Abstract
Background: Stem cell transplantation has been proposed as an alternative treatment for intractable optic nerve disorders characterized by irrecoverable loss of cells. Mesenchymal stem cells (MSCs), with varying tissue regeneration and recovery capabilities, are being considered for potential cell therapies. To overcome the limitations of cell therapy, we isolated exosomes from human placenta–derived mesenchymal stem cells (hPMSCs), and investigated their therapeutic effects in R28 cells (retinal precursor cells) exposed to CoCl2 . Method: We investigated recovery effects of exosomes in vitro damaged cells. We exposed R28 cells to CoCl2. After 9 h, the hypoxia-damaged R28 cells were treated with hPMSC-derived exosomes. We examined the changes in the target proteins of R28 cells and performed proteomic analysis using R28 cells. Result: Upon this exosome treatment of R28 cells damaged by a hypoxic environment, the expression of Hif-1α protein (which increased after CoCl2 exposure) significantly decreased, whereas that of nerve regeneration–related factors such as Thy-1 and Neuroflament (which decreased after CoCl2 exposure) significantly increased. Proteomic analysis was used to analyze the expression of candidate target proteins such as UBA2 and catenin, which showed significant changes in expression during the recovery period in the damaged cell group. Conclusion: In this study, we discovered that UBA2 played a key role in activating the Wnt/β-catenin signaling pathway during the recovery process of damaged R28 cells, induced by hPMSC exosomes.
Identification of the target proteins of rosiglitazone in 3T3-L1 adipocytes through proteomic analysis of cytosolic and secreted proteins
