A completely in vitro system for obtaining scFv using mRNA display, PCR, direct sequencing, and wheat embryo cell-free translation

2009 ◽  
Vol 31 (7) ◽  
pp. 1103-1110 ◽  
Author(s):  
Tatsuro Shibui ◽  
Teruaki Kobayashi ◽  
Keiichiro Kanatani
Keyword(s):  
Direct Sequencing ◽  
Embryo Cell ◽  
Mrna Display ◽  
Wheat Embryo ◽  
In Vitro System ◽  
Free Translation

In vitro selection of scFv and its production: an application of mRNA display and wheat embryo cell-free and E. coli cell production system

2009 ◽  
Vol 84 (4) ◽  
pp. 725-732 ◽  
Author(s):  
Tatsuro Shibui ◽  
Teruaki Kobayashi ◽  
Keiichiro Kanatani ◽  
Hirohisa Koga ◽  
Satoru Misawa ◽  
...  
Keyword(s):  
Production System ◽  
In Vitro Selection ◽  
Embryo Cell ◽  
Coli Cell ◽  
Mrna Display ◽  
Wheat Embryo ◽  
Cell Production ◽  
E Coli ◽  
Selection Of

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

Isolation of globin mRNA from spleen of anemic rabbits and its translation in wheat embryo cell-free system

1978 ◽  
Vol 43 (4) ◽  
pp. 1184-1189
Author(s):  
Ota Fuchs ◽  
Jitka Borová ◽  
Přemysl Poňka ◽  
Jan Neuwirt
Keyword(s):  
Embryo Cell ◽  
Globin Mrna ◽  
Free System ◽  
Wheat Embryo ◽  
Cell Free System

scholarly journals Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlota Oleaga ◽  
Catia Bernabini ◽  
Alec S.T. Smith ◽  
Balaji Srinivasan ◽  
Max Jackson ◽  
...  
Keyword(s):  
In Vitro System ◽  
Organ Toxicity
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