Disrupting the quorum sensing mediated virulence in soft rot causing Pectobacterium carotovorum by marine sponge associated Bacillus sp. OA10

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Aparna Anil Singh ◽  
Anil Kumar Singh ◽  
Anuradha Nerurkar
Keyword(s):  
Quorum Sensing ◽  
Marine Sponge ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Bacillus Sp

Detection and characterization of bacteria from the potato rhizosphere degradingN-acyl-homoserine lactone

2006 ◽  
Vol 52 (10) ◽  
pp. 1006-1015 ◽  
Author(s):  
S Jafra ◽  
J Przysowa ◽  
R Czajkowski ◽  
A Michta ◽  
P Garbeva ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Acid Methyl Ester ◽  
Soft Rot ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Pectobacterium Carotovorum ◽  
In Planta ◽  
Lower Efficiency ◽  
Carotovorum Subsp ◽  
First Time

Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we screened bacteria isolated from the potato rhizosphere for the ability to degrade AHLs produced by P. carotovorum subsp. carotovorum. Six isolates able to degrade AHLs were selected for further studies. According to 16S rDNA sequence analysis and fatty acid methyl ester profiling, the isolates belonged to the genera Ochrobactrum, Rhodococcus, Pseudomonas, Bacillus, and Delftia. For the genera Ochrobactrum and Delftia, for the first time AHL-degrading isolates were found. Data presented in this study revealed for the first time that Ochrobactrum sp. strain A44 showed the capacity to inactivate various synthetic AHL molecules; the substituted AHLs were inactivated with a lower efficiency than the unsubstituted AHLs. Compared with the other isolates, A44 was very effective in the degradation of AHLs produced by P. carotovorum subsp. carotovorum. It was verified by polymerase chain reaction, DNA–DNA hybridization, and a lactone ring reconstruction assay that Ochrobactrum sp. strain A44 did not possess AHL lactonase activity. AHL degradation in Ochrobactrum sp. strain A44 occurred intracellularly; it was not found in the culture supernatant. AHL-degrading activity of A44 was thermo sensitive. Experiments in planta revealed that Ochrobactrum sp. strain A44 significantly inhibited the maceration of potato tuber tissue. Since A44 did not produce antibiotics, the attenuation of the decay might be due to the quenching of quorum- sensing-regulated production of pectinolytic enzymes. The strain can potentially serve to control P. carotovorum subsp. carotovorum in potato.Key words: AHL degradation, Ochrobactrum sp., Pectobacterium carotovorum.

scholarly journals Isolation and efficacy of two bacterial strains antagonists of Erwinia amylovora and Pectobacterium carotovorum

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
M’hamed BENADA ◽  
Boualem BOUMAAZA ◽  
Sofiane BOUDALIA ◽  
Omar KHALADI
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Bacterial Species ◽  
Crop Protection ◽  
Soft Rot ◽  
Causal Agent ◽  
Plant Diseases ◽  
Ecological Niches ◽  
Pectobacterium Carotovorum ◽  
Bacterial Strains

Abstract Background The development of ecofriendly tools against plant diseases is an important issue in crop protection. Screening and selection process of bacterial strains antagonists of 2 pathogenic bacterial species that limit very important crops, Erwinia amylovora, the causal agent of the fire blight disease, and Pectobacterium carotovorum, the causal agent of bacterial potato soft rot, were reported. Bacterial colonies were isolated from different ecological niches, where both pathogens were found: rhizosphere of potato tubers and fruits and leaves of pear trees from the northwest region of Algeria. Direct and indirect confrontation tests against strains of E. amylovora and P. carotovorum were performed. Results Results showed a significant antagonistic activity against both phytopathogenic species, using direct confrontation method and supernatants of cultures (p<0.005). In vitro assays showed growth inhibitions of both phytopathogenic species. Furthermore, results revealed that the strains of S. plymuthica had a better inhibitory effect than the strains of P. fluorescens against both pathogens. In vivo results on immature pear fruits showed a significant decrease in the progression of the fire blight symptoms, with a variation in the infection index from one antagonistic strain to another between 31.3 and 50%, and slice of potato showed total inhibition of the pathogen (P. carotovorum) by the antagonistic strains of Serratia plymuthica (p<0.005). Conclusion This study highlighted that the effective bacteria did not show any infection signs towards plant tissue, and considered as a potential strategy to limit the fire blight and soft rot diseases.

scholarly journals First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2667-2667 ◽  
Author(s):  
N. Zlatković ◽  
A. Prokić ◽  
K. Gašić ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
...  
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
First Report ◽  
Carotovorum Subsp

Characterization and differentiation of soft rot causing Pectobacterium carotovorum of Indian origin

2012 ◽  
Vol 136 (1) ◽  
pp. 87-102 ◽  
Author(s):  
Vimal B. Maisuria ◽  
Anuradha S. Nerurkar
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Indian Origin

Molecular typing of Pectobacterium carotovorum isolated from potato tuber soft rot in Morocco

2011 ◽  
Vol 62 (4) ◽  
pp. 1411-1417 ◽  
Author(s):  
Meriam Terta ◽  
Souad Azelmat ◽  
Rajaa Ait M’hand ◽  
El Hassan Achbani ◽  
Mustapha Barakate ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Molecular Typing ◽  
Soft Rot ◽  
Pectobacterium Carotovorum

Inhibition of biofilm formation and quorum sensing mediated virulence in Pseudomonas aeruginosa by marine sponge symbiont Brevibacterium casei strain Alu 1

2021 ◽  
Vol 150 ◽  
pp. 104693
Author(s):  
Nagasundaram Rashiya ◽  
Nagarajan Padmini ◽  
Antony Alex Kennedy Ajilda ◽  
Pandiyan Prabakaran ◽  
Ravindran Durgadevi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Marine Sponge ◽  
Biofilm Formation

scholarly journals Erratum for George et al., “Interactions of Salmonella enterica Serovar Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot”

2018 ◽  
Vol 84 (12) ◽  
Author(s):  
Andrée S. George ◽  
Clayton E. Cox ◽  
Prerak Desai ◽  
Steffen Porwollik ◽  
Weiping Chu ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Serovar Typhimurium
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