Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin

2015 ◽  
Vol 182 (7-8) ◽  
pp. 1465-1472 ◽  
Author(s):  
Yi-Zhi Wang ◽  
Dong-Yan Li ◽  
Xi-Wen He ◽  
Wen-You Li ◽  
Yu-Kui Zhang
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Polymer Nanoparticles ◽  
Imprinted Polymer ◽  
Specific Recognition

scholarly journals QCM-based assay designs for human serum albumin

2021 ◽  
Author(s):  
Wisnu Arfian A. Sudjarwo ◽  
Mathias Thomas Dobler ◽  
Peter A. Lieberzeit
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Polymer Nanoparticles ◽  
Dependent Manner ◽  
Imprinted Polymer ◽  
Limit Of Quantification ◽  
Concentration Dependent Manner

AbstractSolid-phase synthesis is an elegant way to create molecularly imprinted polymer nanoparticles (nano-MIPs) comprising a single binding site, i.e. mimics of antibodies. When using human serum albumin (HSA) as the template, one achieves nano-MIPs with 53 ± 19 nm diameter, while non-imprinted polymer nanoparticles (nano-NIPs) reach 191 ± 96 nm. Fluorescence assays lead to Stern–Volmer plots revealing selective binding to HSA with selectivity factors of 1.2 compared to bovine serum albumin (BSA), 1.9 for lysozyme, and 4.1 for pepsin. Direct quartz crystal microbalance (QCM) assays confirm these results: nano-MIPs bind to HSA immobilized on QCM surfaces. This opens the way for competitive QCM-based assays for HSA: adding HSA to nanoparticle solutions indeed reduces binding to the QCM surfaces in a concentration-dependent manner. They achieve a limit of detection (LoD) of 80 nM and a limit of quantification (LoQ) of 244 nM. Furthermore, the assay shows recovery rates around 100% for HSA even in the presence of competing analytes.

Human serum albumin-specific recognition of the natural herbal extract of Stryphnodendron polyphyllum through STD NMR, hyphenations and docking simulation studies

RSC Advances ◽  
2015 ◽  
Vol 5 (30) ◽  
pp. 23431-23442 ◽  
Author(s):  
Sheraz A. K. Tanoli ◽  
Nazish U. Tanoli ◽  
Tatiani M. Bondancia ◽  
Saman Usmani ◽  
Zaheer Ul-Haq ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Docking Simulation ◽  
Herbal Extract ◽  
Rapid Screening ◽  
Simulation Studies ◽  
Specific Recognition ◽  
Screening And Identification ◽  
Std Nmr

Over the last two decades, new and more advanced strategies that help in the rapid screening and identification of new ligands for a specific macromolecule have become an important domain.

CdTe quantum dots (QDs) improve the affinities of baicalein and genistein for human serum albumin in vitro

2010 ◽  
Vol 104 (11) ◽  
pp. 1148-1155 ◽  
Author(s):  
Jianbo Xiao ◽  
Tingting Chen ◽  
Longsheng Chen ◽  
Hui Cao ◽  
Fan Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cdte Quantum Dots

Study on the Interaction Between Human Serum Albumin and CdTe Quantum Dots

2013 ◽  
Vol 44 (3) ◽  
pp. 358-363 ◽  
Author(s):  
Xuan Ma ◽  
Lusha Liu ◽  
Xuhua Liang ◽  
Bo Zhao ◽  
Lin Shi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cdte Quantum Dots

Thermodynamics of the Interaction Between Graphene Quantum Dots with Human Serum Albumin and γ-Globulins

2020 ◽  
Vol 49 (1) ◽  
pp. 100-116 ◽  
Author(s):  
Xiao-Xu Ba ◽  
Tian Gao ◽  
Mian Yang ◽  
Peng Jiang ◽  
Feng-Lei Jiang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Graphene Quantum Dots ◽  
Γ Globulins

Self-assembled nanosensor based on squaraine dye for specific recognition and detection of human serum albumin

2018 ◽  
Vol 255 ◽  
pp. 977-985 ◽  
Author(s):  
Yanyan Guo ◽  
Yiping Chen ◽  
Xiaochan Zhu ◽  
Zhizhen Pan ◽  
Xiangyu Zhang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Specific Recognition ◽  
Squaraine Dye ◽  
Self Assembled

Molecular interaction investigation between three CdTe:Zn 2+ quantum dots and human serum albumin: A comparative study

2015 ◽  
Vol 136 ◽  
pp. 955-962 ◽  
Author(s):  
Shan Huang ◽  
Hangna Qiu ◽  
Yi Liu ◽  
Chusheng Huang ◽  
Jiarong Sheng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Comparative Study ◽  
Serum Albumin ◽  
Human Serum ◽  
Molecular Interaction
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