Response to copper and cadmium stress in wild-type and copper tolerant strains of the lichen alga Trebouxia erici: metal accumulation, toxicity and non-protein thiols

2007 ◽  
Vol 52 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Martin Bačkor ◽  
Barbara Pawlik-Skowrońska ◽  
Jana Bud’ová ◽  
Tadeusz Skowroński
Keyword(s):  
Metal Accumulation ◽  
Cadmium Stress ◽  
Wild Type ◽  
Protein Thiols ◽  
Copper Tolerant

scholarly journals Use of Fatty Acid Methyl Ester Profiles to Compare Copper-Tolerant and Copper-Sensitive Strains of Pantoea ananatis

2007 ◽  
Vol 97 (10) ◽  
pp. 1298-1304 ◽  
Author(s):  
C. Nischwitz ◽  
R. Gitaitis ◽  
H. Sanders ◽  
D. Langston ◽  
B. Mullinix ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Wild Type ◽  
Pantoea Ananatis ◽  
Sulfate Pentahydrate ◽  
Acid Methyl ◽  
Copper Sulfate Pentahydrate ◽  
Copper Tolerant ◽  
Tolerant Clones

A survey was conducted to evaluate differences in fatty acid methyl ester (FAME) profiles among strains of Pantoea ananatis, causal agent of center rot of onion (Allium cepa), isolated from 15 different onion cultivars in three different sites in Georgia. Differences in FAME composition were determined by plotting principal components (PCs) in two-dimensional plots. Euclidean distance squared (ED2) values indicated a high degree of similarity among strains. Plotting of PCs calculated from P. ananatis strains capable of growing on media amended with copper sulfate pentahydrate (200 μg/ml) indicated that copper-tolerant strains grouped into tight clusters separate from clusters formed by wild-type strains. However, unlike copper-sensitive strains, the copper-tolerant strains tended to cluster by location. A total of 80, 60, and 73% of the strains from Tift1, Tift2, and Tattnall, respectively, exhibited either confluent growth or partial growth on copper-amended medium. However, all strains were sensitive to a mixture of copper sulfate pentahydrate (200 μg/ml) and maneb (40 μg/ml). When copper-tolerant clones were analyzed and compared with their wild-type parents, in all cases the plotting of PCs developed from copper-tolerant clones formed tight clusters separate from clusters formed by the parents. Eigenvalues generated from these tests indicated that two components provided a good summary of the data, accounting for 98, 98, and 96% of the standardized variance for strains Pna 1-15B, Pna 1-12B, and Pna 2-5A, respectively. Furthermore, feature 4 (cis-9-hexadecenoic acid/2-hydroxy-13-methyltetradecanoic acid) and feature 7 (cis-9/trans-12/cis-7-octadecenoic acid) were the highest or second highest absolute values for PC1 in all three strains of the parents versus copper-tolerant clones, and hexadecanoic acid was the highest absolute value for PC2 in all three strains. Along with those fatty acids, dodecanoic acid and feature 3 (3-hydroxytetradecanoic acid/14-methylpentadecenoic acid) also had an impact on the differences observed between copper-sensitive parents and copper-resistant mutants. Finding these changes in bacterial fatty acid composition could lead to the development of a laboratory assay to identify copper-tolerant strains using gas chromatography as well as providing clues to further elucidate the mode of action of copper tolerance.

Metal Accumulation in Estuarine Plants: Investigating the Effect on the Levels of Non-protein Thiols in Roots of Different Salt Marsh Plants

2018 ◽  
pp. 185-205
Author(s):  
A. Cristina S. Rocha ◽  
Simone Cavenati ◽  
M. Teresa S. D. Vasconcelos ◽  
M. Clara P. Basto ◽  
C. Marisa R. Almeida
Keyword(s):  
Salt Marsh ◽  
Metal Accumulation ◽  
Salt Marsh Plants ◽  
Protein Thiols

scholarly journals The Transcriptional Response of Lactobacillus sanfranciscensis DSM 20451Tand ItstcyBMutant Lacking a Functional Cystine Transporter to Diamide Stress

2014 ◽  
Vol 80 (14) ◽  
pp. 4114-4125 ◽  
Author(s):  
Mandy Stetina ◽  
Jürgen Behr ◽  
Rudi F. Vogel
Keyword(s):  
Oxidative Stress ◽  
Antioxidative Enzymes ◽  
Stress Responses ◽  
Cell Damage ◽  
Transcriptional Response ◽  
Wild Type ◽  
Content Type ◽  
Lactobacillus Sanfranciscensis ◽  
Cystine Transporter ◽  
Protein Thiols

