Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk

2015 ◽  
Vol 79 (17-18) ◽  
pp. 1091-1100 ◽  
Author(s):  
Nicolás Michlig ◽  
María Rosa Repetti ◽  
Carolina Chiericatti ◽  
Silvia R. García ◽  
Mónica Gaggiotti ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Raw Milk ◽  
Aflatoxin M1

Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton

2020 ◽  
pp. 260-267
Author(s):  
Huska Jukić ◽  
Samira Dedić ◽  
Miloš Rodić ◽  
Zlatko Jusufhodžić ◽  
Dinko Demirović
Keyword(s):  
Raw Milk ◽  
Aflatoxin M1 ◽  
Elisa Method

A survey on the occurrence of aflatoxin M1 in raw milk samples in Bafq and Bahabad, Iran

2021 ◽  
Author(s):  
Vahid Safavizadeh ◽  
Mozhgan Mojkar
Keyword(s):  
Aspergillus Parasiticus ◽  
Raw Milk ◽  
National Standard ◽  
Aflatoxin M1 ◽  
Cow’S Milk ◽  
Elisa Method ◽  
Cow's Milk ◽  
Milk Samples ◽  
The City

Aflatoxins are a group of mycotoxins mostly produced by the fungi called Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomium. Aflatoxin M1 (AFM1) is the major metabolite of aflatoxin B1 and is a hepatotoxic and carcinogenic toxin. The aim of this study was to determine the level of contamination of cow's milk with aflatoxin M1 in Bafq and Bahabad. For this study, samples of raw cow's milk were collected randomly from milk collection centers around the city of Bafq and Bahabad from March to April. The determination of aflatoxin M1 levels was based on the ELISA method. Contamination was observed in 100% of milk samples. According to the results of the study, the rate of contamination with aflatoxin M1 in 43.3% of milk samples was above the acceptable level (50 ng/L) in Iranian national standard. It is concluded that further monitoring of milk production should be carried out in the spring and winter seasons.

scholarly journals Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

2020 ◽  
pp. 150-157
Author(s):  
S. Senin ◽  
V. Danchuk ◽  
S. Midyk ◽  
V. Ushkalov ◽  
O. Iakubchak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Analytical Methods ◽  
Dairy Products ◽  
High Performance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk And Dairy Products

The dairy industry of Ukraine is developing dynamically, its needs for the quality of raw materials are growing significantly. Detection of mycotoxins in raw milk is one of the main indicators of its safety. The high degree of toxicity of mycotoxins is a threat to the health of the lactating animal, so a large number of them are excreted in milk. If we talk about ruminants, the vast majority of mycotoxins are utilized by microorganisms of the pancreas, which does not occur in monogastric animals, so the list of mycotoxins in their milk can be much wider than the secretion of mammalian mammals. To date, the maximum permissible levels (MRLs) of mycotoxins in raw milk and dairy products have been established. Thus, a comprehensive determination of the content of mycotoxins in the secretion of the breast has not only technological but also important diagnostic value. Milk sample preparation is the most important step in the determination of mycotoxins and consists of sampling, extraction and purification from impurities. For the extraction of aflatoxins, the method of liquid extraction with acetonitrile or chloroform is used. Purification of extracts is carried out on immunoaffinity columns, cartridges with special sorbents or using certain manufacturers (MycoSep®).Enzyme-linked immunosorbent assay and high-performance liquid chromatography with fluorescence detection are used to determine aflatoxin B1 and M1 in raw milk of cows. However, all these methods have a number of disadvantages, namely: long and expensive sample preparation and insufficiently high selectivity. Currently, the complex determination of mycotoxins in various matrices by high-performance liquid chromatography with mass spectrometric detection (LC-MS/MS) and the use of modified QuEChERS sample preparation is gaining popularity. The advantage of this technique is the combination of faster and cheaper sample preparation of QuEChERS samples with highly selective LC-MS/MS chromatography. Key words: mycotoxins, raw milk, analytical methods, QuEChERS.

