scholarly journals In vitro and in vivo evaluations of mechanical properties, biocompatibility and osteogenic ability of sintered porous titanium alloy implant

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36512-36520 ◽  
Author(s):  
Ji Li ◽  
Zhongli Li ◽  
Ruiling Li ◽  
Yueyi Shi ◽  
Haoran Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Titanium Alloy ◽  
Bone Ingrowth ◽  
Porous Titanium ◽  
Porous Ti ◽  
Good Biocompatibility

The sintered porous Ti6Al4V with 75% porosity has optimal mechanical properties, good biocompatibility and osteogenic ability for more bone ingrowth.

A Comparison of 1‐ and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study

2018 ◽  
Vol 38 (1) ◽  
pp. 191-202 ◽  
Author(s):  
Lifang Feng ◽  
Xiaohan Liu ◽  
Hongjuan Cao ◽  
Limei Qin ◽  
Wentao Hou ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Porous Titanium ◽  
In Vivo Study ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

2021 ◽  
Vol 9 ◽  
Author(s):  
Youbin Li ◽  
Yuzhe Liu ◽  
Haotian Bai ◽  
Ronghang Li ◽  
Jing Shang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Growth Factor ◽  
Composite Scaffold ◽  
Umbilical Vein ◽  
Tumor Resection ◽  
Bone Defects ◽  
Porous Titanium ◽  
Bone Integration

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.

scholarly journals Heat treatment effect on the mechanical properties, roughness and bone ingrowth capacity of 3D printing porous titanium alloy

RSC Advances ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 12471-12483 ◽  
Author(s):  
Zuhao Li ◽  
Chang Liu ◽  
Bingfeng Wang ◽  
Chenyu Wang ◽  
Zhonghan Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Heat Treatment ◽  
Titanium Alloy ◽  
3D Printing ◽  
Mechanical Strength ◽  
Clinical Application ◽  
Treatment Effect ◽  
Bone Ingrowth ◽  
Porous Titanium

The weak mechanical strength and biological inertia of Ti–6Al–4V porous titanium alloy limit its clinical application in the field of orthopedics.

Electrophoretic Deposition of Porous Ti Coatings for Bone Implants: In Vitro and In Vivo Evaluation

2015 ◽  
Vol 654 ◽  
pp. 144-148
Author(s):  
Annabel Braem ◽  
Bram Neirinck ◽  
Omer Van der Biest ◽  
Jef Vleugels
Keyword(s):  
Electrophoretic Deposition ◽  
Bone Ingrowth ◽  
Processing Route ◽  
Porous Ti ◽  
High Adhesion ◽  
Metallurgical Processing ◽  
Plasma Sprayed ◽  
Potential Use

A new powder metallurgical processing route for porous Ti coatings on Ti-6Al-4V substrates based on the electrophoretic deposition (EPD) of TiH2 suspensions is presented. After dehydrogenation and sintering in vacuum, coatings with a fully interconnected porosity (up to 51%, interconnective pore channels (IPC) of 2-50 µm) and high adhesion strength (up to 47 MPa) are obtained. Further evaluation of these coatings for potential use in biomedical implants shows that EPD Ti coatings are significantly less prone to bacterial adhesion compared to state-of-the-art vacuum plasma sprayed (VPS) coatings, while still allowing substantial bone ingrowth. Using EPD, the coating process can easily be transferred to complex-shaped implant components.

scholarly journals MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Liu ◽  
Jinghuan Huang ◽  
Feng Chen ◽  
Dong Xie ◽  
Longqing Wang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Extracellular Vesicles ◽  
Porous Titanium ◽  
Fluorescent Labeling ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Alizarin Red ◽  
Alkaline Phosphatase Staining

Abstract Background Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading to implant failure, implant revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, and we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection and transportation of miR-20a (sEV-20a). In this study, we intended to determine whether sEVs overexpressing miR-20a could exert a superior effect on osteoporotic bone defects and the underlying mechanism. Methods For evaluating the effect of sEV-20a on osteogenesis, in vitro and in vivo studies were performed. In vitro, we first showed that miR-20a was upregulated in the osteogenic process and overexpressed sEVs with miR-20a by the transfection method. Then, the proliferation, migration, and osteogenic differentiation abilities of hBM-MSCs treated with sEV-20a were detected by CCK-8 assays, alkaline phosphatase staining and alizarin red staining, qRT-PCR, and western blot. In vivo, we established an osteoporotic bone defect model and evaluated the effect of sEV-20a on bone formation by micro-CT, sequential fluorescent labeling, and histological analysis. To further explore the mechanism, we applied a bioinformatics method to identify the potential target of miR-20a. Results In vitro, sEV-20a was successfully established and proved to promote the migration and osteogenesis of hBM-MSCs. In vivo, sEV-20a promoted osteointegration in an osteoporotic rat model. To further elucidate the related mechanism, we proved that miR-20a could enhance osteogenesis by targeting BAMBI. Conclusions Collectively, the in vitro and in vivo results confirmed that MSC-derived sEV-20a therapy effectively promoted osteoporotic porous titanium alloy osteointegration via pro-osteogenic effects by targeting BAMBI.

Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study

2017 ◽  
Vol 80 ◽  
pp. 7-17 ◽  
Author(s):  
Hongjuan Cao ◽  
Lifang Feng ◽  
Zhenxian Wu ◽  
Wentao Hou ◽  
Shujun Li ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Porous Titanium ◽  
In Vivo Study ◽  
Biological Behavior ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals The combination of multi-functional ingredients-loaded hydrogels and three-dimensional printed porous titanium alloys for infective bone defect treatment

2020 ◽  
Vol 11 ◽  
pp. 204173142096579
Author(s):  
Shichong Qiao ◽  
Dongle Wu ◽  
Zuhao Li ◽  
Yu Zhu ◽  
Fei Zhan ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Ingrowth ◽  
Three Dimensional ◽  
Supramolecular Assembly ◽  
Porous Titanium ◽  
Novel Therapy ◽  
In Vivo Experiments ◽  
Marrow Mesenchymal Stem Cells

Biomaterial with the dual-functions of bone regeneration and antibacterial is a novel therapy for infective bone defects. Three-dimensional (3D)-printed porous titanium (pTi) benefits bone ingrowth, but its microporous structure conducive to bacteria reproduction. Herein, a multifunctional hydrogel was prepared from dynamic supramolecular assembly of sodium tetraborate (Na2B4O7), polyvinyl alcohol (PVA), silver nanoparticles (AgNPs) and tetraethyl orthosilicate (TEOS), and composited with pTi as an implant system. The pTi scaffolds have ideal pore size and porosity matching with bone, while the supramolecular hydrogel endows pTi scaffolds with antibacterial and biological activity. In vitro assessments indicated the 3D composite implant was biocompatible, promoted bone marrow mesenchymal stem cells (BMSCs) proliferation and osteogenic differentiation, and inhibited bacteria, simultaneously. In vivo experiments further demonstrated that the implant showed effective antibacterial ability while promoting bone regeneration. Besides distal femur defect, the innovative scaffolds may also serve as an ideal biomaterial (e.g. dental implants) for other contaminated defects.

Osteoconductivity of Porous Titanium Having Young’s Modulus Similar to Bone and Surface Modification by OCP

2007 ◽  
Vol 330-332 ◽  
pp. 951-954 ◽  
Author(s):  
Yuko Suzuki ◽  
Naoyuki Nomura ◽  
Shuji Hanada ◽  
Shinji Kamakura ◽  
Takahisa Anada ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Young's Modulus ◽  
Applied Pressure ◽  
Porous Titanium ◽  
Mouse Bone Marrow ◽  
Calvarial Bone ◽  
Coating Agent ◽  
Porous Ti

The present study was designed to investigate whether porous titanium (Ti) having Young’s modulus similar to bone has osteoconductive characteristics in rat critical-sized calvarial bone defect. The effect of coating by octacalcium phosphate (OCP) was also examined. OCP is known as a precursor of initial mineral crystals of biological apatite in bones and teeth. Ti powder was prepared by plasma rotating electrode process in an Ar atmosphere. Then, porous Ti disks, 8 mm in diameter with 1 mm thick, were obtained using the particles ranging from 300 to 500 +m, by sintering at 1573 K without applied pressure. The disks had about 35 vol% in porosity and about 10 GPa in Young’s modulus which corresponds to that of human cortical bone. Newly formed bone was observed so as to fill the pore up at 12 weeks, confirming the ability to conduct the ingrowths of the bone tissue. Although in vitro study showed that proliferation of mouse bone marrow stromal ST-2 cells was inhibited on the dishes coated by OCP rather than the control dish, OCP coating on porous Ti seemed to stimulate the bone formation in vivo. Taken together, it seems likely that porous Ti having Young’s modulus similar to bone shows osteoconductive characteristics to conduct bone ingrowths. OCP could be a potential coating agent to assist bone regeneration on porous Ti.

Plasma-Sprayed Wollastonite Coatings for Biomedical Application

2005 ◽  
Vol 486-487 ◽  
pp. 201-204 ◽  
Author(s):  
Wei Chang Xue ◽  
Xuan Yong Liu ◽  
Xue Bin Zheng ◽  
Chuan Xian Ding
Keyword(s):  
Mechanical Properties ◽  
Body Fluid ◽  
Biomedical Application ◽  
Fibrous Tissue ◽  
New Bone Formation ◽  
Living Bone ◽  
Good Biocompatibility ◽  
Plasma Sprayed

A new bioceramic coating based on wollastonite was prepared by plasma spraying. The coatings exhibited good mechanical properties. The bond strength of the coating on substrate was about 40 MPa, which is higher than that of HA coatings used in orthopedics and dentistry. The bioactivity of wollastonite coatings was evaluated in vitro and in vivo. After immersed in simulated body fluid, a bone-like apatite layer was formed on the surface of wollastonite coatings. Osteoblast could survive and proliferate on the surface of coatings. After implanted in dog’s cortical bone, histological observation demonstrated that bone tissue could extend and grow along the surface of wollastonite coatings. The coating bonded directly to bone without any fibrous tissue, indicating good biocompatibility and bone conductivity. The wollastonite coatings also showed good bone inductivity property, inducing new-bone formation on their surface after implanted in marrow. The results obtained indicated that the plasma-sprayed wollastonite coatings possessed good mechanical properties and excellent bioactivity in vitro and in vivo. It appears that a wollastonite coating may be suitable for the repair and replacement of living bone, especially for load-bearing situations.

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