In vitro compatibility of Pimpinella anisum and Origanum vulgare essential oils with nematophagous fungi and their effects against Nacobbus aberrans

AbstractPhytopathogenic fungi have been responsible for considerable economic losses in vineyards, and therefore, more attention should be paid to the development and implementation of preventative treatment that is environmentally friendly. The aim of this study was to evaluate the antifungal activity of ten essential oils (EOs) (viz. Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.). For the antifungal activity evaluation against Penicillium brevicompactum, P. citrinum, P. crustosum, P. expansum, P. funiculosum, P. glabrum, P. chrysogenum, P. oxalicum, P. polonicum and Talaromyces purpurogenus a disc diffusion method was used. The ten EOs exhibited different antifungal properties. Three tested EOs (Carum carvi L., Satureja hortensis L. and Pimpinella anisum L.) at concentrations of 0.75, 0.50, 0.25 and 0.125 µL/mL showed antifungal activity, inhibiting the mycelial growth. The Origanum vulgare L. EOs exhibited a lower level of inhibition. Overall, Lavandula angustifolia Mill., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Pimpinella anisum L. and Rosmarinus officinalis L. were effective as fungicidal agents but their efficiency varied between the strains of fungi. Carum carvi L. showed strong antifungal activity against all tested strains at both full strength and reduced concentrations. These EOs could be considered as potential sources of antifungal compounds for treating plant fungal diseases.

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In vitro activity of the essential oils of Origanum vulgare, Satureja montana and their main constituents in peroxynitrite-induced oxidative processes

Food Chemistry ◽

10.1016/j.foodchem.2006.10.064 ◽

2007 ◽

Vol 104 (3) ◽

pp. 889-895 ◽

Cited By ~ 53

Author(s):

Josè M. Prieto ◽

Patrizia Iacopini ◽

Pierluigi Cioni ◽

Silvio Chericoni

Keyword(s):

Essential Oils ◽

Vitro Activity ◽

In Vitro Activity ◽

Origanum Vulgare ◽

Oxidative Processes ◽

Satureja Montana

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Essential oils against Candida spp: in vitro antifungal activity of Origanum vulgare

African Journal of Microbiology Research ◽

10.5897/ajmr12.1149 ◽

2013 ◽

Vol 7 (20) ◽

pp. 2245-2250 ◽

Cited By ~ 6

Author(s):

Brum Cleff Marlete ◽

Madrid Isabel ◽

Raquel Meinerz Ana ◽

Carlos Arauacute jo Meireles Maacute rio ◽

Roberto Braga de Mello Joatilde o ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Origanum Vulgare ◽

Candida Spp ◽

In Vitro Antifungal Activity

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In vitro synergic efficacy of the combination of Nystatin with the essential oils of Origanum vulgare and Pelargonium graveolens against some Candida species

Phytomedicine ◽

10.1016/j.phymed.2009.02.011 ◽

2009 ◽

Vol 16 (10) ◽

pp. 972-975 ◽

Cited By ~ 37

Author(s):

Antonio Rosato ◽

Cesare Vitali ◽

Monica Piarulli ◽

Manuela Mazzotta ◽

Maria Pia Argentieri ◽

...

Keyword(s):

Essential Oils ◽

Candida Species ◽

Origanum Vulgare ◽

Pelargonium Graveolens

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Corrigendum: Stojkovic D, Glamoclija J, Ciric A, Nikolic M, Ristic M, Siljegovic J, Sokovic M. Investigation on antibacterial synergism of Origanum vulgare and Thymus vulgaris essential oils. Arch biol sci. 2013;65(2):639-43. DOI: 10.2298/ABS1302639S

Archives of Biological Sciences ◽

10.2298/abs150713085e ◽

2015 ◽

Vol 67 (4) ◽

pp. 1441-1441

Author(s):

E Editorial

Keyword(s):

Essential Oils ◽

In Vitro Model ◽

Medicinal Herbs ◽

Thymus Vulgaris ◽

Antibacterial Effects ◽

Origanum Vulgare ◽

Vitro Model ◽

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The authors of the above-referenced article have requested a corrigendum to it. The article should include the following reference: Sokovic, M., Glamoclija, J., Marin, D.P., Brkic, D., and L. J. L. D. van Griensven (2010) Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules. 15(11), 7532-7546. Link to the corrected article <a href="http://dx.doi.org/10.2298/ABS1302639S">10.2298/ABS1302639S</a>

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In vitro Inhibitory Action of the Essential Oils of Origanum Vulgare and Rosmarinus Officinalis against Aspergillus Fumigatus

Planta Medica International Open ◽

10.1055/a-1588-2875 ◽

2021 ◽

Vol 8 (03) ◽

pp. e143-e152

Author(s):

Antonia Carolina Melo Monteiro ◽

Aminata Doucoure Drame ◽

Francisca Melo Nascimento ◽

Ana Luisa Miranda-Vilela ◽

Alexandre Vasconcelos Lima ◽

...

