Force Modulation Elasticity Mapping of Plastic-embedded, Thin-sectioned Skeletal Muscle

2001 ◽  
Vol 7 (1) ◽  
pp. 32-38
Author(s):  
Boris B. Akhremitchev ◽  
Henry G. Brown ◽  
Scott R. Graner ◽  
Gilbert C. Walker
Keyword(s):  
Skeletal Muscle ◽  
Atomic Force Microscopy ◽  
Biological Material ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Force Microscopy ◽  
Force Modulation ◽  
Atomic Force ◽  
The Difference ◽  
Microscopic Techniques

AbstractWe have been researching the capability of atomic force microscopy to reveal nontopographic properties of tissue embedded in plastic and sectioned with standard electron microscopic techniques. We present topography and elasticity maps of plastic-embedded, thin sections of muscle tissue. The images show topography correlated with the normal repeating structure of the sarcomere. Elasticity mapping using force modulation revealed contrast between the actin- and myosin-rich areas. We attribute the observed contrast in elasticity to the difference in local concentrations of biological material in embedding plastic.

Investigation of surface morphology of thin metal films with magnetic layers Fe-Cr-Co

2021 ◽  
Author(s):  
V.S. Zayonchkovsky ◽  
Aung Kyaw Kyaw ◽  
A.V. Andreev
Keyword(s):  
Atomic Force Microscopy ◽  
Magnetron Sputtering ◽  
Electron Microscopic Examination ◽  
Nearest Neighbors ◽  
Substrate Plane ◽  
Large Area ◽  
Electron Microscopic ◽  
Force Microscopy ◽  
Atomic Force ◽  
Large Projection

Films containing layers of dispersion-hardening alloys (LDHA) based on the Fe-Cr-Co system were obtained by magnetron sputtering. LDHA acquire the properties of film permanent magnets after a single-stage «fast» high-vacuum annealing. Bulk materials acquire such properties only after many hours of multi-stage heat treatment. The film samples acquire these properties in tens of seconds. The morphology of their surface was studied to determine the origin of the coercive force of film samples. The surface morphology was studied using high resolution scanning electron microscopy and atomic force microscopy. We studied two compositions that, in bulk, have a different tendency to form many phases during crystallization. In magnetron sputtering, the alloy in which a multiphase state is easily formed is polycrystalline. The antipode alloy in magnetron sputtering is realized in an amorphous state. After annealing, both alloys are in a polycrystalline state. Electron microscopic examination showed that as a result of annealing, crystallites are formed with a large projection onto the substrate plane, which grow due to the nearest neighbors. Moreover, these crystallites have not only a large area, but also a height. After annealing, both alloys are in a polycrystalline state. Electron microscopic examination showed that as a result of annealing, crystallites are formed with a large projection onto the substrate plane, which grow due to the nearest neighbors. Moreover, these crystallites have not only a large area, but also a height. What is determined by atomic force microscopy. High crystallites are also faceted. This may indicate that the composition of these crystallites differs from the composition of the surrounding layer, which may be the reason for the increase in coercive force as a result of annealing.

Contact atomic force microscopy using piezoresistive cantilevers in load force modulation mode

2018 ◽  
Vol 184 ◽  
pp. 199-208 ◽  
Author(s):  
P. Biczysko ◽  
A. Dzierka ◽  
G. Jóźwiak ◽  
M. Rudek ◽  
T. Gotszalk ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Load Force ◽  
Force Microscopy ◽  
Force Modulation ◽  
Atomic Force ◽  
Modulation Mode ◽  
Piezoresistive Cantilevers

Growth of Double-Chained Cationic Surfactant Films on Mica

2006 ◽  
Vol 59 (6) ◽  
pp. 381 ◽  
Author(s):  
Annabelle Blom ◽  
Gregory G. Warr ◽  
Erica J. Wanless
Keyword(s):  
Atomic Force Microscopy ◽  
Alkyl Chain ◽  
Effect Of Temperature ◽  
Equilibration Time ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Difference ◽  
Chain Lengths ◽  
Gel Transition

The evolution of adsorbed dialkyl chained quaternary ammonium surfactant films with different alkyl chain lengths has been observed in situ using atomic force microscopy (AFM). Both di-C12DAB and di-C14DAB form a cohesive bilayer immediately, which is observed to strengthen with equilibration time. The slow equilibrium of di-C16DAB allows examination of the film at less than saturated coverage and reveals growth of the bilayer through the nucleation and coalescence of patches. The difference in height between higher and lower regions is insufficient for bilayer and monolayer regions and the postulated structure is that of regions of bilayer with different packing densities. The effect of temperature on film morphologies near the gel transition is also examined.

