scholarly journals Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

2017 ◽  
Vol 36 (5) ◽  
pp. 519-528 ◽  
Author(s):  
Tomoyo Tanaka ◽  
Mitsuhiro Hoshijima ◽  
Junko Sunaga ◽  
Takashi Nishida ◽  
Mana Hashimoto ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Living Bone ◽  
Time Lapse Imaging

scholarly journals Time‐lapse imaging assay using the BioStation CT : A sensitive drug‐screening method for three‐dimensional cell culture

2015 ◽  
Vol 106 (6) ◽  
pp. 757-765 ◽  
Author(s):  
Ruriko Sakamoto ◽  
M. Mamunur Rahman ◽  
Manami Shimomura ◽  
Manabu Itoh ◽  
Tetsuya Nakatsura
Keyword(s):  
Cell Culture ◽  
Drug Screening ◽  
Screening Method ◽  
Three Dimensional ◽  
Time Lapse ◽  
Time Lapse Imaging ◽  
Dimensional Cell

Toxicity Evaluation of Two Dental Composites: Three-Dimensional Confocal Laser Scanning Microscopy Time-Lapse Imaging of Cell Behavior

2013 ◽  
Vol 19 (3) ◽  
pp. 596-607 ◽  
Author(s):  
Ghania Nina Attik ◽  
Nelly Pradelle-Plasse ◽  
Doris Campos ◽  
Pierre Colon ◽  
Brigitte Grosgogeat
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Cell Metabolism ◽  
Three Dimensional ◽  
Time Lapse ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Dental Composites ◽  
Confocal Laser ◽  
Time Lapse Imaging

AbstractThe purpose of this study was to investigate thein vitrobiocompatibility of two dental composites (namely A and B) with similar chemical composition used for direct restoration using three-dimensional confocal laser scanning microscopy (CLSM) time-lapse imaging. Time-lapse imaging was performed on cultured human HGF-1 fibroblast-like cells after staining using Live/Dead®. Image analysis showed a higher mortality rate in the presence of composite A than composite B. The viability rate decreased in a time-dependent manner during the 5 h of exposure. Morphological alterations were associated with toxic effects; cells were enlarged and more rounded in the presence of composite A as shown by F-actin and cell nuclei staining. Resazurin assay was used to confirm the active potential of composites in cell metabolism; results showed severe cytotoxic effects in the presence of both no light-curing composites after 24 h of direct contact. However, extracts of polymerized composites induced a moderate decrease in cell metabolism after the same incubation period. Composite B was significantly better tolerated than composite A at all investigated end points and all time points. The finding confirmed that the used CLSM method was sufficiently sensitive to differentiate the biocompatibility behavior of two composites based on similar methacrylate monomers.

Passive seismic tomography for three-dimensional time-lapse imaging of mining-induced rock mass changes

2012 ◽  
Vol 31 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Erik Westman ◽  
K. Luxbacher ◽  
S. Schafrik
Keyword(s):  
Rock Mass ◽  
Seismic Tomography ◽  
Three Dimensional ◽  
Time Lapse ◽  
Passive Seismic ◽  
Time Lapse Imaging

scholarly journals Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sangwoo Park ◽  
Lucy Eunju Lee ◽  
Hanna Kim ◽  
Ji Eun Kim ◽  
Seung Jun Lee ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Monosodium Urate ◽  
Monosodium Urate Crystals ◽  
Optical Diffraction Tomography ◽  
Time Lapse Imaging ◽  
Msu Crystals ◽  
Urate Crystals

AbstractOptical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.

scholarly journals Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jonathan M. Taylor ◽  
Carl J. Nelson ◽  
Finnius A. Bruton ◽  
Aryan Kaveh ◽  
Charlotte Buckley ◽  
...  
Keyword(s):  
Time Course ◽  
Cardiac Development ◽  
Three Dimensional ◽  
Time Lapse ◽  
Beating Heart ◽  
Cellular Mechanisms ◽  
Phase Lock ◽  
Optical Gating ◽  
Time Lapse Imaging ◽  
Zebrafish Heart

AbstractThree-dimensional fluorescence time-lapse imaging of the beating heart is extremely challenging, due to the heart’s constant motion and a need to avoid pharmacological or phototoxic damage. Although real-time triggered imaging can computationally “freeze” the heart for 3D imaging, no previous algorithm has been able to maintain phase-lock across developmental timescales. We report a new algorithm capable of maintaining day-long phase-lock, permitting routine acquisition of synchronised 3D + time video time-lapse datasets of the beating zebrafish heart. This approach has enabled us for the first time to directly observe detailed developmental and cellular processes in the beating heart, revealing the dynamics of the immune response to injury and witnessing intriguing proliferative events that challenge the established literature on cardiac trabeculation. Our approach opens up exciting new opportunities for direct time-lapse imaging studies over a 24-hour time course, to understand the cellular mechanisms underlying cardiac development, repair and regeneration.

Cleavage of Human Embryos: Options and Diversity

Acta Naturae ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 88-96
Author(s):  
Yu. K. Doronin ◽  
I. V. Senechkin ◽  
L. V. Hilkevich ◽  
M. A. Kurcer
Keyword(s):  
Time Lapse ◽  
Reproductive Technologies ◽  
Human Embryos ◽  
Imaging Data ◽  
Noninvasive Methods ◽  
Topographic Features ◽  
Time Parameters ◽  
Embryo Cleavage ◽  
Time Lapse Imaging ◽  
Developmental Dynamics

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

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