Effects of 5-azacytidine-induced DNA demethylation on polyketide synthase gene expression in larvae of sea urchin Strongylocentrotus intermedius

2016 ◽  
Vol 38 (12) ◽  
pp. 2035-2041 ◽  
Author(s):  
A. P. Tyunin ◽  
N. V. Ageenko ◽  
K. V. Kiselev
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Polyketide Synthase ◽  
Dna Demethylation ◽  
Strongylocentrotus Intermedius ◽  
Polyketide Synthase Gene

scholarly journals Expression of Pigment Cell-Specific Genes in the Ontogenesis of the Sea UrchinStrongylocentrotus intermedius

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Natalya V. Ageenko ◽  
Konstantin V. Kiselev ◽  
Nelly A. Odintsova
Keyword(s):  
Gene Expression ◽  
Cell Differentiation ◽  
Sea Urchin ◽  
Polyketide Synthase ◽  
Shikimic Acid ◽  
Pigment Cell ◽  
Pigment Cells ◽  
Strongylocentrotus Intermedius ◽  
Molecular Signaling ◽  
Gastrula Stage

One of the polyketide compounds, the naphthoquinone pigment echinochrome, is synthesized in sea urchin pigment cells. We analyzed polyketide synthase (pks) and sulfotransferase (sult) gene expression in embryos and larvae of the sea urchinStrongylocentrotus intermediusfrom various stages of development and in specific tissues of the adults. We observed the highest level of expression of thepksandsultgenes at the gastrula stage. In unfertilized eggs, only trace amounts of thepksandsulttranscripts were detected, whereas no transcripts of these genes were observed in spermatozoids. The addition of shikimic acid, a precursor of naphthoquinone pigments, to zygotes and embryos increased the expression of thepksandsultgenes. Our findings, including the development of specific conditions to promote pigment cell differentiation of embryonic sea urchin cells in culture, represent a definitive study on the molecular signaling pathways that are involved in the biosynthesis of pigments during sea urchin development.

scholarly journals Use of the kojA promoter, involved in kojic acid biosynthesis, for polyketide production in Aspergillus oryzae: implications for long-term production

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Koichi Tamano ◽  
Mahoko Kuninaga ◽  
Naoshi Kojima ◽  
Myco Umemura ◽  
Masayuki Machida ◽  
...  
Keyword(s):  
Gene Expression ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Polyketide Synthase ◽  
Kojic Acid ◽  
Continuous Production ◽  
Gene Promoter ◽  
Primary Metabolism ◽  
Polyketide Synthase Gene

Abstract Background Aspergillus oryzae, a useful industrial filamentous fungus, produces limited varieties of secondary metabolites, such as kojic acid. Thus, for the production of valuable secondary metabolites by genetic engineering, the species is considered a clean host, enabling easy purification from cultured cells. A. oryzae has been evaluated for secondary metabolite production utilizing strong constitutive promoters of genes responsible for primary metabolism. However, secondary metabolites are typically produced by residual nutrition after microbial cells grow to the stationary phase and primary metabolism slows. We focused on a promoter of the secondary metabolism gene kojA, a component of the kojic acid biosynthetic gene cluster, for the production of other secondary metabolites by A. oryzae. Results A kojA disruptant that does not produce kojic acid was utilized as a host strain for production. Using this host strain, a mutant that expressed a polyketide synthase gene involved in polyketide secondary metabolite production under the kojA gene promoter was constructed. Then, polyketide production and polyketide synthase gene expression were observed every 24 h in liquid culture. From days 0 to 10 of culture, the polyketide was continuously produced, and the synthase gene expression was maintained. Therefore, the kojA promoter was activated, and it enabled the continuous production of polyketide for 10 days. Conclusions The combined use of the kojA gene promoter and a kojA disruptant proved useful for the continuous production of a polyketide secondary metabolite in A. oryzae. These findings suggest that this combination can be applied to other secondary metabolites for long-term production.

Approaches to stabilization of inter-domain recombination in polyketide synthase gene expression plasmids

2003 ◽  
Vol 30 (3) ◽  
pp. 161-167 ◽  
Author(s):  
Z. Hu ◽  
R. P. Desai ◽  
Y. Volchegursky ◽  
T. Leaf ◽  
E. Woo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polyketide Synthase ◽  
Polyketide Synthase Gene ◽  
Expression Plasmids

Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus

2013 ◽  
Vol 6 (3) ◽  
pp. 291-297 ◽  
Author(s):  
M.L. Ponsone ◽  
Y.G Kuhn ◽  
M Schmidt-Heydt ◽  
R. Geisen ◽  
S.N. Chulze
Keyword(s):  
Gene Expression ◽  
Biosynthetic Pathway ◽  
Transcriptional Activity ◽  
Polyketide Synthase ◽  
Biocontrol Agents ◽  
Inhibitory Mechanism ◽  
Penicillium Species ◽  
Adaptation Mechanism ◽  
Polyketide Synthase Gene ◽  
Mycotoxin Biosynthesis

In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.

DNA Demethylation Regulates Anabolic and Catabolic Gene Expression in Intervertebral Disc Cells

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376587-s-0034-1376587
Author(s):  
N. Chutkan ◽  
R. Sangani ◽  
H. Zhou ◽  
S. Fulzele
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Dna Demethylation ◽  
Catabolic Gene ◽  
Disc Cells
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