Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature

2019 ◽  
Vol 186 (8) ◽  
Author(s):  
Xiao-ai Zhang ◽  
Wei Zhang ◽  
Qi Wang ◽  
Junli Wang ◽  
Guodong Ren ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Ph Values

Effect of the Association of Eosin Molecules on Their Interaction with Bovine Serum Albumin at Various pH Values

2019 ◽  
Vol 86 (5) ◽  
pp. 855-860
Author(s):  
E. S. Gorodnichev ◽  
A. A. Kuleshova ◽  
A. V. Bykov ◽  
A. M. Saletsky
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Ph Values

Construction of Polysiloxane-Based Fluorescent Probe for Visualizing The pH Down-regulation

2021 ◽  
Author(s):  
Xiaoni Wang ◽  
Zhiming Gou ◽  
Minggang Tian ◽  
Yujing Zuo
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Cell Apoptosis ◽  
Pathological Process ◽  
Down Regulation ◽  
Ph Values

Intracellular pH values change is a significant physiological and pathological process and plays vital roles in autophagy, self-repairing, and programmed cell apoptosis. A unique fluorescent probe (PN-1) based on polysiloxanes...

Bioceramic Hydroxyapatite Granules for Purification of Biotechnological Products

2011 ◽  
Vol 284-286 ◽  
pp. 1764-1769 ◽  
Author(s):  
Vitalijs Lakevics ◽  
Janis Locs ◽  
Dagnija Loca ◽  
Valentina Stepanova ◽  
Liga Berzina-Cimdina ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Phosphate Buffer Solution ◽  
Sorption Process ◽  
Buffer Solution ◽  
Ph Values ◽  
Bovine Serum Albumin Adsorption ◽  
Albumin Adsorption

Sorption experiments of bovine serum albumin (BSA) on hydroxyapatite (HAp) ceramic granules, prepared at three temperatures 900°C, 1000°C and 1150°C were performed at room temperature 18,6 °C and phosphate buffer, pH 5,83; 6.38 and 7,39. Thermal treatment contributed to the decrease of bovine serum albumin immobilization indicating that sorption process depended on HAp ceramics specific surface area and pH values of phosphate buffer solution. However, it was confirmed that granule size was also an important parameter for bovine serum albumin adsorption. As a result of these experiments, the most appropriate adsorption conditions and phosphate buffer pH values influence on to BSA sorption were analyzed.

The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Phosphate/Polyacrylate/Alginate Hybrid Polymer Microspheres

2010 ◽  
Vol 152-153 ◽  
pp. 1636-1640 ◽  
Author(s):  
Kong Yin Zhao ◽  
Jun Fu Wei ◽  
Jin Yang Zhou ◽  
Yi Ping Zhao ◽  
Guo Xiang Cheng
Keyword(s):  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polymer Microspheres ◽  
Hybrid Polymer ◽  
Ph Values ◽  
Factors Influencing ◽  
Recognition Property ◽  
Template Protein

Calcium phosphate/polyacrylate/alginate hybrid polymer microspheres with bovine serum albumin (BSA) embedded and coated on the surface were prepared with (NH4)2HPO4, sodium polyacrylate (SPA) and sodium alginate (SA) via Ca2+ crosslinking in inverse suspension. Rebinding behaviors of the microspheres were evaluated. The factors influencing the imprinting efficiency (IE) of imprinted microspheres were also studied, including the concentration of CaCl2, template content and pH values in rebinding solutions. Selectivity tests showed that the imprinted microspheres exhibited good recognition property for the template protein.

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

Intracellular pH of isolated perfused turtle heart

1960 ◽  
Vol 199 (6) ◽  
pp. 1112-1114 ◽  
Author(s):  
William J. Waddell ◽  
Harold F. Hardman
Keyword(s):  
Intracellular Ph ◽  
Salt Solution ◽  
Weak Acid ◽  
Extracellular Ph ◽  
Chrysemys Picta ◽  
Ph Values ◽  
Painted Turtles ◽  
Turtle Heart

Hearts from 15 painted turtles ( Chrysemys picta) were perfused for 30 minutes at 23°–26°C with a buffered salt solution. The pH of the perfusing solution was varied between 6.5 and 9.5. The intracellular pH values of the ventricles were calculated from the distribution of the weak acid, 5.5-dimethyl-2,4-oxazolidinedione (DMO). It was found that the extracellular pH (pH e) and the intracellular pH (pH i) under these conditions could be related by the equation: pH i = 0.617 pH e + 2.98.

Growth-related changes of phosphorus metabolites in VX-2 carcinoma implanted into rabbit thigh muscle: in vivo 31P MR spectroscopy

2016 ◽  
Author(s):  
Hyeong-Kil Kim ◽  
Gwang Woo Jeong ◽  
Tae-Hoon Kim ◽  
Gwang-Won Kim ◽  
Jong-Bong Kim
Keyword(s):  
Tumor Growth ◽  
Intracellular Ph ◽  
Tumor Necrosis ◽  
Time Course ◽  
Ph Value ◽  
Thigh Muscle ◽  
Ph Values ◽  
Concentration Changes ◽  
Phosphorus Metabolites

The purpose of this study was to evaluate the usefulness of in vivo 31P magnetic resonance spectroscopy (MRS) for monitoring changes in growth-related phosphate metabolite concentration and intracellular pH value in rabbit thigh muscle implanted with VX-2 carcinoma. The time-course magnetic resonance imaging (MRI) and in vivo 31P MRS were examined weekly in the course of 10 weeks following the onset of a VX-2 carcinoma implantation. The spectra were quantitatively analyzed to obtain vital information on the time course variation of the phosphorus metabolites and intracellular pH value according to the tumor growth. Elevation in the concentrations of phosphormonoesters (PME), inorganic phosphate (Pi), and phosphodiesters (PDE) was observed over the time course of 3-4 weeks after the implantation of VX2 carcinoma, while the rest of the metabolites, PCr and ATP tended to be constant. The concentration changes of PME, Pi, and PDE were positively correlated with the volumes of tumor necrosis. The intracellular pH values decreased with the time course of tumor growth and the volumes of tumor necrosis. In vivo 31P MRS is capable of non-invasive monitoring of intracellular pH values as well as the concentration changes of phosphate metabolites during tumor growth.

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