Determination of aflatoxin M1 levels in raw milk samples in Gilan, Iran

2013 ◽  
Vol 5 ◽  
pp. 151-156 ◽  
Author(s):  
M. Laleh rokhi ◽  
R. Kazemi Darsanaki ◽  
M. Mohammadi ◽  
M. Hassani Kolavani ◽  
Kh. Issazadeh ◽  
...  
Keyword(s):  
Raw Milk ◽  
Aflatoxin M1 ◽  
Milk Samples

scholarly journals Determination of Aflatoxin M1 in Raw Milk Using an HPLC-FL Method in Comparison with Commercial ELISA Kits—Application in Raw Milk Samples from Various Regions of Greece

2021 ◽  
Vol 8 (3) ◽  
pp. 46
Author(s):  
Martha Maggira ◽  
Maria Ioannidou ◽  
Ioannis Sakaridis ◽  
Georgios Samouris
Keyword(s):  
Limit Of Detection ◽  
Reference Method ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescence Detector ◽  
Elisa Kit ◽  
Milk Samples ◽  
Of Greece

The highly toxic Aflatoxin M1 (AFM1) is most often detected in milk using an Enzyme-Linked-Immunosorbent Assay (ELISA) for screening purposes, while High-Performance Liquid Chromatography with Fluorescence Detector (HPLC-FL) is the reference method used for confirmation. The aim of the present study was the comparison between three commercially available ELISA kits and a newly developed HPLC-FL method for the determination of the AFM1 in milk samples. The developed HPLC-FL method was validated for the AFM1 and Aflatoxin M2 (AFM2), determining the accuracy, precision, linearity, decision limit, and detection capability with fairly good results. All three ELISA kits were also validated and showed equally good performance with high recovery rates. Moreover, the Limit Of Detection (LOD) and Limit Of Quantification (LOQ) values were found to be significantly lower than the Maximum Residue Limit (MRL) (50 ng kg−1). After the evaluation of all three commercial kits, the ELISA kit with the optimum performance along with the HPLC method was used for the determination of AFM1 in raw cow’s, goat’s, and sheep’s milk samples (396) obtained from producers in different regions of Greece. The evaluation of both methods showed that this ELISA kit could be considered as a faster and equally reliable alternative method to HPLC in routine analysis for the determination of AFM1 in milk.

scholarly journals Reduction of Aflatoxin M1 Levels during Ethiopian Traditional Fermented Milk (Ergo) Production

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Tsige Shigute ◽  
Alemayehu P. Washe
Keyword(s):  
Incubation Time ◽  
Raw Milk ◽  
Fermented Milk ◽  
Aflatoxin M1 ◽  
Chemical Components ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Cells ◽  
Milk Samples ◽  
Significant Difference

In this study, the reduction of aflatoxin M1 (AFM1) levels during lab-scale ergo production was investigated through determination of the residual levels of AFM1 using Enzyme Linked Immunosorbent Assay. The results showed gradual and incubation time dependent reduction of AFM1 level in the raw milk samples being fermented to ergo. The maximum reductions of 57.33 and 54.04% were recorded in AFM1 in natural and LAB inoculums initiated fermentations, respectively, in 5 days of incubation. Although a significant difference (P=0.05) in the AFM1 decrease in the two types of fermentations was recorded, such findings could vary with milk samples depending on initial load of the microorganisms as determined by hygienic conditions. However, the level of AFM1 in control (sterilized) samples showed only a 5.5% decrease during the entire period of incubation. Microbiological investigation showed increasing LAB counts with incubation time. A gradual decrease in pH of the milk samples was observed during fermentation. Considering the fact that both viable and dead bacterial cells could remove AFM1 during ergo production, the mechanism is proposed as predominantly involving noncovalent binding of the toxin with the chemical components of the bacterial cell wall.

Fully automated column-switching HPLC determination of aflatoxin M1 in milk using dialysis as sample preparation

1989 ◽  
Vol 12 (11) ◽  
pp. 709-713 ◽  
Author(s):  
L. G. M. Th. Tuinstra ◽  
P. G. M. Kienhuis ◽  
W. A. Traag ◽  
M. M. L. Aerts ◽  
W. M. J. Beek
Keyword(s):  
Sample Preparation ◽  
Column Switching ◽  
Hplc Determination ◽  
Aflatoxin M1
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