In situ localization of vasotocin receptor gene transcripts in the brain-pituitary-gonadal axis of the catfish Heteropneustes fossilis: a morpho-functional study

2018 ◽  
Vol 45 (3) ◽  
pp. 885-905 ◽  
Author(s):  
Arpana Rawat ◽  
Radha Chaube ◽  
Keerrikkattil P. Joy
Keyword(s):  
Receptor Gene ◽  
Functional Study ◽  
Heteropneustes Fossilis ◽  
Gene Transcripts ◽  
The Brain

Cloning of the rat P2u receptor and its potential role in coronary vasodilation

1996 ◽  
Vol 270 (2) ◽  
pp. C570-C577 ◽  
Author(s):  
S. Godecke ◽  
U. K. Decking ◽  
A. Godecke ◽  
J. Schrader
Keyword(s):  
Blood Vessels ◽  
Rat Heart ◽  
Receptor Gene ◽  
Coding Region ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Coronary Blood Vessels ◽  
The Brain ◽  
Isolated Perfused Rat Hearts

We cloned and sequenced the cDNA as well as the genomic DNA of the P2u receptor gene from the rat. The coding region of the gene is not interrupted by introns. P2u is expressed in a variety of rat organs with pronounced differences of expression intensities. Highest expression was found in liver and testis, while no expression could be detected in the brain. High P2u expression was found in primary microvascular endothelial cells from the rat heart, but not in cardiac myocytes. By in situ analysis, we localized P2u expression in epithelial cells of esophagus and bronchi. Functional analysis revealed that, in isolated perfused rat hearts, the P2u ligands UTP and ATP induce a pronounced vasodilation of coronary blood vessels. In contrast, UMP and uridine, the degradative products of UTP, act as potent vasoconstrictors. Our experiments suggest that, in the rat heart, endothelial P2u receptors are involved in the ATP/UTP-mediated vasodilation of coronary blood vessels.

Neuronal activity and CRF receptor gene transcription in the brains of rats with colitis

2004 ◽  
Vol 287 (4) ◽  
pp. G803-G814 ◽  
Author(s):  
Christophe Porcher ◽  
Valérie Sinniger ◽  
Aurélie Juhem ◽  
Patrick Mouchet ◽  
Bruno Bonaz
Keyword(s):  
Area Postrema ◽  
Receptor Gene ◽  
Circumventricular Organs ◽  
Male Rats ◽  
Frozen Sections ◽  
Trinitrobenzenesulfonic Acid ◽  
In Situ Hybridization Histochemistry ◽  
Neuroanatomical Data ◽  
The Brain

We aimed to characterize neuronal and corticotropin-releasing factor (CRF) pathways at the acute phase of a model of colitis in rats. Male rats received an intracolonic injection of either vehicle (controls) or trinitrobenzenesulfonic acid (TNBS) and were killed 1, 2, 3, 4, 6, 12, or 24 h later. Coronal frozen sections of the brain were cut and mRNAs encoding the rat c- fos, CRF1 receptor, and CRF2α,β receptors were assayed by in situ hybridization histochemistry. Localization of these transcripts within CRF-immunoreactive (CRF-ir) neurons of the paraventricular nucleus (PVN) of the hypothalamus was also determined. Intracolonic TNBS induced c- fos mRNA expression in brain nuclei involved in the autonomic, behavioral, and neuroendocrine response to a stimulus (PVN, amygdala, locus coeruleus, parabrachial nucleus, nucleus of the solitary tract) and in circumventricular organs (lamina terminalis, subfornical organ, area postrema). CRF pathways, particularly in the PVN, were activated in this model as represented by a robust signal of c- fos and CRF1 receptor transcripts in the PVN and numerous CRF-ir neurons expressed c- fos or CRF1 receptor transcripts in the PVN of TNBS-treated animals. No expression of CRF2 receptor transcripts was observed in the PVN, either in basal conditions or after TNBS. These neuroanatomical data argue for an involvement of CRF pathways, through CRF1 receptor, within the PVN in TNBS-induced colitis.

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

Substituted N,N-Dimethyl-3-(phenylthio)- and -4-(phenylthio)benzylamines

1992 ◽  
Vol 57 (1) ◽  
pp. 194-203 ◽  
Author(s):  
Karel Šindelář ◽  
Vojtěch Kmoníček ◽  
Marta Hrubantová ◽  
Zdeněk Polívka
Keyword(s):  
Serotonin Receptors ◽  
Hydrobromic Acid ◽  
Antidepressant Activity ◽  
Benzoic Acids ◽  
Lithium Aluminium Hydride ◽  
Acid Chlorides ◽  
Activity Inhibition ◽  
Lithium Aluminium ◽  
The Brain

(Arylthio)benzoic acids IIa - IIe and VIb - VId were transformed via the acid chlorides to the N,N-dimethylamides which were reduced either with diborane "in situ" or with lithium aluminium hydride to N,N-dimethyl-(arylthio)benzylamines Ia - Ie and Vb - Vd. Leuckart reaction of the aldehydes IX and X with dimethylformamide and formic acid afforded directly the amines Va and Ve. Demethylation of the methoxy compounds Ia and Ve with hydrobromic acid resulted in the phenolic amines If and Vf. The most interesting N,N-dimethyl-4-(phenylthio)benzylamine (Va) hydrochloride showed affinity to cholinergic and 5-HT2 serotonin receptors in the rat brain and some properties considered indicative of antidepressant activity (inhibition of serotonin re-uptake in the brain and potentiation of yohimbine toxicity in mice).

On the role of the selective silencer Freud-1 in the regulation of the brain 5-HT1A receptor gene expression

2010 ◽  
Vol 44 (5) ◽  
pp. 795-800 ◽  
Author(s):  
V. C. Naumenko ◽  
D. V. Osipova ◽  
A. S. Tsybko
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
The Brain ◽  
Receptor Gene Expression

Assignment of the Human Macrophage Mannose Receptor Gene (MRC1) to 10p13 by in Situ Hybridization and PCR-Based Somatic Cell Hybrid Mapping

Genomics ◽  
1994 ◽  
Vol 22 (3) ◽  
pp. 656-658 ◽  
Author(s):  
Quentin Eichbaum ◽  
Phillipe Clerc ◽  
Gall Bruns ◽  
Frank McKeon ◽  
R.Alan B. Ezekowitz
Keyword(s):  
In Situ Hybridization ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Receptor Gene ◽  
Mannose Receptor ◽  
Human Macrophage ◽  
Hybrid Mapping ◽  
Macrophage Mannose Receptor

Assignment of the human ubiquitous receptor gene (UNR) to 19q13.3 using fluorescence in situ hybridization

Genomics ◽  
1995 ◽  
Vol 26 (1) ◽  
pp. 166-168 ◽  
Author(s):  
Michelle M. Le Beau ◽  
Ching Song ◽  
Elizabeth M. Davis ◽  
Richard A. Hiipakka ◽  
John M. Kokontis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Receptor Gene

Ionotropic glutamate-receptor gene expression in hypothalamus: Localization of AMPA, kainate, and NMDA receptor RNA with in situ hybridization

1994 ◽  
Vol 343 (3) ◽  
pp. 428-444 ◽  
Author(s):  
Anthony N. Van Den Pol ◽  
Irm Hermans-Borgmeyer ◽  
Magdalena Hofer ◽  
Prabhat Ghosh ◽  
Stephen Heinemann
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Nmda Receptor ◽  
Glutamate Receptor ◽  
Receptor Gene ◽  
Ionotropic Glutamate Receptor ◽  
Receptor Gene Expression
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