Overexpression of AtEDT1 promotes root elongation and affects medicinal secondary metabolite biosynthesis in roots of transgenic Salvia miltiorrhiza

Salvia miltiorrhiza (Labiatae) is an important medicinal plant in traditional Chinese medicine. Tanshinones are one of the main active components of S. miltiorrhiza. It has been found that the intraspecific variation of S. miltiorrhiza is relatively large and the content of tanshinones in its roots of different varieties is also relatively different. To investigate the molecular mechanisms that responsible for the differences among these varieties, the tanshinones content was determined and comparative transcriptomics analysis was carried out during the tanshinones accumulation stage. A total of 52,216 unigenes were obtained from the transcriptome by RNA sequencing among which 23,369 genes were differentially expressed among different varieties, and 2,016 genes including 18 diterpenoid biosynthesis-related genes were differentially expressed during the tanshinones accumulation stage. Functional categorization of the differentially expressed genes (DEGs) among these varieties revealed that the pathway related to photosynthesis, oxidative phosphorylation, secondary metabolite biosynthesis, diterpenoid biosynthesis, terpenoid backbone biosynthesis, sesquiterpenoid and triterpenoid biosynthesis are the most differentially regulated processes in these varieties. The six tanshinone components in these varieties showed different dynamic changes in tanshinone accumulation stage. In addition, combined with the analysis of the dynamic changes, 277 DEGs (including one dehydrogenase, three CYP450 and 24 transcription factors belonging to 12 transcription factor families) related to the accumulation of tanshinones components were obtained. Furthermore, the KEGG pathway enrichment analysis of these 277 DEGs suggested that there might be an interconnection between the primary metabolic processes, signaling processes and the accumulation of tanshinones components. This study expands the vision of intraspecific variation and gene regulation mechanism of secondary metabolite biosynthesis pathways in medicinal plants from the “omics” perspective.

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0894 - Prokaryotic communities associated with Antarctic sponges and functional prediction of xenobiotic biodegradation and secondary metabolite biosynthesis

10.26226/morressier.5b5199c1b1b87b000ecee65d ◽

2018 ◽

Author(s):

Georg Steinert ◽

Dorte Janussen ◽

Thorsten Brinkhoff

Keyword(s):

Secondary Metabolite ◽

Functional Prediction ◽

Secondary Metabolite Biosynthesis ◽

Xenobiotic Biodegradation ◽

Antarctic Sponges

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Faculty Opinions recommendation of Novel soil bacteria possess diverse genes for secondary metabolite biosynthesis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733438832.793549082 ◽

2018 ◽

Author(s):

Kim Lewis ◽

Robert Green

Keyword(s):

Secondary Metabolite ◽

Soil Bacteria ◽

Secondary Metabolite Biosynthesis

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Regio- and stereoselective intermolecular phenol coupling enzymes in secondary metabolite biosynthesis

Natural Product Reports ◽

10.1039/d0np00010h ◽

2021 ◽

Author(s):

Wolfgang Hüttel ◽

Michael Müller

Keyword(s):

Secondary Metabolites ◽

Secondary Metabolite ◽

Metabolic Pathways ◽

Cyp Enzymes ◽

Secondary Metabolite Biosynthesis

Phenol coupling enzymes, especially laccases and CYP-enzymes create an enormous diversity of biarylic secondary metabolites in fungi, plants, and bacteria. The enzymes and the elucidation of the corresponding metabolic pathways are presented.

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Transcriptional Profiles of Secondary Metabolite Biosynthesis Genes and Cytochromes in the Leaves of Four Papaver Species

Data ◽

10.3390/data3040055 ◽

2018 ◽

Vol 3 (4) ◽

pp. 55 ◽

Cited By ~ 5

Author(s):

Dowan Kim ◽

Myunghee Jung ◽

In Ha ◽

Min Lee ◽

Seok-Geun Lee ◽

...

Keyword(s):

Secondary Metabolite ◽

Developmental Stages ◽

Expression Profiles ◽

Bioinformatics Analyses ◽

Kegg Pathways ◽

Benzylisoquinoline Alkaloid ◽

Development Life Cycle ◽

The Family ◽

Leaf Transcriptome ◽

Secondary Metabolite Biosynthesis

Poppies are well-known plants in the family Papaveraceae that are rich in alkaloids. This family contains 61 species, and in this study we sequenced the transcriptomes of four species’ (Papaver rhoeas, Papaver nudicaule, Papaver fauriei, and Papaver somniferum) leaves. These transcripts were systematically assessed for the expression of secondary metabolite biosynthesis (SMB) genes and cytochromes, and their expression profiles were assessed for use in bioinformatics analyses. This study contributed 265 Gb (13 libraries with three biological replicates) of leaf transcriptome data from three Papaver plant developmental stages. Sequenced transcripts were assembled into 815 Mb of contigs, including 226 Mb of full-length transcripts. The transcripts for 53 KEGG pathways, 55 cytochrome superfamilies, and benzylisoquinoline alkaloid biosynthesis (BIA) were identified and compared to four other alkaloid-rich genomes. Additionally, 22 different alkaloids and their relative expression profiles in three developmental stages of Papaver species were assessed by targeted metabolomics using LC-QTOF-MS/MS. Collectively, the results are given in co-occurrence heat-maps to help researchers obtain an overview of the transcripts and their differential expression in the Papaver development life cycle, particularly in leaves. Moreover, this dataset will be a valuable resource to derive hypotheses to mitigate an array of Papaver developmental and secondary metabolite biosynthesis issues in the future.

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