PTEN Gene Induces Cell Invasion and Migration via Regulating AKT/GSK-3β/β-Catenin Signaling Pathway in Human Gastric Cancer

2017 ◽  
Vol 62 (12) ◽  
pp. 3415-3425 ◽  
Author(s):  
Jingjing Ma ◽  
Xufeng Guo ◽  
Jixiang Zhang ◽  
Dandan Wu ◽  
Xue Hu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Signaling Pathway ◽  
Cell Invasion ◽  
Pten Gene ◽  
Human Gastric Cancer ◽  
Invasion And Migration ◽  
And Migration ◽  
Gsk 3Β

Celastrus Orbiculatus Extracts Inhibit the Metastasis through Attenuating PI3K/Akt/mTOR Signaling Pathway in Human Gastric Cancer

2019 ◽  
Vol 19 (14) ◽  
pp. 1754-1761 ◽  
Author(s):  
Yayun Qian ◽  
Yan Yan ◽  
Hongmei Lu ◽  
Tingting Zhou ◽  
Mengying Lv ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Mtor Signaling ◽  
Human Gastric Cancer ◽  
Celastrus Orbiculatus ◽  
Mtor Signaling Pathway ◽  
Gastric Cancer Cells ◽  
Invasion And Migration ◽  
And Migration

Background: Rapamycin receptor inhibitors have been applied in the clinic and achieved satisfactory therapeutic effect recently. The mechanisms did not clearly show how the Celastrus orbiculatus Extracts (COE) inhibited the expression of the mammalian Target of Rapamycin (mTOR) in human gastric cancer cells. The aim of this study was to investigate whether the COE inhibited the metastasis through the mTOR signaling pathway in human gastric cancer MGC-803 cells. Methods: The abnormal expression level of mTOR protein was detected by immunohistochemistry in human gastric cancer tissue. The MGC-803/mTOR- cells were constructed by knockdown of mTOR using lentivirus infection technique. The human gastric cancer MGC-803/mTOR- cells were treated with different concentrations (20, 40, 80 μg/ml) of COE for 24 hours. The ability of cell metastasis was analyzed by the cell invasion and migration assay. The expression levels of PI3K/Akt/mTOR signaling pathway were detected by Western Blotting. Results: COE inhibited the proliferation, invasion and migration of MGC-803/mTOR- cells in a concentrationdependent manner. The expression of E-cadherin protein increased, and the expression of N-cadherin and Vimentin decreased simultaneously in the MGC-803/mTOR- cells. 4EBP1, p-4EBP1, P70S6k, p-P70S6k, mTOR, p-mTOR, PI3K and Akt proteins in MGC-803/mTOR- cells were reduced in a dose-dependent manner. Conclusion: COE could not only inhibit cell growth, invasion and migration, but also inhibit the epithelialmesenchymal transition of gastric cancer cells. The molecular mechanism of COE inhibited the metastasis which may be related to the PI3K/Akt/mTOR signal pathway. This study provides ideas for the development of new anti-gastric cancer drugs.

RSPO2 enhances cell invasion and migration via the WNT/β‐catenin pathway in human gastric cancer

2018 ◽  
Vol 120 (4) ◽  
pp. 5813-5824 ◽  
Author(s):  
Haibo Zhang ◽  
Xiaoyan Han ◽  
Bo Wei ◽  
Jiafeng Fang ◽  
Xuerui Hou ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Invasion ◽  
Human Gastric Cancer ◽  
Invasion And Migration ◽  
And Migration

Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway

2020 ◽  
Vol 216 (7) ◽  
pp. 153001
Author(s):  
Xiumin Qi ◽  
Liang Sun ◽  
Jiayi Wan ◽  
Rongrong Xu ◽  
Songbing He ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Gastric Cancer Cells ◽  
Invasion And Migration ◽  
And Migration ◽  
Gsk 3Β
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