A Method for Determining Characteristic Parameters of DNA Melting in Nucleic Acid Analyzers

2022 ◽  
Author(s):  
V. E. Kurochkin ◽  
D. A. Belov ◽  
Yu. V. Belov ◽  
A. N. Zubik
Keyword(s):  
Nucleic Acid ◽  
Dna Melting ◽  
Characteristic Parameters

The Metric Structure of Nucleic Acids and the Higher Dimension of Their Constituents

2018 ◽  
Vol 7 (2) ◽  
pp. 1-15 ◽  
Author(s):  
Gennadiy Vladimirovich Zhizhin
Keyword(s):  
Nucleic Acid ◽  
Internal Degree ◽  
Chemical Bonds ◽  
Higher Dimension ◽  
Acid Molecule ◽  
Characteristic Parameters ◽  
Experimental Characteristic ◽  
Nucleic Acid Molecule ◽  
Internal Degree Of Freedom ◽  
Angle Of Rotation

The purpose of the study is to build a metric model of the structure of a nucleic acid molecule, taking into account the higher dimension of its components, the actual lengths of chemical bonds, and the angles between them. Calculations by the constructed model showed the closeness of the known experimental characteristic parameters of the nucleic acid (helix diameter, period length) and the calculated values of these parameters. The internal degree of freedom of the nucleic acid molecule is revealed: the angle of rotation of the phosphoric acid residue relative to the chemical bond connecting it to the five-carbon sugar molecule. It is established that the value of this angle determines the shape of the nucleic acid molecule. It is claimed that the process of transmission of hereditary information and protein synthesis occurs in a space of high dimensionality in ribosomes.

Spherical Nucleic Acid Enhanced FO-SPR DNA Melting for Detection of Mutations in Legionella pneumophila

2013 ◽  
Vol 85 (3) ◽  
pp. 1734-1742 ◽  
Author(s):  
Karel Knez ◽  
Kris P. F. Janssen ◽  
Dragana Spasic ◽  
Priscilla Declerck ◽  
Louise Vanysacker ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Legionella Pneumophila ◽  
Dna Melting ◽  
Spherical Nucleic Acid ◽  
Detection Of Mutations

Siphon Flows in Stratified Coronal Loops

1994 ◽  
Vol 144 ◽  
pp. 185-187
Author(s):  
S. Orlando ◽  
G. Peres ◽  
S. Serio
Keyword(s):  
Heat Flux ◽  
Scaling Laws ◽  
Flow Model ◽  
Supersonic Flows ◽  
Coronal Loops ◽  
Characteristic Parameters

AbstractWe have developed a detailed siphon flow model for coronal loops. We find scaling laws relating the characteristic parameters of the loop, explore systematically the space of solutions and show that supersonic flows are impossible for realistic values of heat flux at the base of the upflowing leg.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

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