Molecular characteristics and phylogenetic analysis of novel goose parvovirus strains associated with short beak and dwarfism syndrome

Abstract Short beak and dwarfism syndrome (SBDS) emerged in cherry valley duck flocks in China in 2015, and novel goose parvovirus (NGPV) was proved to be the etiological agent of SBDS. To date, whether SBDS-related NGPV isolates possess common molecular characteristics remains unknown. In this study, three new NGPV strains (namely, SDHT16, SDJN19, and SDLC19) were isolated from diseased ducks showing typical SBDS and successfully passaged in embryonated goose or cherry valley duck embryo. The whole genomes of three NGPV strains shared 98.9%–99.7% homologies between each other but showed slightly lower homologies (95.2%–96.1%) with the classical GPV strains. A total of 16 common amino acid point mutations were produced in the VP1 proteins of six NGPV strains (SDHT16, SDJN19, SDLC19, QH, JS1, and SDLC01) compared with the classical Chinese GPV strains, among which nine amino acid sites were identical to the European GPV strain B. The non-structural protein Rep1 of the six NGPV strains generated 12 common amino acid mutations compared with the classical GPV strains. The phylogenetic analysis indicated that the Chinese NGPV strains clustered with the European SBDS-related NGPV strains, forming a separate branch, distinct from the group formed by the classical GPV strains. Taken together, the present study unveils the common molecular characteristics of the NGPV isolates and directs the conclusion that the Chinese NGPV isolates probably originate from a common ancestor with the European SBDS-related NGPV.

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Genetic characterization and phylogenetic analysis of Novel Duck-Origin Goose Parvovirus in Anhui Province, Eastern China

10.21203/rs.3.rs-202531/v1 ◽

2021 ◽

Author(s):

Yong Wang ◽

Jianfei Sun ◽

Da Zhang ◽

Xu Guo ◽

Wenhao Shen ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genome Sequencing ◽

Phylogenetic Trees ◽

Genetic Characterization ◽

Eastern China ◽

Anhui Province ◽

Muscovy Duck ◽

Goose Parvovirus ◽

Short Beak ◽

Muscovy Duck Parvovirus

Abstract Recently, a novel duck-origin goose parvovirus (N-GPV) was reported to cause short beak and dwarfism syndrome in ducks. In this study, we performed complete genome sequencing and analyzed three different duck-derived parvoviruses that infected different breeds of ducks. Phylogenetic trees based on gene sequences indicated that they were classical goose parvovirus (C-GPV), Muscovy duck parvovirus (MDPV), and N-GPV, respectively. Furthermore, potential recombination events were found. These results improve our understanding of the diversity of duck-derived parvoviruses in the Anhui province, eastern China, and provide a reference for the prevention of associated diseases.

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Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China

Pathogens ◽

10.3390/pathogens10060711 ◽

2021 ◽

Vol 10 (6) ◽

pp. 711

Author(s):

Sixin Zhang ◽

Guangping Huang ◽

Liang Li ◽

Xianyong Liu ◽

Xiaoli Tang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Coi Gene ◽

Molecular Characteristics ◽

Mean Intensity ◽

Gnathostoma Spinigerum ◽

Zoonotic Parasite ◽

Third Stage ◽

Parasite Infections ◽

Asian Swamp Eel ◽

Swamp Eel

Nematode infections transmitted to humans by the consumption of wild or cultured eels are increasingly being reported. In the present study, 120 Asian swamp eel, Monopterus albus (Zuiew), individuals collected from China were examined for parasite infections, and 78 larval nematodes were isolated. Morphological and molecular characteristics, including sequence and phylogenetic analysis of the internal transcribed spacer (ITS) and cytochrome c oxidase subunit I (COI) gene regions, were employed to identify these nematodes at the lowest taxonomic level possible. Asian swamp eel was infected with two zoonotic parasite taxa: Gnathostoma spinigerum advanced third-stage larvae, with 6.67% prevalence and mean intensity = 1.25, and Eustrongylides sp. fourth-stage larvae, with 26.67% prevalence and mean intensity = 2.13. These findings evidence the need to enhance public hygiene and food safety awareness toward eel consumption

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Identification of a novel goose parvovirus (GPV) recombinant associated with short beak and dwarfism syndrome in Mainland China, 2015

Infection Genetics and Evolution ◽

10.1016/j.meegid.2016.04.013 ◽

2016 ◽

Vol 41 ◽

pp. 289-291 ◽

Cited By ~ 11

Author(s):

Shao Wang ◽

Xiao-xia Cheng ◽

Shi-long Chen ◽

Shi-feng Xiao ◽

Shao-ying Chen ◽

...

