Cowpea golden mosaic disease in Gujarat is caused by a Mungbean yellow mosaic India virus isolate with a DNA B variant

2008 ◽  
Vol 153 (7) ◽  
pp. 1359-1365 ◽  
Author(s):  
P. John ◽  
P. N. Sivalingam ◽  
Q. M. I. Haq ◽  
N. Kumar ◽  
A. Mishra ◽  
...  
Keyword(s):  
Virus Isolate ◽  
Mosaic Disease

Molecular identification of an Ageratum enation virus isolate associated with mosaic disease of pointed gourd (Trichosanthes dioica) in India

2011 ◽  
Vol 39 (5) ◽  
pp. 497-502 ◽  
Author(s):  
S. K. Raj ◽  
S. K. Snehi ◽  
M. S. Khan ◽  
A. K. Tiwari ◽  
G. P. Rao
Keyword(s):  
Molecular Identification ◽  
Virus Isolate ◽  
Mosaic Disease ◽  
Trichosanthes Dioica ◽  
Pointed Gourd

Morphogenesis of a New Human Herpes-Type Virus Isolate (Sasha Virus)

1973 ◽  
Vol 31 ◽  
pp. 592-593
Author(s):  
R. F. Zeigel ◽  
W. Munyon
Keyword(s):  
Virus Isolate ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Human Herpes ◽  
Human Embryonic Lung ◽  
Human Herpes Virus ◽  
Intracytoplasmic Inclusions ◽  
Hel Cells ◽  
And Control

In continuing studies on the role of viruses in biochemical transformation, Dr. Munyon has succeeded in isolating a highly infectious human herpes virus. Fluids of buccal pustular lesions from Sasha Munyon (10 mo. old) uiere introduced into monolayer sheets of human embryonic lung (HEL) cell cultures propagated in Eagles’ medium containing 5% calf serum. After 18 hours the cells exhibited a dramatic C.P.E. (intranuclear vacuoles, peripheral patching of chromatin, intracytoplasmic inclusions). Control HEL cells failed to reflect similar changes. Infected and control HEL cells were scraped from plastic flasks at 18 hrs. of incubation and centrifuged at 1200 × g for 15 min. Resultant cell packs uiere fixed in Dalton's chrome osmium, and post-fixed in aqueous uranyl acetate. Figure 1 illustrates typical hexagonal herpes-type nucleocapsids within the intranuclear virogenic regions. The nucleocapsids are approximately 100 nm in diameter. Nuclear membrane “translocation” (budding) uias observed.

The ultrastructure of the double membrane-bound bodies and endoplasmic reticulum in serial sections of wheat spot mosaic-affected wheat plants

1990 ◽  
Vol 48 (3) ◽  
pp. 694-695
Author(s):  
M. H. Chen ◽  
C. Hiruki
Keyword(s):  
Endoplasmic Reticulum ◽  
High Resolution ◽  
Membrane Structure ◽  
Mosaic Disease ◽  
Serial Sections ◽  
Thin Sections ◽  
Double Membrane ◽  
Southern Alberta ◽  
Membrane Bound ◽  
Scanning Em

Wheat spot mosaic disease was first discovered in southern Alberta, Canada, in 1956. A hitherto unidentified disease-causing agent, transmitted by the eriophyid mite, caused chlorosis, stunting and finally severe necrosis resulting in the death of the affected plants. Double membrane-bound bodies (DMBB), 0.1-0.2 μm in diameter were found to be associated with the disease.Young tissues of leaf and root from 4-wk-old infected wheat plants were fixed, dehydrated, and embedded in Spurr’s resin. Serial sections were collected on slot copper grids and stained. The thin sections were then examined with a Hitachi H-7000 TEM at 75 kV. The membrane structure of the DMBBs was studied by numbering them individually and tracing along the sections to see any physical connection with endoplasmic reticulum (ER) membranes. For high resolution scanning EM, a modification of Tanaka’s method was used. The specimens were examined with a Hitachi Model S-570 SEM in its high resolution mode at 20 kV.

scholarly journals Specificity and Neutralizing Capacity of Three Monoclonal Antibodies Produced against the Envelope Glycoprotein of Simian Immunodeficiency Virus Isolate 251

Virology ◽  
1995 ◽  
Vol 211 (1) ◽  
pp. 339-344 ◽  
Author(s):  
Tahar Babas ◽  
Besma Belhadj-Jrad ◽  
Roger Le Grand ◽  
Dominique Dormont ◽  
Luc Montagnier ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simian Immunodeficiency Virus ◽  
Envelope Glycoprotein ◽  
Virus Isolate ◽  
Neutralizing Capacity ◽  
Immunodeficiency Virus
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