Keyword(s):

Essential Oils ◽

Aspergillus Fumigatus ◽

Inhibitory Action ◽

Rosmarinus Officinalis ◽

Disk Diffusion ◽

In Vivo Studies ◽

Origanum Vulgare ◽

Agar Dilution

Abstract Aspergillus fumigatus is the main etiological agent of aspergillosis. Considering azole antifungal drug resistance in A. fumigatus, which compromises treatment, new alternatives are needed. Among them, essential oils (EOs) can be an alternative treatment, having shown positive results in inhibiting phytopathogenic fungi in vitro. We aimed to determine the in vitro antifungal activity of Origanum vulgare L. subsp. hirtum (Link) (oregano) and Rosmarinus officinalis L. (rosemary) EOs alone and in association (O. vulgare+R. officinalis) against A. fumigatus. EOs were analyzed by gas chromatography (GC-FID and GC/MS systems), and analyses showed that the major components of O. vulgare EO were carvacrol (67.8%), p-cymene (14.8%), and thymol (3.9%); for R. officinalis, they were the monoterpenes 1,8-cineole (49.1%), camphor (18.1%) and α-pinene (8.1). For biological assays, five EO concentrations, 0.2; 0.4; 0.6; 0.8 and 1.0%, were used in disk diffusion and agar dilution tests for 21 days. In disk diffusion, O. vulgare EO alone and in association (O. vulgare+R. officinalis) showed fungicidal activity at all concentrations. In agar dilution, inhibitory action was demonstrated from 0.6% for O. vulgare EO and in association (O. vulgare+R. officinalis). R. officinalis EO at 1.0% showed no fungal growth, determining the minimum inhibitory concentration (MIC). The present study demonstrated inhibitory actions of O. vulgare and R. officinalis EOs in A. fumigatus. GC analyses corroborated the literature regarding their antibacterial and antifungal effects. However, further in vitro and in vivo studies are needed to evaluate EOs as alternative antifungals for treating aspergillosis.

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New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives

Antibiotics ◽

10.3390/antibiotics10121460 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1460

Author(s):

Zorana Kovačević ◽

Nebojša Kladar ◽

Ivana Čabarkapa ◽

Miodrag Radinović ◽

Milan Maletić ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Antibacterial Properties ◽

Subclinical Mastitis ◽

Antioxidant Potential ◽

In Vitro Assays ◽

Origanum Vulgare ◽

Milk Samples ◽

Satureja Montana

Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.

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Evaluation of Plant Origin Essential Oils as Herbal Biocides for the Protection of Caves Belonging to Natural and Cultural Heritage Sites

Microorganisms ◽

10.3390/microorganisms9091836 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1836

Author(s):

Anthoula A. Argyri ◽

Agapi I. Doulgeraki ◽

Eftychia G. Varla ◽

Vasiliki C. Bikouli ◽

Pantelis I. Natskoulis ◽

...

Keyword(s):

Cultural Heritage ◽

Essential Oils ◽

Inhibitory Concentration ◽

Plant Origin ◽

Bacterial Isolates ◽

Origanum Vulgare ◽

Heritage Sites ◽

Fungal Isolates ◽

Satureja Thymbra

The present study concerns the serious issue of biodeterioration of the caves belonging to natural and cultural heritage sites due to the development of various microorganisms. Thus, a series of 18 essential oils (EOs) extracted from various Greek plants were evaluated in vitro (concentrations of 0.1, 0.2, 0.5, 1.0 and 5.0% v/v) against 35 bacterial and 31 fungi isolates (isolated from a Greek cave) and the antimicrobial activity was evident through the changes in optical density of microbial suspensions. In continuance, eight (8) representative bacterial and fungal isolates were further used to evaluate the minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values of the most effective EOs. According to the results, two EOs of Origanum vulgare were the most effective by inhibiting the growth of all the tested microorganisms at 0.1% (v/v), followed by that of Satureja thymbra which inhibited all bacterial isolates at 0.1% (v/v) and fungal isolates at 0.1, 0.2 and 0.5% (v/v) (depending on the isolate). The MIC ranged between 0.015–0.157 and 0.013–0.156 (v/v) for the bacterial and fungal isolates respectively, depending on the case. The current study demonstrated that conventional biocides may be replaced by herbal biocides with significant prospects for commercial exploitation.

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