Circadian Dynamics of Cell Composition of the Thymus and Lymph Nodes in Mice Normally, under Conditions of Permanent Illumination, and after Melatonin Injection

Intestinal helminth parasites can alter immune responses to vaccines, other infections, allergens and autoantigens, indicating effects on host immune responses in distal barrier tissues. We herein show that C57BL/6 mice infected with the strictly intestinal nematode Heligmosomoides polygyrus have impaired capacity to initiate skin immune responses and develop skin-resident memory cells to mycobacterial antigens, both during infection and months after deworming therapy. Surprisingly, and in contrast to a previously noted loss of T cells in peripheral lymph nodes, the skin of worm-infected mice harboured higher numbers of CD4+ T cells compared to skin of uninfected controls. H. polygyrus-specific TH2 cells accumulated during infection and remained after worm expulsion. Accumulation of TH2 cells in the skin was associated with increased expression of the skin-homing chemokine receptors CCR4 and CCR10 on CD4+ T cells in blood and mesenteric lymph nodes draining intestinal tissues, indicating gut-to-skin trafficking of cells. In conclusion, we show that infection by a strictly intestinal helminth has long-term effects on immune cell composition and local immune responses to unrelated antigens in the skin, revealing a novel mechanism for T cell colonization and worm-mediated immunosuppression in this organ.

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Cell composition of lymph nodes and DNA content in their various lymphocyte populations in mice with tumors induced by Moloney mouse sarcoma virus

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00838198 ◽

1979 ◽

Vol 88 (4) ◽

pp. 1173-1175

Author(s):

K. A. Gudim-Levkovich ◽

V. M. Yudin ◽

L. P. Lysyuk ◽

S. A. Kovbasyuk ◽

Yu. A. Umanskii

Keyword(s):

Lymph Nodes ◽

Dna Content ◽

Cell Composition ◽

Mouse Sarcoma ◽

Sarcoma Virus ◽

Lymphocyte Populations ◽

Mouse Sarcoma Virus

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Changes in the Structure and Cell Composition of Human Carinal Lymph Nodes during Aging

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-018-4246-z ◽

2018 ◽

Vol 165 (5) ◽

pp. 702-706 ◽

Cited By ~ 1

Author(s):

L. M. Erofeeva ◽

M. V. Mnikhovich

Keyword(s):

Lymph Nodes ◽

Cell Composition

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Scanning electron microscopy of freeze-fractured prescapular lymph node of caprine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111331 ◽

1984 ◽

Vol 42 ◽

pp. 244-245

Author(s):

O. Faroon ◽

F. Al-Bagdadi ◽

T. G. Snider ◽

C. Titkemeyer

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Lymphatic System ◽

Three Dimensional ◽

Meat Products ◽

The Body ◽

Morphological Data ◽

The World ◽

Cellular Constituent ◽

Scanning Electron

The lymphatic system is very important in the immunological activities of the body. Clinicians confirm the diagnosis of infectious diseases by palpating the involved cutaneous lymph node for changes in size, heat, and consistency. Clinical pathologists diagnose systemic diseases through biopsies of superficial lymph nodes. In many parts of the world the goat is considered as an important source of milk and meat products.The lymphatic system has been studied extensively. These studies lack precise information on the natural morphology of the lymph nodes and their vascular and cellular constituent. This is due to using improper technique for such studies. A few studies used the SEM, conducted by cutting the lymph node with a blade. The morphological data collected by this method are artificial and do not reflect the normal three dimensional surface of the examined area of the lymph node. SEM has been used to study the lymph vessels and lymph nodes of different animals. No information on the cutaneous lymph nodes of the goat has ever been collected using the scanning electron microscope.

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Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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