Keyword(s):

Mainland China ◽

Goose Parvovirus ◽

Short Beak

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Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

Advances in Virology ◽

10.1155/2019/7163747 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Gözde Erkiş-Güngör ◽

Bayram Çevik

Keyword(s):

Phylogenetic Analysis ◽

Citrus Tristeza Virus ◽

Molecular Characteristics ◽

Cloning And Sequencing ◽

Phylogenetic Groups ◽

The 1960S ◽

Tristeza Virus ◽

Virus Isolates ◽

Stem Pitting ◽

Citrus Tristeza

The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.

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Novel goose parvovirus in domestic Linwu sheldrakes with short beak and dwarfism syndrome, China

Transboundary and Emerging Diseases ◽

10.1111/tbed.13280 ◽

2019 ◽

Vol 66 (5) ◽

pp. 1834-1839 ◽

Cited By ~ 6

Author(s):

Chunhe Wan ◽

Rongchang Liu ◽

Cuiteng Chen ◽

Longfei Cheng ◽

Shaohua Shi ◽

...

Keyword(s):

Goose Parvovirus ◽

Short Beak

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Detection of Novel Goose Parvovirus Disease Associated with Short Beak and Dwarfism Syndrome in Commercial Ducks

Animals ◽

10.3390/ani10101833 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1833 ◽

Cited By ~ 1

Author(s):

Mohamed A. Soliman ◽

Ahmed M. Erfan ◽

Mohamed Samy ◽

Osama Mahana ◽

Soad A. Nasef

Keyword(s):

First Record ◽

Pekin Duck ◽

Economic Losses ◽

Morbidity Rate ◽

Muscovy Duck ◽

Performance Isolation ◽

Retarded Growth ◽

Goose Parvovirus ◽

Low Performance ◽

Short Beak

Derzsy’s disease causes disastrous losses in domestic waterfowl farms. A genetically variant strain of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) was named novel goose parvovirus (NGPV), which causes characteristic syndrome in young ducklings. The syndrome was clinically characterized by deformity in beaks and retarded growth, called short beaks and dwarfism syndrome (SBDS). Ten mule and pekin duck farms were investigated for parvovirus in three Egyptian provinces. Despite low recorded mortality rate (20%), morbidity rate was high (70%), but the economic losses were remarkable as a result of retarded growth and low performance. Isolation of NGPV was successful on primary cell culture of embryonated duck liver cells with a clear cytopathic effect. Partial gene sequence of the VP1 gene showed high amino acids identity among isolated strains and close identity with Chinese strains of NGPV, and low identity with classic GPV and MDPV strains. To the best of our knowledge, this can be considered the first record of NGPV infections in Egypt.

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Molecular characterization and phylogenetic analysis of Taenia multiceps from China

Acta Parasitologica ◽

10.1515/ap-2018-0085 ◽

2018 ◽

Vol 63 (4) ◽

pp. 721-727 ◽

Cited By ~ 1

Author(s):

L. Tan ◽

A.B. Wang ◽

S.Q. Zheng ◽

X.L. Zhang ◽

C.J. Huang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Dna Sequences ◽

Hunan Province ◽

Biological Study ◽

Economic Losses ◽

Molecular Characteristics ◽

12S Rrna ◽

Intermediate Hosts ◽

Taenia Multiceps ◽

Coenurus Cerebralis

Abstract Taenia multiceps, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of T.multiceps were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(pcox1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from T.multiceps and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between T.multiceps and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for pcox1, 12S rRNA and ITS, respectively, among T.multiceps and coenurus isolates obtained in this study. In Sichuan province, sequence variations for Coenurus cerebralis isolated from Yaan city were 0–1.4% for pcox1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of T.multiceps isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for pcox1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on pcox1 sequences indicated that all cerebral and noncerebral metacestodes belong to T.multiceps. These results provide reference values for future molecular epidemiological and biological study on T.multiceps in dogs and intermediate hosts.

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