Eosinophil efferocytosis during emphysematous eosinophilic lymphangitis in the bovine rumen: histopathological evaluation with special focus on cellular kinetics of professional phagocytes

Author(s):  
Susumu Ohfuji
Bioanalysis ◽  
2020 ◽  
Author(s):  
Tong-yuan Yang ◽  
Rajitha Doddareddy

Real-time quantitative polymerase chain reaction (qPCR) has become the standard method for monitoring cellular kinetics of CAR-T therapies with measurement of the CAR transgene copy numbers in peripheral blood mononuclear cells isolated from patients receiving the treatment. Unlike other biophysical and immunological methodologies for bioanalytical characterization of conventional small molecule drugs or protein biologics, there is no relevant regulatory guidance to date on the method development and validation for quantitative qPCR assays employed during clinical development of CAR-T products. This paper will provide an overview and considerations in the development and validation of a qPCR assay from sample extraction to assay parameters and its implementation in regulated bioanalysis for CAR-T or other types of cell therapies.


Blood ◽  
1970 ◽  
Vol 36 (4) ◽  
pp. 458-469 ◽  
Author(s):  
MARKLEY H. BOYER ◽  
ANTONY BASTEN ◽  
PAUL B. BEESON

Abstract Intravenous inoculation of Trichinella larvae into rats is followed by eosinophilia with a peak at 6 days. Reinjection of the parasites 20 days later results in an enhanced eosinophil response. This phenomenon may be analogous to the anamnestic antibody response which follows reinjection of antigenic material. Various agents known to modify conventional expressions of immunity were tested for their abilities to influence both primary and secondary eosinophil responses. Given in low single doses, methotrexate and cyclophosphamide could suppress the eosinophil response only after larval challenge; busulfan, prednisolone, bacterial endotoxin, Corynebacterium anaerobium and pertussis vaccine were effective only before the inoculum of parasites. The time-dependent action of these agents on eosinophilia is similar to their action on other systems of immune expression. Of further interest was the observation that certain of the agents which were suppressant when given before first larval challenge also appeared to affect "priming" for the second response. Eosinophilia appears to share common features with recognized immune phenomena. The model described may be useful in studying the cellular kinetics of immune responses, the action of immunosuppressive agents and other problems of cell differentiation or proliferation.


2009 ◽  
Vol 71 (6) ◽  
pp. 745-752 ◽  
Author(s):  
Wang-Mei QI ◽  
Kenkichi YAMAMOTO ◽  
Yuh YOKOO ◽  
Hidenori MIYATA ◽  
Kankanam Gamage Sanath UDAYANGA ◽  
...  

1992 ◽  
Vol 29 (4) ◽  
pp. 297-304 ◽  
Author(s):  
Eugen Musch ◽  
Mouhamad Malek ◽  
Jasna Peter-Katalinic ◽  
Heinz Egge ◽  
Hermann Rink ◽  
...  

2019 ◽  
Vol 10 (1) ◽  
pp. 65-76
Author(s):  
Miriam Cleide Cavalcante de Amorim ◽  
Paula Tereza De Souza Silva ◽  
Patricia Silva Barbosa ◽  
Nayara Evelyn Montefusco

The production of starch generates, as a by-product, the cassava wastewater (manipueira), which can be treated by anaerobic digestion to provide biogas and minimize its polluting potential. The most commonly utilized biomass in the anaerobic digestion is the anaerobic sludge. The literature presents, as an alternative to sludge, bovine manure and ruminal fluids, being scarce the studies with the cassava wastewater. This research evaluated the influence of temperature on the microbial ability of cattle and goat rumen in anaerobically biodegrading the manipueira in substitution to the anaerobic sludge. The cattle and goat rumen specific methanogenic activities (SMA) were compared with that of the anaerobic sludge. Subsequently, by using the inoculum which had the best SMA results, cassava wastewater biodegradability tests were performed, investigating the kinetics of the organic matter removal and methane production at 32 ° C and 39 ° C. The bovine rumen presented better results in the SMA (0,315 g COD-CH4 g VSS.d-1) and methane production (1,026 mL). The temperature of 32 °C did not influence the activity of bovine ruminal inoculum as the kinetics of the biodegradation of the manipueira did not differ for the evaluated temperatures (0.1799 d-1 at 32°C and 0.1781 d-1 at 39°C). Bovine rumen achieved glucose reduction of 76% and 80% and methane yield of 77% and 79% for the tests at 32°C and 39°C, respectively. It is inferred that this type of inoculum might be used in reactors of anaerobic digestion processes for the treatment of the cassava wastewater at the ambient temperature of the semiarid region.


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