The Biological Control of Fusarium oxysporum, the Causal Agent of Potato Rot

2022 ◽  
Author(s):  
Gözdenur Çakar ◽  
Elif Tozlu
Author(s):  
Tania Ameyally Rios-Hernández ◽  
Alberto Uc-Varguez ◽  
Zahaed Evangelista-Martínez

<em>Fusarium oxysporum</em> causa la pudrición del cormo en gladiolo provocando pérdidas de hasta el 100%. Se seleccionaron aislados de Fusarium a partir de cormos infectados, se identificó morfológica y molecularmente y se seleccionó un aislado a partir de prueba de patogenicidad. Se seleccionó entre 22 aislados de estreptomicetos una cepa que presentó una actividad antagonista del 40% contra <em>Fusarium</em>. Se obtuvo el Extracto Bioactivo (EB) mediante Fermentación en Fase Sólida y se determinó la concentración mínima inhibitoria (MIC) y concentración mínima letal (MLC) por el método de microdilución. Se obtuvo una MIC para el EB de 0.19 mg mL-1 y una MLC de 0.38 mg mL-1, que se confirmó con un ensayo de germinación de microconidios a 8 h, mostrando un porcentaje de inhibición del 17 y 98% para ¼ y ½ de la MIC. Se evaluó el efecto del EB a 1 y 2 MIC’s de concentración contra la pudrición en cormos de gladiolo infectados, obteniendo un efecto protector en los cormos al mantener su dureza después de 15 días, en comparación con el fungicida Carbendazim. Los resultados indican a <em>Streptomyces</em> sp., como un potencial agente de control biológico contra <em>F. oxysporum</em>.


2005 ◽  
Vol 1 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Sara Shafii Baftii ◽  
Gholam Hosein Shahid Bonjar ◽  
Sonia Aghighi ◽  
Sara Biglari ◽  
Parvin Rashid Farrokhi ◽  
...  

1987 ◽  
Vol 33 (5) ◽  
pp. 349-353 ◽  
Author(s):  
T. C. Paulitz ◽  
C. S. Park ◽  
R. Baker

Nonpathogenic isolates of Fusarium oxysporum were obtained from surface-disinfested, symptomless cucumber roots grown in two raw (nonautoclaved) soils. These isolates were screened for pathogenicity and biological control activity against Fusarium wilt of cucumber in raw soil infested with Fusarium oxysporum f. sp. cucumerinum (F.o.c.). The influence of three isolates effective in inducing suppressiveness and three ineffective isolates on disease incidence over time was tested. The effective isolates reduced the infection rate (R), based on linear regressions of data transformed to loge (1/1 – y). Effective isolate C5 was added to raw soil infested with various inoculum densities of F.o.c. In treatments without C5, the increase in inoculum densities of F.o.c. decreased the incubation period of wilt disease, but there was no significant difference in infection rate among the inoculum density treatments. Isolate C5 reduced the infection rate at all inoculum densities of F.o.c. Various inoculum densities of C5 were added to raw soils infested with 1000 cfu/g of F.o.c. In the first trial, infection rates were reduced only in the treatment with 10 000 cfu/g of C5; in the second trial, infection rates were reduced in treatments with 10 000 and 30 000 cfu/g of C5.


Author(s):  
Castañeda Alvarez Estefania ◽  
Sánchez Leal Ligia

For farmers the use of agrochemicals is the preferred method to control pests and diseases. Considering the market demand for biological control products, the encapsulation could be a competent alternative to current commercial formulations for cellular viability and controlled release. The purpose of this study was to use ionic gelation with sodium alginate, starch and maltodextrin to immobilize Bacillus subtilis and to evaluate the biocontrol effect against Fusarium oxysporum f. sp. lycopersici in vitro. The matrix with a concentration of 2% sodium alginate, 1% starch, and 1% maltodextrin is a suitable method for cellular viability and biological control activity against Fusarium oxysporum f. sp. lycopersici, with a reduction of mycelial growth of 49.6% and a survival rate for Bacillus subtilis of 98.05% (p less than 0.0001).The use of immobilized bacteria as biological control agents are sustainable and effective bio-inputs that could be used at industrial scale and benefit the tomato crops against attack by Fusarium oxysporum f. sp. lycopersici.


2014 ◽  
Vol 14 (2) ◽  
pp. 142-151
Author(s):  
I Ketut Suada ◽  
Ni Wayan Suniti

Isolation and identification of mangosteen yellow latex pathogen through Koch’s Postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease caused phusilogical disorder so that cells break due to unstability of cell turgor, the drift irrigation treatment was able to decrease the disease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogens with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species.  The result of the research proved that there were three fungus species as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity ( 74.87%), followed by Fusarium oxysporum ( 70.15%), and Pestalotia macrotricha (20.32%).


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