Monitoring of genetically modified food and feed in the Tunisian market using qualitative and quantitative real-time PCR

2013 ◽  
Vol 22 (4) ◽  
pp. 1161-1170 ◽  
Author(s):  
Maher Chaouachi ◽  
Nesrine Nabi ◽  
Ahmed Ben Hafsa ◽  
Mohamed Salem Zellama ◽  
Fethia Skhiri ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Food ◽  
Quantitative Real Time Pcr ◽  
Qualitative And Quantitative ◽  
Food And Feed

Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection

2013 ◽  
Vol 61 (25) ◽  
pp. 5953-5960 ◽  
Author(s):  
Yuhua Wu ◽  
Litao Yang ◽  
Yinglong Cao ◽  
Guiwen Song ◽  
Ping Shen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Quantitative Real Time Pcr ◽  
Genetically Modified Rice ◽  
Pcr Method

scholarly journals Verification of Real-Time PCR Methods for Qualitative and Quantitative Testing of Genetically Modified Organisms

2012 ◽  
Vol 35 (6) ◽  
pp. 442-447 ◽  
Author(s):  
S. Del Gaudio ◽  
A. Cirillo ◽  
G. Di Bernardo ◽  
U. Galderisi ◽  
M. Cipollaro
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Qualitative And Quantitative

Molecular Identification of Four Genetically Modified Maize (Bt11, Bt176, Mon810 and T25) by Duplex Quantitative Real-Time PCR

2013 ◽  
Vol 7 (1) ◽  
pp. 224-233 ◽  
Author(s):  
Maher Chaouachi ◽  
Mohamed Salem Zellama ◽  
Nesrine Nabi ◽  
Ahmed Ben Hafsa ◽  
Khaled Saïd
Keyword(s):  
Real Time ◽  
Molecular Identification ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Quantitative Real Time Pcr

Development of an Event-Specific Detection Method for Genetically Modified Maize IE034 by Quantitative Real-Time PCR

2016 ◽  
Vol 10 (6) ◽  
pp. 1727-1735
Author(s):  
Feiwu Li ◽  
Likun Long ◽  
Wei Yan ◽  
Congcong Li ◽  
Junfeng Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Method ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Quantitative Real Time Pcr ◽  
Specific Detection

Detection of Genetically Modified Maize MON88017 by Quantitative Real- time PCR

2012 ◽  
Vol 37 (11) ◽  
pp. 2117-2121
Author(s):  
Lei YUAN ◽  
Hong-Wei SUN ◽  
Fan LI ◽  
Ning LI ◽  
Lei ZHAO ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Quantitative Real Time Pcr

scholarly journals KLK11 promotes the activation of mTOR and protein synthesis to facilitate cardiac hypertrophy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yi Wang ◽  
Hongjuan Liao ◽  
Yueheng Wang ◽  
Jinlin Zhou ◽  
Feng Wang ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Angiotensin Ii ◽  
Cardiac Hypertrophy ◽  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Mtor Signaling ◽  
Heart Weight ◽  
Cardiomyocyte Hypertrophy ◽  
Quantitative Real Time Pcr

Abstract Background Cardiovascular diseases have become the leading cause of death worldwide, and cardiac hypertrophy is the core mechanism underlying cardiac defect and heart failure. However, the underlying mechanisms of cardiac hypertrophy are not fully understood. Here we investigated the roles of Kallikrein 11 (KLK11) in cardiac hypertrophy. Methods Human and mouse hypertrophic heart tissues were used to determine the expression of KLK11 with quantitative real-time PCR and western blot. Mouse cardiac hypertrophy was induced by transverse aortic constriction (TAC), and cardiomyocyte hypertrophy was induced by angiotensin II. Cardiac function was analyzed by echocardiography. The signaling pathway was analyzed by western blot. Protein synthesis was monitored by the incorporation of [3H]-leucine. Gene expression was analyzed by quantitative real-time PCR. Results The mRNA and protein levels of KLK11 were upregulated in human hypertrophic hearts. We also induced cardiac hypertrophy in mice and observed the upregulation of KLK11 in hypertrophic hearts. Our in vitro experiments demonstrated that KLK11 overexpression promoted whereas KLK11 knockdown repressed cardiomyocytes hypertrophy induced by angiotensin II, as evidenced by cardiomyocyte size and the expression of hypertrophy-related fetal genes. Besides, we knocked down KLK11 expression in mouse hearts with adeno-associated virus 9. Knockdown of KLK11 in mouse hearts inhibited TAC-induced decline in fraction shortening and ejection fraction, reduced the increase in heart weight, cardiomyocyte size, and expression of hypertrophic fetal genes. We also observed that KLK11 promoted protein synthesis, the key feature of cardiomyocyte hypertrophy, by regulating the pivotal machines S6K1 and 4EBP1. Mechanism study demonstrated that KLK11 promoted the activation of AKT-mTOR signaling to promote S6K1 and 4EBP1 pathway and protein synthesis. Repression of mTOR with rapamycin blocked the effects of KLK11 on S6K1 and 4EBP1 as well as protein synthesis. Besides, rapamycin treatment blocked the roles of KLK11 in the regulation of cardiomyocyte hypertrophy. Conclusions Our findings demonstrated that KLK11 promoted cardiomyocyte hypertrophy by activating AKT-mTOR signaling to promote protein synthesis.

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