Activin receptor type IIB in rohu (Labeo rohita): molecular characterization, tissue distribution and immunohistochemical localization during different stages of gonadal maturation

Abstract Activin receptor type IIB (ActRIIB) is a transmembrane serine/threonine kinase receptor which plays a pivotal role in regulating reproduction in vertebrates including teleost. Earlier studies have documented its importance in governing gonadal maturation in higher vertebrates. However, reports on regulation of fish reproductive system by ActRIIB gene are still limited. Here, we report identification and characterization of ActRIIB cDNA of Labeo rohita, a commercially important fish species of the Indian subcontinent. The full-length gene encoding rohu ActRIIB was cloned and found to be of 1674 bp in length. Functional similarities were evident form evolutionary analysis across vertebrates. Real-time PCR to measure the expression of ActRIIB transcript in rohu revealed significant mRNA levels in gonads followed by non-reproductive tissues, including brain, pituitary and muscle. With respect to different gonadal maturation stages, predominant expression of ActRIIB mRNA was observed during the pre-spawning phase of both sexes. To further delineate its role in rohu reproduction, a recombinant protein of the extracellular domain of ActRIIB (rECD-ActRIIB) was produced and polyclonal antibody raised against the protein for its immuno-localization studies during different gonadal maturation stages. Strong immunoreactivity was noticed in the pre-vitellogenic oocytes which decreased dramatically in the fully mature oocytes. Similarly, the strong and intense immunoreactivity was found in the spermatids and spermatocytes of the immature testis, and eventually the intensity reduced with the progression of the maturation stage. These results provide the first evidence of presence of ActRIIB in rohu gonadal tissues. Taken together, our observations lay the groundwork for further understanding and investigation on the potential role of ActRIIB in fish reproduction system in the event of gonadal maturation.

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Molecular characterization and computational structure prediction of activin receptor type IIB in aseel and broiler chicken

Research in Veterinary Science ◽

10.1016/j.rvsc.2019.08.025 ◽

2019 ◽

Vol 126 ◽

pp. 139-149

Author(s):

P. Guru Vishnu ◽

T.K. Bhattacharya ◽

Bharat Bhushan ◽

Pushpendra Kumar ◽

R.N. Chatterjee ◽

...

Keyword(s):

Molecular Characterization ◽

Structure Prediction ◽

Broiler Chicken ◽

Receptor Type ◽

Activin Receptor ◽

Computational Structure

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Molecular characterization and tissue distribution of aryl hydrocarbon receptor nuclear translocator isoforms, ARNT1 and ARNT2, and identification of novel splice variants in common cormorant (Phalacrocorax carbo)

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology ◽

10.1016/j.cbpc.2007.01.007 ◽

2007 ◽

Vol 145 (3) ◽

pp. 379-393 ◽

Cited By ~ 11

Author(s):

Jin-Seon Lee ◽

Eun-Young Kim ◽

Hisato Iwata ◽

Shinsuke Tanabe

Keyword(s):

Aryl Hydrocarbon Receptor ◽

Tissue Distribution ◽

Molecular Characterization ◽

Splice Variants ◽

Phalacrocorax Carbo ◽

Aryl Hydrocarbon ◽

Common Cormorant

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Molecular characterization of the gonadal kisspeptin system: Cloning, tissue distribution, gene expression analysis and localization in sablefish ( Anoplopoma fimbria )

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2015.07.015 ◽

2016 ◽

Vol 225 ◽

pp. 212-223 ◽

Cited By ~ 14

Author(s):

Marian R. Fairgrieve ◽

Yasushi Shibata ◽

Elizabeth K. Smith ◽

Edward S. Hayman ◽

J. Adam Luckenbach

Keyword(s):

Gene Expression ◽

Tissue Distribution ◽

Molecular Characterization ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Anoplopoma Fimbria

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A Soluble Activin Receptor Type IIB Prevents the Effects of Androgen Deprivation on Body Composition and Bone Health

Endocrinology ◽

10.1210/en.2010-0134 ◽

2010 ◽

Vol 151 (9) ◽

pp. 4289-4300 ◽

Cited By ~ 46

Author(s):

Alan Koncarevic ◽

Milton Cornwall-Brady ◽

Abigail Pullen ◽

Monique Davies ◽

Dianne Sako ◽

...

Keyword(s):

Body Composition ◽

Muscle Mass ◽

Serum Levels ◽

Androgen Deprivation ◽

Receptor Type ◽

Whole Body ◽

Micro Computed Tomography ◽

Tissue Mass ◽

Activin Receptor

Androgen deprivation, a consequence of hypogonadism, certain cancer treatments, or normal aging in men, leads to loss of muscle mass, increased adiposity, and osteoporosis. In the present study, using a soluble chimeric form of activin receptor type IIB (ActRIIB) we sought to offset the adverse effects of androgen deprivation on muscle, adipose tissue, and bone. Castrated (ORX) or sham-operated (SHAM) mice received either TBS [vehicle-treated (VEH)] or systemic administration of ActRIIB-mFc, a soluble fusion protein comprised of a form of the extracellular domain of ActRIIB fused to a murine IgG2aFc subunit. In vivo body composition imaging demonstrated that ActRIIB-mFc treatment results in increased lean tissue mass of 23% in SHAM mice [19.02 ± 0.42 g (VEH) versus 23.43 ± 0.35 g (ActRIIB-mFc), P < 0.00001] and 26% in ORX mice [15.59 ± 0.26 g (VEH) versus 19.78 ± 0.26 g (ActRIIB-mFc), P < 0.00001]. Treatment also caused a decrease in adiposity of 30% in SHAM mice [5.03 ± 0.48 g (VEH) versus 3.53 ± 0.19 g (ActRIIB-mFc), NS] and 36% in ORX mice [7.12 ± 0.53 g (VEH) versus 4.57 ± 0.28 g (ActRIIB-mFc), P < 0.001]. These changes were also accompanied by altered serum levels of leptin, adiponectin, and insulin, as well as by prevention of steatosis (fatty liver) in ActRIIB-mFc-treated ORX mice. Finally, ActRIIB-mFc prevented loss of bone mass in ORX mice as assessed by whole body dual x-ray absorptiometry and micro-computed tomography of proximal tibias. The data demonstrate that treatment with ActRIIB-mFc restored muscle mass, adiposity, and bone quality to normal levels in a mouse model of androgen deprivation, thereby alleviating multiple adverse consequences of such therapy.

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