Determination of β-amyloid oligomer using electrochemiluminescent aptasensor with signal enhancement by AuNP/MOF nanocomposite

2021 ◽  
Vol 188 (2) ◽  
Author(s):  
Lixiu Yin ◽  
Yueju Wang ◽  
Rong Tan ◽  
Huiling Li ◽  
Yifeng Tu
Keyword(s):  
Signal Enhancement ◽  
Β Amyloid ◽  
Amyloid Oligomer

Determination of tau proteins and β-amyloid 42 in cerebrospinal fluid by ELISA methods and indicative normative values

2019 ◽  
Vol 82/115 (5) ◽  
pp. 533-540
Author(s):  
Aleš Bartoš ◽  
Magdalena Smětáková ◽  
Libuše Nosková ◽  
Jan Říčný ◽  
Lenka Fialová
Keyword(s):  
Cerebrospinal Fluid ◽  
Tau Proteins ◽  
Normative Values ◽  
Β Amyloid

Fucoxanthin Inhibits β-Amyloid Assembly and Attenuates β-Amyloid Oligomer-Induced Cognitive Impairments

2017 ◽  
Vol 65 (20) ◽  
pp. 4092-4102 ◽  
Author(s):  
Siying Xiang ◽  
Fufeng Liu ◽  
Jiajia Lin ◽  
Huixin Chen ◽  
Chunhui Huang ◽  
...  
Keyword(s):  
Cognitive Impairments ◽  
Β Amyloid ◽  
Amyloid Assembly ◽  
Amyloid Oligomer

Method for the determination of the levels of β-amyloid peptide in the CSF sampled from freely moving rats

2005 ◽  
Vol 52 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Mohammed El Mouedden ◽  
Marc Haseldonckx ◽  
Claire Mackie ◽  
Theo Meert ◽  
Marc Mercken
Keyword(s):  
Amyloid Peptide ◽  
Freely Moving Rats ◽  
Β Amyloid ◽  
Freely Moving ◽  
Β Amyloid Peptide

scholarly journals Determination of Tobramycin in M9 Medium by LC-MS/MS: Signal Enhancement by Trichloroacetic Acid

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Liusheng Huang ◽  
Janus Anders Juul Haagensen ◽  
Davide Verotta ◽  
Vincent Cheah ◽  
Alfred M. Spormann ◽  
...  
Keyword(s):  
Calibration Curve ◽  
Trifluoroacetic Acid ◽  
Trichloroacetic Acid ◽  
Bolus Dose ◽  
Internal Standard ◽  
Signal Enhancement ◽  
Bacterial Biofilms ◽  
Gradient Mode

It is well known that ion-pairing reagents cause ion suppression in LC-MS/MS methods. Here, we report that trichloroacetic acid increases the MS signal of tobramycin. To support studies of an in vitro pharmacokinetic/pharmacodynamic simulator for bacterial biofilms, an LC-MS/MS method for determination of tobramycin in M9 media was developed. Aliquots of 25 μL M9 media samples were mixed with the internal standard (IS) tobramycin-d5 (5 µg/mL, 25 µL) and 200 µL 2.5% trichloroacetic acid. The mixture (5 µL) was directly injected onto a PFP column (2.0 × 50 mm, 3 µm) eluted with water containing 20 mM ammonium formate and 0.14% trifluoroacetic acid and acetonitrile containing 0.1% trifluoroacetic acid in a gradient mode. ESI+ and MRM with ion m/z 468 → 324 for tobramycin and m/z 473 → 327 for the IS were used for quantification. The calibration curve concentration range was 50–25000 ng/mL. Matrix effect from M9 media was not significant when compared with injection solvents, but signal enhancement by trichloroacetic acid was significant (∼3 fold). The method is simple, fast, and reliable. Using the method, the in vitro PK/PD model was tested with one bolus dose of tobramycin.

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