free of interference, and amenable to automation.


1990 ◽  
Vol 36 (2) ◽  
pp. 351-354 ◽  
Author(s):  
M W Welch ◽  
D W Hamar ◽  
M J Fettman

Abstract We investigated the suppressive effects of phosphate on calcium determinations with lanthanum-air/acetylene and potassium-nitrous oxide/acetylene methods, and we evaluated the ability of these methods to meet the suggested analytical goals for urine samples. The 20 g/L La-air/acetylene method was the most nearly accurate for predicting the actual calcium concentrations (t-test value = -0.042), followed by the 2 g/L K-nitrous oxide/acetylene method (t-test value = 0.450), 10 g/L La-air/acetylene (t-test value = -0.733), and finally 5 g/L La-air/acetylene (t-test value = -2.446). The dilution used significantly influences the apparent calcium concentration measured with the La-air/acetylene methods.


1967 ◽  
Vol 13 (9) ◽  
pp. 788-796 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Morris Oklander

Abstract The flow system developed for the determination of serum calcium by automated atomic absorption spectrophotometry (AAS) was adapted to the determination of serum magnesium. A comparison of the results of analyses by the present procedure and by an automated fluorometric procedure on identical serum specimens shows excellent agreement.


1970 ◽  
Vol 16 (10) ◽  
pp. 816-819 ◽  
Author(s):  
Christopher S Frings ◽  
Patricia S Cohen ◽  
Lowell B Foster

Abstract An automated method is described for determining serum calcium by measuring the red chromogenic complex formed by calcium and alizarin. About 40 µl of undialyzed serum is added directly to one reagent to pro-duce the color, which follows Beer's law at 599 nm to a concentration of 15.0 mg of calcium per 100 ml, with a coefficient of variation of 2.5%. Serum calcium concentrations, as measured by our method, compare favorably with those by the corresponding manual method and by atomic absorption spectrophotometry


1967 ◽  
Vol 13 (12) ◽  
pp. 1079-1087 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman

Abstract A procedure is described for the automated simultaneous determination of serum calcium and phosphate. Calcium is assayed by atomic absorption spectrophotometry. Phosphate is determined by the molybdenum blue reaction in an improved flow system with a more sensitive reducing agent.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Rizki Andalia ◽  
Mulia Aria Suzanni ◽  
Rini Rini

Lipstick or lip dye is a cosmetic dose that serves to coloring, decorative the lips, as a moisturizing material and protect the lips from exposure the sun to provide optimum results. Lipstick should not contain chemicals such as lead (Pb) because the Pb is a heavy metal that is very dangerous when continuously used on the skin, because it will be absorbed into the blood and attack the body organ causing the onset of disease. According to BPOM that the lead rate on the lipstick does not exceed the permissible limit of 20 mg/kg or 20 ppm.This research aims to know the levels contained in the samples are 4 brands of matte lipstick that are sold in the Aceh market in Banda Aceh City with the method of atomic absorption spectrophotometry (AAS). The results showed that on the 4 brands of lipstick matte contain heavy metal lead (Pb) with a rate still qualified allowed by BPOM  is samples A, B, C, and D, respectively at 0.24 ppm; 0.10 ppm; 2.87 ppm and 1.32 ppm, so that the 4 brands of lipstick matte are still used.


2015 ◽  
Vol 1 (1) ◽  
pp. 41
Author(s):  
Bella Santa Rossi ◽  
Paryanti Paryanti ◽  
Yuli Ristianingsih ◽  
Abubakar Tuhuloula

Pewarnaan pada proses produksi kain sasirangan menghasilkan limbah yang mengandung logam berat, diantaranya adalah timbal (Pb2+) dan kadmium (Cd2+). Penelitian ini bertujuan untuk mengetahui pengaruh proses fitoremediasi terhadap konsentrasi logam Pb2+ dan Cd2+ dari limbah cair industri sasirangan di Kalimantan Selatan, serta mengetahui pertambahan berat basah eceng gondok. Eceng gondok yang sudah dibersihkan dan diaklimatisasi ditanam dalam reaktor berisi larutan limbah dengan konsentrasi 3% (v/v); 9% (v/v); dan 15% (v/v). Sampel diambil setiap 2 hari sekali sebanyak 100 ml dan dianalisis menggunakan AAS (Atomic Absorption Spectrophotometry). Hasil penelitian ini menunjukkan bahwa konsentrasi logam Pb2+ dan Cd2+ dalam limbah setelah proses fitoremediasi terjadi penurunan. Kadar Pb2+ pada konsentrasi 3% (v/v) semula 0,197 ppm menjadi 0,062 ppm, pada konsentrasi 9% (v/v) semula 0,200 ppm menjadi 0,077 ppm dan untuk 15% (v/v) adalah 0,225 ppm menjadi 0,093 ppm. Sedangkan untuk Cd2+ konsentrasi 3% (v/v) semula 0,110 ppm menjadi 0,059 ppm, konsentrasi 9% (v/v) adalah 0,127 ppm menjadi 0,045 ppm dan untuk konsentrasi 15% (v/v) semula 0,144 ppm menjadi 0,047 ppm.


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