scholarly journals Initial Fiber Alignment Pattern Alters Extracellular Matrix Synthesis in Fibroblast-Populated Fibrin Gel Cruciforms and Correlates with Predicted Tension

2010 ◽  
Vol 39 (2) ◽  
pp. 714-729 ◽  
Author(s):  
E. A. Sander ◽  
V. H. Barocas ◽  
R. T. Tranquillo
Keyword(s):  
Extracellular Matrix ◽  
Matrix Synthesis ◽  
Fibrin Gel ◽  
Fiber Alignment ◽  
Initial Fiber

scholarly journals Extracellular matrix synthesis by skeletal muscle in culture. Proteins and effect of enzyme degradation.

1982 ◽  
Vol 257 (19) ◽  
pp. 11437-11442
Author(s):  
R L Beach ◽  
W V Burton ◽  
W J Hendricks ◽  
B W Festoff
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Matrix Synthesis ◽  
Enzyme Degradation

Cultured hepatocytes are contaminated by Ito cells: Relevance for studies of extracellular matrix synthesis

1989 ◽  
Vol 9 ◽  
pp. S182
Author(s):  
M. Lenzi ◽  
R. Solmi ◽  
G. Ballardini ◽  
P. Croff ◽  
F. Giostra ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cultured Hepatocytes ◽  
Matrix Synthesis ◽  
Ito Cells

scholarly journals Cyclosporin A affects extracellular matrix synthesis and degradation by mouse MC3T3‐E1 osteoblasts in vitro

2001 ◽  
Vol 16 (3) ◽  
pp. 500-505 ◽  
Author(s):  
Alessia Fornoni ◽  
Flavia Cornacchia ◽  
Guy A. Howard ◽  
Bernard A. Roos ◽  
Gary E. Striker ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cyclosporin A ◽  
Matrix Synthesis ◽  
Synthesis And Degradation

Gene expression, cytoskeletal changes and extracellular matrix synthesis in human osteoblasts treated with cyclosporin A

2009 ◽  
Vol 63 (9) ◽  
pp. 619-626 ◽  
Author(s):  
Maurizio Vertemati ◽  
Ernesto Minola ◽  
Claudia Dolci ◽  
Giordano Stabellini ◽  
Furio Pezzetti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Cyclosporin A ◽  
Matrix Synthesis ◽  
Human Osteoblasts

scholarly journals Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties

2016 ◽  
Vol Volume 9 ◽  
pp. 461-472 ◽  
Author(s):  
Hanane Chajra ◽  
Daniel Auriol ◽  
Francine Joly ◽  
Aurélie Pagnon ◽  
Magda Rodrigues ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Human Skin ◽  
Matrix Synthesis

Extracellular matrix synthesis in the chick embryo lateral plate prior to and during limb outgrowth

Development ◽  
1980 ◽  
Vol 59 (1) ◽  
pp. 71-87
Author(s):  
Trent D. Stephens ◽  
N. S. Vasan ◽  
James W. Lash
Keyword(s):  
Extracellular Matrix ◽  
Chick Embryo ◽  
Molecular Basis ◽  
Limb Bud ◽  
Matrix Synthesis ◽  
Lateral Plate ◽  
Limb Buds ◽  
Cartilage Development ◽  
Limb Outgrowth ◽  
Lateral Plates

Little is known at the present time about the molecular basis and mechanisms of morphogenesis. The present study is an attempt to determine what influence the extracellular matrix has on the initial outgrowth of the limb bud. Stage -12 to -18 chick embryo lateral plates were examined in relation to proline and sulfate incorporation into collagen and proteoglycan. The flank and limbs incorporated the same amount of labeled proline and sulfate before stage 16. At stage 16 the flank began to incorporate more of both isotopes until at stage 18 there was twice as much incorporation into the flank as into the limbs. The flank and limbs contained the same type of collagen during the period examined. The limbs contained both large and small proteoglycans but the flank contained only small proteoglycans. These data suggest that the extracellular matrix in the flank and limb regions may play a role in limb outgrowth and that the limb buds at these stages may be more inclined toward cartilage development.

Abstract 311: Osteogenic Transcription Factor Runx2 Represses Connective Tissue Growth Factor Gene Expression And TGFβ Signaling In Vascular Smooth Muscle Cells

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Toru Tanaka ◽  
Takehisa Shimizu ◽  
Norimichi Koitabashi ◽  
Hiroki Matsui ◽  
Hiroshi Doi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Transcription Factor ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Connective Tissue ◽  
Vascular Smooth Muscle Cells ◽  
Tissue Growth ◽  
Matrix Synthesis ◽  
Tgfβ Signaling

[Objective] Runx2, a key transcription factor in osteoblast differentiation, is expressed in calcified atherosclerotic plaques. We have recently shown that Runx2 represses vascular smooth muscle cells (VSMCs) differentiation and promotes their osteogenic differentiation. Connective tissue growth factor (CTGF) has been implicated in the progression to vulnerable plaque by inducing mononuclear cell chemotaxis and VSMCs apoptosis despite of its potent stimulatory effect on connective tissue cell the proliferation and extracellular matrix synthesis. To assess the role of Runx2 in the process of plaque development, we investigated the molecular mechanism of the CTGF gene expression by Runx2 in VSMCs. [Methods and Results] RT-PCR analyses showed that adenovirally overexpressed Runx2 significantly repressed the basal expression of the CTGF gene in human aortic SMCs (HASMCs). Consistent with this, knockdown of the Runx2 expression in HASMCs by small interfering RNA (siRNA) increased CTGF mRNA levels. Luciferase assays showed that Runx2 reduced the transcriptional activity of the CTGF promoter. Transfection of a series of 5′-deletion constructs revealed that Runx2 inhibited CTGF expression through the sequence element located at 5′ untranslated region of CTGF mRNA. We next examined the effects of Runx2 on the TGFβ-induced CTGF expression. Runx2 overexpression significantly repressed CTGF expression in HASMCs stimulated with TGFβ, and knockdown of Runx2 by siRNA enhanced the induction of CTGF expression in response to TGFβ. Runx2 repressed TGFβ-induced CTGF promoter activity through the sequence including Smad binding element (SBE). Overexpression of Runx2 significantly reduced TGFβ- and Smad3-mediated luciferase activity of Smad-dependent promoter which contains four copies of SBE. Biotinylated DNA pulldown assay using SBE of CTGF promoter showed that Runx2 formed a complex with Smad3 and Smad4. [Conclusion] Runx2 repressed basal and TGFβ-induced CTGF gene expression in VSMCs. Thus, in addition to the potential for inducing vascular calcification, Runx2 may affect plaque stability by modulating extracellular matrix synthesis through inhibiting CTGF gene expression and TGFβ signaling.

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