Impact of mechanical stability on the progress of bone ongrowth on the frame surfaces of a titanium-coated PEEK cage and a 3D porous titanium alloy cage: in vivo analysis using CT color mapping

2021 ◽  
Author(s):  
Takahiro Makino ◽  
Shota Takaneka ◽  
Yusuke Sakai ◽  
Hideki Yoshikawa ◽  
Takashi Kaito
Keyword(s):  
Titanium Alloy ◽  
Mechanical Stability ◽  
Porous Titanium ◽  
Peek Cage ◽  
Color Mapping ◽  
In Vivo Analysis

A Comparison of 1‐ and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study

2018 ◽  
Vol 38 (1) ◽  
pp. 191-202 ◽  
Author(s):  
Lifang Feng ◽  
Xiaohan Liu ◽  
Hongjuan Cao ◽  
Limei Qin ◽  
Wentao Hou ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Porous Titanium ◽  
In Vivo Study ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

2021 ◽  
Vol 9 ◽  
Author(s):  
Youbin Li ◽  
Yuzhe Liu ◽  
Haotian Bai ◽  
Ronghang Li ◽  
Jing Shang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Growth Factor ◽  
Composite Scaffold ◽  
Umbilical Vein ◽  
Tumor Resection ◽  
Bone Defects ◽  
Porous Titanium ◽  
Bone Integration

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.

In vivo analysis of bone-tissue interface in medical grade titanium and porous titanium with and without cenosphere as space holder

Materialia ◽  
2020 ◽  
Vol 9 ◽  
pp. 100623 ◽  
Author(s):  
D. Dutta Majumdar ◽  
V. Kumar ◽  
A. Roychowdhury ◽  
D.P. Mondal ◽  
M. Ghosh ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Porous Titanium ◽  
Space Holder ◽  
In Vivo Analysis ◽  
Tissue Interface ◽  
Medical Grade

scholarly journals In vitro and in vivo evaluations of mechanical properties, biocompatibility and osteogenic ability of sintered porous titanium alloy implant

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36512-36520 ◽  
Author(s):  
Ji Li ◽  
Zhongli Li ◽  
Ruiling Li ◽  
Yueyi Shi ◽  
Haoran Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Titanium Alloy ◽  
Bone Ingrowth ◽  
Porous Titanium ◽  
Porous Ti ◽  
Good Biocompatibility

The sintered porous Ti6Al4V with 75% porosity has optimal mechanical properties, good biocompatibility and osteogenic ability for more bone ingrowth.

scholarly journals MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Liu ◽  
Jinghuan Huang ◽  
Feng Chen ◽  
Dong Xie ◽  
Longqing Wang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Extracellular Vesicles ◽  
Porous Titanium ◽  
Fluorescent Labeling ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Alizarin Red ◽  
Alkaline Phosphatase Staining

Abstract Background Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading to implant failure, implant revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, and we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection and transportation of miR-20a (sEV-20a). In this study, we intended to determine whether sEVs overexpressing miR-20a could exert a superior effect on osteoporotic bone defects and the underlying mechanism. Methods For evaluating the effect of sEV-20a on osteogenesis, in vitro and in vivo studies were performed. In vitro, we first showed that miR-20a was upregulated in the osteogenic process and overexpressed sEVs with miR-20a by the transfection method. Then, the proliferation, migration, and osteogenic differentiation abilities of hBM-MSCs treated with sEV-20a were detected by CCK-8 assays, alkaline phosphatase staining and alizarin red staining, qRT-PCR, and western blot. In vivo, we established an osteoporotic bone defect model and evaluated the effect of sEV-20a on bone formation by micro-CT, sequential fluorescent labeling, and histological analysis. To further explore the mechanism, we applied a bioinformatics method to identify the potential target of miR-20a. Results In vitro, sEV-20a was successfully established and proved to promote the migration and osteogenesis of hBM-MSCs. In vivo, sEV-20a promoted osteointegration in an osteoporotic rat model. To further elucidate the related mechanism, we proved that miR-20a could enhance osteogenesis by targeting BAMBI. Conclusions Collectively, the in vitro and in vivo results confirmed that MSC-derived sEV-20a therapy effectively promoted osteoporotic porous titanium alloy osteointegration via pro-osteogenic effects by targeting BAMBI.

Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study

2017 ◽  
Vol 80 ◽  
pp. 7-17 ◽  
Author(s):  
Hongjuan Cao ◽  
Lifang Feng ◽  
Zhenxian Wu ◽  
Wentao Hou ◽  
Shujun Li ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Porous Titanium ◽  
In Vivo Study ◽  
Biological Behavior ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Bony ingrowth potential of 3D-printed porous titanium alloy: a direct comparison of interbody cage materials in an in vivo ovine lumbar fusion model

2018 ◽  
Vol 18 (7) ◽  
pp. 1250-1260 ◽  
Author(s):  
Kirk C. McGilvray ◽  
Jeremiah Easley ◽  
Howard B. Seim ◽  
Daniel Regan ◽  
Sigurd H. Berven ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Lumbar Fusion ◽  
Porous Titanium ◽  
Interbody Cage ◽  
Fusion Model ◽  
3D Printed

Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space

2020 ◽  
Vol 64 (2) ◽  
pp. 251-261
Author(s):  
Jessica E. Fellmeth ◽  
Kim S. McKim
Keyword(s):  
Chromosome Segregation ◽  
New Technologies ◽  
Early Prophase ◽  
Unique Role ◽  
Kinetochore Assembly ◽  
M Phase ◽  
In Vivo Analysis ◽  
Meiosis I

Abstract While many of the proteins involved in the mitotic centromere and kinetochore are conserved in meiosis, they often gain a novel function due to the unique needs of homolog segregation during meiosis I (MI). CENP-C is a critical component of the centromere for kinetochore assembly in mitosis. Recent work, however, has highlighted the unique features of meiotic CENP-C. Centromere establishment and stability require CENP-C loading at the centromere for CENP-A function. Pre-meiotic loading of proteins necessary for homolog recombination as well as cohesion also rely on CENP-C, as do the main scaffolding components of the kinetochore. Much of this work relies on new technologies that enable in vivo analysis of meiosis like never before. Here, we strive to highlight the unique role of this highly conserved centromere protein that loads on to centromeres prior to M-phase onset, but continues to perform critical functions through chromosome segregation. CENP-C is not merely a structural link between the centromere and the kinetochore, but also a functional one joining the processes of early prophase homolog synapsis to late metaphase kinetochore assembly and signaling.

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