Characterization and differentiation of soft rot causing Pectobacterium carotovorum of Indian origin

2012 ◽  
Vol 136 (1) ◽  
pp. 87-102 ◽  
Author(s):  
Vimal B. Maisuria ◽  
Anuradha S. Nerurkar
2021 ◽  
Vol 31 (1) ◽  
Author(s):  
M’hamed BENADA ◽  
Boualem BOUMAAZA ◽  
Sofiane BOUDALIA ◽  
Omar KHALADI

Abstract Background The development of ecofriendly tools against plant diseases is an important issue in crop protection. Screening and selection process of bacterial strains antagonists of 2 pathogenic bacterial species that limit very important crops, Erwinia amylovora, the causal agent of the fire blight disease, and Pectobacterium carotovorum, the causal agent of bacterial potato soft rot, were reported. Bacterial colonies were isolated from different ecological niches, where both pathogens were found: rhizosphere of potato tubers and fruits and leaves of pear trees from the northwest region of Algeria. Direct and indirect confrontation tests against strains of E. amylovora and P. carotovorum were performed. Results Results showed a significant antagonistic activity against both phytopathogenic species, using direct confrontation method and supernatants of cultures (p<0.005). In vitro assays showed growth inhibitions of both phytopathogenic species. Furthermore, results revealed that the strains of S. plymuthica had a better inhibitory effect than the strains of P. fluorescens against both pathogens. In vivo results on immature pear fruits showed a significant decrease in the progression of the fire blight symptoms, with a variation in the infection index from one antagonistic strain to another between 31.3 and 50%, and slice of potato showed total inhibition of the pathogen (P. carotovorum) by the antagonistic strains of Serratia plymuthica (p<0.005). Conclusion This study highlighted that the effective bacteria did not show any infection signs towards plant tissue, and considered as a potential strategy to limit the fire blight and soft rot diseases.


Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2667-2667 ◽  
Author(s):  
N. Zlatković ◽  
A. Prokić ◽  
K. Gašić ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
...  

2011 ◽  
Vol 62 (4) ◽  
pp. 1411-1417 ◽  
Author(s):  
Meriam Terta ◽  
Souad Azelmat ◽  
Rajaa Ait M’hand ◽  
El Hassan Achbani ◽  
Mustapha Barakate ◽  
...  

2017 ◽  
Vol 107 (11) ◽  
pp. 1322-1330 ◽  
Author(s):  
Huan Jiang ◽  
Mengyi Jiang ◽  
Liuke Yang ◽  
Peiyan Yao ◽  
Lin Ma ◽  
...  

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.


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