Immobilized Enzymes-Based Biosensing Cues for Strengthening Biocatalysis and Biorecognition

A model of biosensor containing three immobilized enzymes utilizing consecutive substrate conversion in the chain was developed. The modeling was performed at an internal diffusion limitation and a steadystate condition. The calculations showed that significant response of biosensors was produced if diffusion modules were larger than 1 for all enzyme reactions. Due to diffusion limitation the apparent stability of biosensor response increased many times in comparison to stability of the most labile enzyme of the chain.

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Enzyme Immobilization on Nanomaterials for Biosensor and Biocatalyst in Food and Biomedical Industry

Current Pharmaceutical Design ◽

10.2174/1381612825666190712181403 ◽

2019 ◽

Vol 25 (24) ◽

pp. 2661-2676 ◽

Cited By ~ 1

Author(s):

Sundaresan Bhavaniramya ◽

Ramar Vanajothi ◽

Selvaraju Vishnupriya ◽

Kumpati Premkumar ◽

Mohammad S. Al-Aboody ◽

...

Keyword(s):

Enzyme Activity ◽

Enzyme Immobilization ◽

Food Industry ◽

Environmental Changes ◽

Immobilized Enzymes ◽

Food Industries ◽

Physiological Processes ◽

Different Types ◽

Simple Processing ◽

Biomedical Industry

Enzymes exhibit a great catalytic activity for several physiological processes. Utilization of immobilized enzymes has a great potential in several food industries due to their excellent functional properties, simple processing and cost effectiveness during the past decades. Though they have several applications, they still exhibit some challenges. To overcome the challenges, nanoparticles with their unique physicochemical properties act as very attractive carriers for enzyme immobilization. The enzyme immobilization method is not only widely used in the food industry but is also a component methodology in the pharmaceutical industry. Compared to the free enzymes, immobilized forms are more robust and resistant to environmental changes. In this method, the mobility of enzymes is artificially restricted to changing their structure and properties. Due to their sensitive nature, the classical immobilization methods are still limited as a result of the reduction of enzyme activity. In order to improve the enzyme activity and their properties, nanomaterials are used as a carrier for enzyme immobilization. Recently, much attention has been directed towards the research on the potentiality of the immobilized enzymes in the food industry. Hence, the present review emphasizes the different types of immobilization methods that is presently used in the food industry and other applications. Various types of nanomaterials such as nanofibers, nanoflowers and magnetic nanoparticles are significantly used as a support material in the immobilization methods. However, several numbers of immobilized enzymes are used in the food industries to improve the processing methods which not only reduce the production cost but also the effluents from the industry.

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Functionalized Materials as a Versatile Platform for Enzyme Immobilization in Wastewater Treatment

Current Pollution Reports ◽

10.1007/s40726-021-00193-5 ◽

2021 ◽

Author(s):

Agnieszka Kołodziejczak-Radzimska ◽

Long D. Nghiem ◽

Teofil Jesionowski

Keyword(s):

Wastewater Treatment ◽

Enzyme Immobilization ◽

Organic Dyes ◽

Scientific Progress ◽

Immobilized Enzymes ◽

Chemical Oxidation ◽

Biologically Active ◽

Water Pollutants ◽

Untreated Wastewater ◽

Functionalized Materials

Abstract Purpose of Review Untreated wastewater discharge can significantly and negatively impact the state of the environment. Rapid industrialization and economic development have directly contributed to land and water pollution resulting from the application of many chemicals such as organic dyes, pharmaceuticals, and industrial reagents. The removal of these chemicals before effluent discharge is crucial for environmental protection. This review aims to explore the importance of functionalized materials in the preparation of biocatalytic systems and consider their application in eliminating water pollutants. Recent Findings Wastewater treatment methods can be classified into three groups: (i) chemical (e.g., chemical oxidation and ozonation), (ii) physical (e.g., membrane separation and ion exchange), and (iii) biological processes. Biological treatment is the most widely used method due to its cost-effectiveness and eco-friendliness. In particular, the use of immobilized enzymes has recently become more attractive as a result of scientific progress in advanced material synthesis. The selection of an appropriate support plays an important role in the preparation of such biologically active systems. Recent studies have demonstrated the use of various materials for enzyme immobilization in the purification of water. Summary This review identifies and discusses different biocatalytic systems used in the enzymatic degradation of various water pollutants. Materials functionalized by specific groups can serve as good support matrices for enzyme immobilization, providing chemical and thermal stability to support catalytic reactions. Enzymatic biocatalysis converts the pollutants into simpler products, which are usually less toxic than their parents. Due to immobilization, the enzyme can be used over multiple cycles to reduce the cost of wastewater treatment. Future studies in this field should focus on developing new platforms for enzyme immobilization in order to improve degradation efficiency.

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Enhanced Wastewater Treatment by Immobilized Enzymes

Current Pollution Reports ◽

10.1007/s40726-021-00183-7 ◽

2021 ◽

Author(s):

Jakub Zdarta ◽

Katarzyna Jankowska ◽

Karolina Bachosz ◽

Oliwia Degórska ◽

Karolina Kaźmierczak ◽

...

Keyword(s):

Wastewater Treatment ◽

Process Optimization ◽

Organic Pollutants ◽

Large Scale ◽

Removal Rate ◽

Immobilized Enzymes ◽

Future Research ◽

Process Conditions ◽

Removal Rates ◽

Catalytic Cycles

Abstract Purpose of Review In the presented review, we have summarized recent achievements on the use of immobilized oxidoreductases for biodegradation of hazardous organic pollutants including mainly dyes, pharmaceuticals, phenols, and bisphenols. In order to facilitate process optimization and achievement of high removal rates, effect of various process conditions on biodegradation has been highlighted and discussed. Recent Findings Current reports clearly show that immobilized oxidoreductases are capable of efficient conversion of organic pollutants, usually reaching over 90% of removal rate. Further, immobilized enzymes showed great recyclability potential, allowing their reuse in numerous of catalytic cycles. Summary Collected data clearly indicates immobilized oxidoreductases as an efficient biocatalytic tools for removal of hazardous phenolic compounds, making them a promising option for future water purification. Data shows, however, that both immobilization and biodegradation conditions affect conversion efficiency; therefore, process optimization is required to achieve high removal rates. Nevertheless, we have demonstrated future trends and highlighted several issues that have to be solved in the near-future research, to facilitate large-scale application of the immobilized oxidoreductases in wastewater treatment.

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