Serratia marcescens associated with bell pepper (Capsicum annuum L.) soft-rot disease under greenhouse conditions

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

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Effects of Culture Conditions on the Antibacterial Activity of Streptomyces Spp. against Erwinia Spp. Causing Soft Rot Disease on Asparagus Officinalis

Vietnam Journal of Agricultural Sciences ◽

10.31817/vjas.2021.4.2.08 ◽

2021 ◽

Vol 4 (2) ◽

pp. 1077-1086

Author(s):

Nguyen Thanh Trung ◽

Nguyen Thi Van Anh ◽

Tran Thi Dao ◽

Nguyen Thanh Huyen ◽

Pham Le Anh Minh ◽

...

Keyword(s):

Antibacterial Activity ◽

Ornamental Plants ◽

Soft Rot ◽

Antibacterial Activities ◽

Culture Conditions ◽

Asparagus Officinalis ◽

Effective Control ◽

Soft Rot Disease ◽

Streptomyces Spp ◽

Rot Disease

Erwinia is a genus of Enterobacteriacea containing mostly pathogens, which cause soft rot disease in many ornamental plants and crops, including Asparagus officinalis. Chemical treatments to control Erwinia have lost their attractiveness because of the development of resistant strains and the negative impacts on the environment and human health. Therefore, the study of biological controls of soft rot disease has gained great importance. There are several types of microorganisms that show activity against Erwinia spp. such as Pseudomonas fluorescence, Bacillus subtilis, and Streptomyces spp. Among them, Streptomyces spp. are found to be the most effective control agents. In this study, 64 isolates of Streptomyces were screened for their antibacterial activity against Erwinia spp. The results indicated that 18 isolates showed an antagonistic reaction against Erwinia spp. Among them, isolate D5.1 showed the highest inhibition activity. In addition, the morphological and antibacterial activities of isolate D5.1 grown in different conditions were also characterized.

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TEORES DE MICRONUTRIENTES NO DESENVOLVIMENTO DA PLANTA DE PIMENTÃO SOB FERTIRRIGAÇÃO

Irriga ◽

10.15809/irriga.2003v8n2p120-131 ◽

2003 ◽

Vol 8 (2) ◽

pp. 120-131 ◽

Cited By ~ 1

Author(s):

Francisco Fernando Noronha Marcussi ◽

Roberto Lyra Villas Bôas

Keyword(s):

Capsicum Annuum ◽

Growth Period ◽

Bell Pepper ◽

Soil Science ◽

Pepper Plant ◽

Cu Content ◽

Capsicum Annuum L ◽

Entire Plant ◽

Micronutrient Concentration ◽

E Mail

TEORES DE MICRONUTRIENTES NO DESENVOLVIMENTO DA PLANTA DE PIMENTÃO SOB FERTIRRIGAÇÃO Francisco Fernando Noronha MarcussiDepartamento de Engenharia Rural, Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected] Lyra Villas BôasDepartament de Recursos Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected] 1 RESUMO Neste estudo, objetivou-se determinar os teores de micronutrientes (Fe, Zn, Mn, B e Cu) nos diferentes órgãos do híbrido de pimentão Elisa, sob fertirrigação em ambiente protegido, em oito épocas de coleta de planta. O experimento foi desenvolvido nas dependências do Departamento de Recursos Naturais - Ciência do Solo, FCA, Botucatu/SP. Constou o experimento de 8 tratamentos (épocas de coleta de planta – 0, 20, 40, 60, 80, 100, 120, e 140 dias após o transplante das mudas) e 4 repetições, sendo cada repetição formada por 4 plantas. Em cada etapa obteve-se os teores dos micronutrientes no fruto, folha, caule e raiz e na planta como um todo. A fertilização no ciclo estudado, 140 dias, foi feita via fertirrigação localizada. Os resultados mostram que os teores de micronutrientes encontrados na planta de pimentão seguem a seqüência: Fe>Zn>Mn>B>Cu. Nos resultados são apresentadas as tabelas com os teores de Fe, Zn, Mn, B e Cu (Tukey a 5%) em cada órgão da planta e da planta como um todo, nas diferentes épocas de avaliação e, os gráficos mostrando o comportamento dos teores de Zn, Mn, B e Cu. UNITERMOS: Fertirrigação, concentrações de micronutrientes, Pimentão, Capsicum annuum L. e nutrição mineral. MACUSSI, F.F.N.; VILLAS BÔAS, R.L MICRONUTRIENT CONCENTRATION IN THE BELL PEPPER PLANT DEVELOPMENT UNDER FERTIRRIGATION 2 ABSTRACT This study aimed to determine the micronutrient contents (Fe, Zn, Mn, B and Cu) in different organs of Elisa bell pepper hybrid under fertirrigation in protected environment in eight harvesting seasons. The experiment was carried out in the Natural Resource Department – Soil Science, FCA, Botucatu. SP, and consisted of 8 treatments (harvesting season – 0, 20, 40, 60, 80, 100 and 140 days after seedling transplant), and 4 replications, each one composed of 4 plants. In each growth period the micronutrient content in the fruit, leaf, stem, root and plant as a whole was determined. The fertilization in the study period i.e., 140 days, was performed by local fertirrigation. The results showed that the micronutrient levels found in the bell pepper plant followed the decreasing order: Fe>Zn>Mn> B>Cu. The tables present the Fe, Zn, Mn, B and Cu (Tukey Test at 5%) contents in each plant organ and in the entire plant at different evaluation periods. The charts show the Zn, Mn, B and Cu content variation. KEYWORDS: fertirrigation, micronutrient concentration, bell pepper, Capsicum annuum L. and mineral nutrition.

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