Anti-inflammatory, anti-nociceptive and anti-oxidant activities of carvacrol containing leaf extracts of edible Indian borage plant Plectranthus amboinicus: an in vivo and in vitro approach

The methanol, ethanol and chlorofom leaf extracts of Satkara, Citrus assamensis (family: Rutaceae), were subjected to in vitro anti-bacterial, thrombolytic, membrane stabilizing and in vivo anti-inflammatory and antitumor activity tests. The chloroform extract of C. assamensis showed the most important spectrum of activity against Bacillus subtilis, Bacillus cereus, Sarcina lutea among 6 gram positive and against 11 gram negative bacteria at the concentration of 1000 μg/disc, while the range of zones of inhibition were within 7-16 mm. Among the tested three extracts CHCl3 extract showed potent thrombolytic activity and hypotonic solution induced haemolytic activity where the percentages of inhibition were found to be 35% and 55% respectively. All the extracts established significant (p<0.05) anti-inflammatory effect by regulating biphasic inflammatory process induced by carrageenan. The leaf extract dose-dependently and significantly decreases the number of EAC cell count and inhibition of cell growth in comparison to the EAC control and standard. The results obtained in the present study indicate that, C. assamensis leaf can be a potential source of anti-bacterial, thrombolytic, membrane stabilizing, anti-inflammatory and antitumor agents.

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The Anti-Inflammatory and Antioxidant Effects of Sodium Propionate

International Journal of Molecular Sciences ◽

10.3390/ijms21083026 ◽

2020 ◽

Vol 21 (8) ◽

pp. 3026 ◽

Cited By ~ 1

Author(s):

Alessia Filippone ◽

Marika Lanza ◽

Michela Campolo ◽

Giovanna Casili ◽

Irene Paterniti ◽

...

Keyword(s):

Heme Oxygenase 1 ◽

Dependent Manner ◽

Sodium Propionate ◽

Intraplantar Injection ◽

Concentration Dependent Manner ◽

In Vivo Models ◽

Anti Oxidant ◽

Anti Inflammatory

The major end-products of dietary fiber fermentation by gut microbiota are the short-chain fatty acids (SCFAs) acetate, propionate, and butyrate, which have been shown to modulate host metabolism via effects on metabolic pathways at different tissue sites. Several studies showed the inhibitory effects of sodium propionate (SP) on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. We carried out an in vitro model of inflammation on the J774-A1 cell line, by stimulation with lipopolysaccharide (LPS) and H2O2, followed by the pre-treatment with SP at 0.1, 1 mM and 10 mM. To evaluate the effect on acute inflammation and superoxide anion-induced pain, we performed a model of carrageenan (CAR)-induced rat paw inflammation and intraplantar injection of KO2 where rats received SP orally (10, 30, and 100 mg/kg). SP decreased in concentration-dependent-manner the expression of cicloxigenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) following LPS stimulation. SP was able to enhance anti-oxidant enzyme production such as manganese superoxide dismutase (MnSOD) and heme oxygenase-1 (HO-1) following H2O2 stimulation. In in vivo models, SP (30 and 100 mg/kg) reduced paw inflammation and tissue damage after CAR and KO2 injection. Our results demonstrated the anti-inflammatory and anti-oxidant properties of SP; therefore, we propose that SP may be an effective strategy for the treatment of inflammatory diseases.

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In vitro anti-oxidant and in vivo anti-inflammatory activity determination of the methanolic leaves extract of Millettiapachycarpa

Biomedical Research and Therapy ◽

10.7603/s40730-015-0023-z ◽

2015 ◽

Vol 2 (10) ◽

Cited By ~ 1

Author(s):

Shofiul Azam ◽

Prawej Ansari ◽

Mohammad Mamun Ur Rashid ◽

Mohammad Nazmul Alam ◽

Ismail Hussein Ahmed ◽

...

Keyword(s):

Inflammatory Activity ◽

Activity Determination ◽

Anti Oxidant ◽

Anti Inflammatory

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Evaluation of Anti-inflammatory, Anti-pyretic, Analgesic, and Hepatoprotective Properties of Terminalia macroptera

Planta Medica International Open ◽

10.1055/a-1142-7072 ◽

2020 ◽

Vol 07 (02) ◽

pp. e58-e67

Author(s):

Mahamane Haïdara ◽

Adama Dénou ◽

Mohamed Haddad ◽

Aïssata Camara ◽

Korotoumou Traoré ◽

...

Keyword(s):

Traditional Medicine ◽

High Resolution Mass Spectrometry ◽

Biological Activities ◽

Traditional Healers ◽

Potential Candidate ◽

Murine Models ◽

Leaf Extracts ◽

Anti Inflammatory

AbstractIn Mali, improved traditional medicines [“Médicaments Traditionnels Améliorés”] are prepared from traditionally used medicinal plants. Recently, the Department of Traditional Medicine has identified Terminalia macroptera Guill. & Perr. (Combretaceae) as a potential candidate for an improved traditional medicine. T. macroptera is a West African medicinal plant used in Mali against various health disorders, with more than 30 different indications mentioned by traditional healers, including hepatitis, gonorrhea, fever, pain relief, and various infectious diseases (Helicobacter pylori-associated diseases). To date, validation of most of the biological activities of has been mainly carried out in vitro, except for antimalarial activities. In this study, the potential anti-inflammatory, antipyretic, analgesic, and hepatoprotective properties of T. macroptera were investigated in different murine models. Administration of T. macroptera ethanolic root and leaf extracts in rats significantly reduced pyrexia, pain, inflammation, and hepatic marker enzymes such as alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in the different murine models used (p<0.05). A phytochemical screening of T. macroptera revealed the presence of tannins, flavonoids, saponins, anthracene derivatives, sterols, triterpenes, and sugars in both leaf and root extracts as the main phytochemical compounds. This was confirmed by qualitative analysis, liquid chromatography coupled with high-resolution mass spectrometry. T. macroptera extracts demonstrated interesting in vivo antipyretic, analgesic, anti-inflammatory, and hepatoprotective activities. Therefore, T. macroptera should be proposed and further evaluated as a potential improved traditional medicine for the treatment of liver-related disorders and for the relief of pain and fever.

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