Characteristics and complete genome analysis of a novel jumbo phage infecting pathogenic Bacillus pumilus causing ginger rhizome rot disease

2016 ◽  
Vol 161 (12) ◽  
pp. 3597-3600 ◽  
Author(s):  
Yihui Yuan ◽  
Meiying Gao
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Bacillus Pumilus ◽  
Ginger Rhizome ◽  
Rot Disease ◽  
Complete Genome Analysis ◽  
Rhizome Rot

scholarly journals Effects of Actin-Like Proteins Encoded by Two Bacillus pumilus Phages on Unstable Lysogeny, Revealed by Genomic Analysis

2014 ◽  
Vol 81 (1) ◽  
pp. 339-350 ◽  
Author(s):  
Yihui Yuan ◽  
Qin Peng ◽  
Dandan Wu ◽  
Zheng Kou ◽  
Yan Wu ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Protein A ◽  
Genomic Analysis ◽  
Proteome Analysis ◽  
Control Effect ◽  
Content Type ◽  
Ginger Rhizome ◽  
The Difference ◽  
Rot Disease ◽  
Rhizome Rot

ABSTRACTWe characterized two newly isolated myoviruses, Bp8p-C and Bp8p-T, infecting the ginger rhizome rot disease pathogenBacillus pumilusGR8. The plaque of Bp8p-T exhibited a clear center with a turbid rim, suggesting that Bp8p-T could transform into latent phage. Lysogeny assays showed that both the two phages could form latent states, while Bp8p-T could form latent phage at a higher frequency and stability than Bp8p-C. The genomes of Bp8p-C and Bp8p-T were 151,417 and 151,419 bp, respectively; both encoded 212 putative proteins, and only differed by three nucleotides. Moreover, owing to this difference, Bp8p-C encoded a truncated, putative actin-like plasmid segregation protein Gp27-C. Functional analysis of protein Gp27 showed that Gp27-T encoded by Bp8p-T exhibited higher ATPase activity and assembly ability than Gp27-C. The results indicate that the difference in Gp27 affected the phage lysogenic ability. Structural proteome analysis of Bp8p-C virion resulted in the identification of 14 structural proteins, among which a pectin lyase-like protein, a putative poly-gamma-glutamate hydrolase, and three proteins with unknown function, were firstly identified as components of the phage virion. Both phages exhibited specific lytic ability to the host strain GR8. Bp8p-C showed better control effect on the pathogen in ginger rhizome slices than Bp8p-T, suggesting that Bp8p-C has a potential application in bio-control of ginger rhizome rot disease.

scholarly journals Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

2018 ◽  
Vol 12 (1) ◽  
pp. e0006198 ◽  
Author(s):  
Rama Dhenni ◽  
Mulya Rahma Karyanti ◽  
Nina Dwi Putri ◽  
Benediktus Yohan ◽  
Frilasita A. Yudhaputri ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Dengue Virus ◽  
Genome Analysis ◽  
Complete Genome ◽  
Virus Serotype ◽  
Complete Genome Analysis

Identification and complete genome analysis of a virus variant or putative new foveavirus associated with apple green crinkle disease

2013 ◽  
Vol 158 (9) ◽  
pp. 1877-1887 ◽  
Author(s):  
D. James ◽  
A. Varga ◽  
G. D. Jesperson ◽  
M. Navratil ◽  
D. Safarova ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Virus Variant ◽  
Complete Genome Analysis

scholarly journals Intensitas dan Luas Serangan Beberapa Isolat Fusarium oxysporum f.sp. zingiberi pada Jahe Gajah

2017 ◽  
Vol 21 (1) ◽  
pp. 16
Author(s):  
Hermawati Cahyaningrum ◽  
Nur Prihatiningsih ◽  
Soedarmono Soedarmono
Keyword(s):  
Fusarium Oxysporum ◽  
Sterile Soil ◽  
Soil Medium ◽  
Disease Symptoms ◽  
Ginger Rhizome ◽  
Incubation Periods ◽  
Rot Disease ◽  
Screen House ◽  
Rhizome Rot ◽  
Growth Ability

Ginger is one of the spices and medicinal commodities which is cultivated in Indonesia. One of the obstacles encountered in the cultivation of ginger is the rhizome rot disease which is mainly caused by Fusarium oxysporum Schlecht f.sp. zingiberi Trujillo. This study is aimed to know the growth ability and virulence level of the isolates on ginger rhizome and plants. The research was conducted in the laboratory and in the screen house by using Complete Random Design consisted of 10 treatments and 4 replications. The parameters observed were growth ability of F. oxysporum f.sp. zingiberi, rhizome rot disease symptoms, incubation period, extensive decay and weight difference of the rhizomes. The results showed that F. oxysporum f.sp. zingiberi which was stored for 4 years in sterile soil medium was still capable to cause damage to the rhizome and plants. Incubation periods of rhizome decay and plant symptoms were from 3 to 11.5 and 55.5 to 68.5 days, respectively. The most virulent isolate was MSO1 with extensive decay of rhizome and the wilting intensity were 108.95 mm2 dan 33.88%, respectively. IntisariJahe merupakan salah satu komoditas rempah dan obat yang banyak dibudidayakan di Indonesia. Salah satu kendala yang dihadapi dalam budidaya jahe adalah adanya gangguan penyakit busuk rimpang yang disebabkan (terutama) oleh Fusarium oxysporum Schlecht f.sp. zingiberi Trujillo. Penelitian bertujuan untuk menguji daya tumbuh dan virulensi isolat F. oxysporum f.sp. zingiberi pada rimpang dan tanaman jahe gajah. Penelitian dilakukan di laboratorium dan di rumah kasa menggunakan Rancangan Acak Kelompok Lengkap (RAKL) yang masing-masing terdiri dari 10 perlakuan dan 4 ulangan. Parameter yang diamati meliputi daya tumbuh F. oxysporum f.sp. zingiberi, gejala penyakit busuk rimpang, masa inkubasi, luas pembusukan dan selisih bobot basah rimpang. Hasil penelitian menunjukkan bahwa F. oxysporum f.sp. zingiberi yang telah di simpan 4 tahun dalam medium tanah steril mampu menyebabkan kerusakan pada rimpang dan tanaman jahe. Masa inkubasi gejala busuk pada rimpang serta gejala pada tanaman masing- masing berkisar antara 3–11,5 serta 55,5–68,5 hari. Isolat yang paling virulen adalah MSO1 dengan nilai luas pembusukan pada rimpang dan intensitas penyakit masing-masing sebesar 108,95 mm2 dan 33,88%.

Complete genome analysis of the novel Edwardsiella tarda phage vB_EtaM_ET-ABTNL-9

2020 ◽  
Vol 165 (5) ◽  
pp. 1241-1244
Author(s):  
Huijing Cui ◽  
Jiancheng Zhang ◽  
Cong Cong ◽  
Lili Wang ◽  
Xiaoyu Li ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Edwardsiella Tarda ◽  
The Novel ◽  
Complete Genome Analysis
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