Imaging of reconstituted biological channels at molecular resolution by atomic force microscopy

1993 ◽  
Vol 265 (3) ◽  
pp. C851-C856 ◽  
Author(s):  
R. Lal ◽  
H. Kim ◽  
R. M. Garavito ◽  
M. F. Arnsdorf
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Internal Control ◽  
Imaging Method ◽  
Buffer Solution ◽  
Electron Microscopic ◽  
Ompf Porin ◽  
Force Microscopy ◽  
Atomic Force ◽  
Rectangular Pattern

Using atomic force microscopy (AFM), we obtained high-resolution surface images of the bacterial outer membrane channels Escherichia coli OmpF porin and Bordetella pertussis porin that were reconstituted in artificial bilayer membranes as two-dimensional crystalline arrays. These porins were chosen because they are among the most extensively studied proteins of this type and are known for their well-defined crystalline nature in the native membrane. Such reconstituted membrane proteins are ideal specimens to assess the suitability and resolution of AFM for imaging biomembranes and associated proteins. Although OmpF porin often showed a mixed pattern of rectangular and hexagonal arrays with approximately 8.4 x 9.8- and approximately 7.2-nm-spacings, respectively, B. pertussis porin showed mostly a rectangular pattern with an approximately 7.9 x 13.8-nm spacing. The packing patterns of the E. coli OmpF porin in the membrane are very close to those found in electron-microscopic studies. When B. pertussis porin was imaged in a buffer solution, its trimeric subunits were apparently resolved, and the surface of each monomer revealed beadlike structures. This is the first report of such a high-resolution structural analysis of B. pertussis porin by any imaging method. We also imaged the lipid bilayer itself as an internal control for imaging and to further ascertain the resolution. Individual polar head groups of bilayer lipid molecules were resolved, suggesting the intrinsic resolution of AFM for bioimaging.

scholarly journals Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

2016 ◽  
Vol 7 ◽  
pp. 220-227 ◽  
Author(s):  
Maryse D Nkoua Ngavouka ◽  
Pietro Capaldo ◽  
Elena Ambrosetti ◽  
Giacinto Scoles ◽  
Loredana Casalis ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Complementary Sequence ◽  
Single Base ◽  
Force Microscopy ◽  
Dna Monolayers ◽  
Atomic Force ◽  
The Difference

Background: DNA hybridization is at the basis of most current technologies for genotyping and sequencing, due to the unique properties of DNA base-pairing that guarantee a high grade of selectivity. Nonetheless the presence of single base mismatches or not perfectly matched sequences can affect the response of the devices and the major challenge is, nowadays, to distinguish a mismatch of a single base and, at the same time, unequivocally differentiate devices read-out of fully and partially matching sequences. Results: We present here two platforms based on different sensing strategies, to detect mismatched and/or perfectly matched complementary DNA strands hybridization into ssDNA oligonucleotide monolayers. The first platform exploits atomic force microscopy-based nanolithography to create ssDNA nano-arrays on gold surfaces. AFM topography measurements then monitor the variation of height of the nanostructures upon biorecognition and then follow annealing at different temperatures. This strategy allowed us to clearly detect the presence of mismatches. The second strategy exploits the change in capacitance at the interface between an ssDNA-functionalized gold electrode and the solution due to the hybridization process in a miniaturized electrochemical cell. Through electrochemical impedance spectroscopy measurements on extended ssDNA self-assembled monolayers we followed in real-time the variation of capacitance, being able to distinguish, through the difference in hybridization kinetics, not only the presence of single, double or triple mismatches in the complementary sequence, but also the position of the mismatched base pair with respect to the electrode surface. Conclusion: We demonstrate here two platforms based on different sensing strategies as sensitive and selective tools to discriminate mismatches. Our assays are ready for parallelization and can be used in the detection and quantification of single nucleotide mismatches in microRNAs or in genomic DNA.

Analysis of Intra-Sarcomere Stractures of Skeletal Muscle Myofibrils by use of Atomic Force Microscopy

2001 ◽  
Vol 41 (supplement) ◽  
pp. S53
Author(s):  
M. Aimi ◽  
Y. Kunioka ◽  
T. Yamada
Keyword(s):  
Skeletal Muscle ◽  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Atomic Force
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