Complete genome analysis of the novel Enterococcus faecalis phage vB_EfaS_AL3

2019 ◽  
Vol 164 (10) ◽  
pp. 2599-2603 ◽  
Author(s):  
Yuyu Yuan ◽  
Feiyang Zhao ◽  
Lili Wang ◽  
Demeng Tan ◽  
Cong Cong ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Genome Analysis ◽  
Complete Genome ◽  
The Novel ◽  
Complete Genome Analysis

Complete genome analysis of the novel Edwardsiella tarda phage vB_EtaM_ET-ABTNL-9

2020 ◽  
Vol 165 (5) ◽  
pp. 1241-1244
Author(s):  
Huijing Cui ◽  
Jiancheng Zhang ◽  
Cong Cong ◽  
Lili Wang ◽  
Xiaoyu Li ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Edwardsiella Tarda ◽  
The Novel ◽  
Complete Genome Analysis

scholarly journals Hybrid genome assembly of Shigella sonnei reveals the novel finding of chromosomal integration of an IncFII plasmid carrying a mphA gene

2020 ◽  
Author(s):  
Dhiviya Prabaa Muthuirulandi Sethuvel ◽  
Shalini Anandan ◽  
Dhivya Murugan ◽  
Kalaiarasi Asokan ◽  
Karthick Vasudevan ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Genome Analysis ◽  
Complete Genome ◽  
Type Species ◽  
Shigella Sonnei ◽  
Diffusion Method ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Complete Genome Analysis

Azithromycin is increasingly being used for the treatment of shigellosis despite a lack of interpretative guidelines and with limited clinical evidence. The present study determined azithromycin susceptibility and correlated this with macrolide-resistance genes in Shigella spp. isolated from stool specimens in Vellore, India. The susceptibility of 332 Shigella isolates to azithromycin was determined using the disc diffusion method. Of these, 31 isolates were found to be azithromycin resistant. The azithromycin minimum inhibitory concentration (MIC) was determined using the broth microdilution method. In addition, isolates were screened for mphA and ermB genes using conventional PCR. Furthermore, an isolate that was positive for resistance genes was subjected to complete genome analysis, and was analysed for mobile genetic elements. The azithromycin MIC for the 31 resistant Shigella isolates ranged between 2 and 16 mg l−1. PCR results showed that a single isolate of Shigella sonnei carried a mphA gene. Complete genome analysis revealed integration of an IncFII plasmid into the chromosome of S. sonnei , which was also found to carry the following resistance genes: sul1, bla DHA1, qnrB4, mphA, tetR. Mutations in the quinolone-resistance-determining region (QRDR) were also observed. Additionally, prophages, insertion sequences and integrons were identified. The novel finding of IncFII plasmid integration into the chromosome of S. sonnei highlights the potential risk of Shigella spp. becoming resistance to azithromycin in the future. These suggests that it is imperative to monitor Shigella susceptibility and to study the resistance mechanism of Shigella to azithromycin considering the limited treatment choices for shigellosis.

scholarly journals Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

2018 ◽  
Vol 12 (1) ◽  
pp. e0006198 ◽  
Author(s):  
Rama Dhenni ◽  
Mulya Rahma Karyanti ◽  
Nina Dwi Putri ◽  
Benediktus Yohan ◽  
Frilasita A. Yudhaputri ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Dengue Virus ◽  
Genome Analysis ◽  
Complete Genome ◽  
Virus Serotype ◽  
Complete Genome Analysis

Identification and complete genome analysis of a virus variant or putative new foveavirus associated with apple green crinkle disease

2013 ◽  
Vol 158 (9) ◽  
pp. 1877-1887 ◽  
Author(s):  
D. James ◽  
A. Varga ◽  
G. D. Jesperson ◽  
M. Navratil ◽  
D. Safarova ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Virus Variant ◽  
Complete Genome Analysis
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