ABSTRACTAs a result of its strong adaptation to wheat and rye sourdoughs,Lactobacillus sanfranciscensishas the smallest genome within the genusLactobacillus. The concomitant absence of some important antioxidative enzymes and the inability to synthesize glutathione suggest a role of cystine transport in maintenance of an intracellular thiol balance. Diamide [synonym 1,1′-azobis(N,N-dimethylformamide)] disturbs intracellular and membrane thiol levels in oxidizing protein thiols depending on its initial concentration. In this study, RNA sequencing was used to reveal the transcriptional response ofL. sanfranciscensisDSM 20451T(wild type [WT]) and its ΔtcyBmutant with a nonfunctional cystine transporter after thiol stress caused by diamide. Along with the different expression of genes involved in amino acid starvation, pyrimidine synthesis, and energy production, our results show that thiol stress in the wild type can be compensated through activation of diverse chaperones and proteases whereas the ΔtcyBmutant shifts its metabolism in the direction of survival. Only a small set of genes are significantly differentially expressed between the wild type and the mutant. In the WT, mainly genes which are associated with a heat shock response are upregulated whereas glutamine import and synthesis genes are downregulated. In the ΔtcyBmutant, the wholeoppoperon was more highly expressed, as well as a protein which probably includes enzymes for methionine transport. The two proteins encoded byspxAandnrdH, which are involved in direct or indirect oxidative stress responses, are also upregulated in the mutant. This work emphasizes that even in the absence of definitive antioxidative enzymes, bacteria with a small genome and a high frequency of gene inactivation and elimination use small molecules such as the cysteine/cystine couple to overcome potential cell damage resulting from oxidative stress.

Effects of exogenous calcium and spermidine on cadmium stress moderation and metal accumulation in Boehmeria nivea (L.) Gaudich

2016 ◽  
Vol 23 (9) ◽  
pp. 8699-8708 ◽  
Author(s):  
Xiaomin Gong ◽  
Yunguo Liu ◽  
Danlian Huang ◽  
Guangming Zeng ◽  
Shaobo Liu ◽  
...  
Keyword(s):  
Metal Accumulation ◽  
Cadmium Stress ◽  
Boehmeria Nivea ◽  
Exogenous Calcium

scholarly journals Investigating the ability of Pseudomonas fluorescens UW4 to reduce cadmium stress in Lactuca sativa via an intervention in the ethylene biosynthetic pathway

2016 ◽  
Vol 62 (12) ◽  
pp. 1057-1062 ◽  
Author(s):  
Lucas J. Albano ◽  
Sheila M. Macfie
Keyword(s):  
Pseudomonas Fluorescens ◽  
Lactuca Sativa ◽  
Ethylene Production ◽  
Bacterial Colonization ◽  
Acc Deaminase ◽  
Fold Increase ◽  
Cadmium Stress ◽  
Root Surface ◽  
Wild Type ◽  
Ethylene Concentration

A typical plant response to any biotic or abiotic stress, including cadmium (Cd), involves increased ethylene synthesis, which causes senescence of the affected plant part. Stressed plants can experience reduced ethylene and improved growth if they are inoculated with bacteria that have the enzyme ACC deaminase, which metabolizes the ethylene precursor ACC (1-aminocyclopropane-1-carboxylate). We investigated whether one such bacterium, Pseudomonas fluorescens UW4, reduces the production of ethylene and improves the growth of lettuce (Lactuca sativa) sown in Cd-contaminated potting material (PRO-MIX® BX). Plants were inoculated with the wild-type P. fluorescens UW4 or a mutant strain that cannot produce ACC deaminase. Cadmium-treated plants contained up to 50 times more Cd than did control plants. In noninoculated plants, Cd induced a 5-fold increase in ethylene concentration. The wild-type bacterium prevented Cd-induced reductions in root biomass but there was no relationship between Cd treatment and ethylene production in inoculated plants. In contrast, when the concentration of ethylene was plotted against the extent of bacterial colonization of the roots, increased colonization with wild-type P. fluorescens UW4 was associated with 20% less ethylene production. Ours is the first study to show that the protective effect of this bacterium is proportional to the quantity of bacteria on the root surface.

Metal accumulation behavior of the weed species growing under soil cadmium stress

2019 ◽  
Vol 51 (4) ◽  
Author(s):  
Md. Dulal Sarkar ◽  
Md. Jahedur Rahman ◽  
Jasim Uddin ◽  
Md Quamruzzaman ◽  
Rojobi Nahar Rojoni ◽  
...  
Keyword(s):  
Metal Accumulation ◽  
Cadmium Stress ◽  
Weed Species ◽  
Soil Cadmium ◽  
Accumulation Behavior

scholarly journals Translational asymmetry as a sensitive indicator of cadmium stress in plants: a laboratory test with wild-type and mutant Arabidopsis thaliana

2003 ◽  
Vol 159 (2) ◽  
pp. 471-477 ◽  
Author(s):  
Ariadne Tan-Kristanto ◽  
Ary Hoffmann ◽  
Richard Woods ◽  
Phil Batterham ◽  
Christopher Cobbett ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Laboratory Test ◽  
Cadmium Stress ◽  
Sensitive Indicator ◽  
Wild Type

scholarly journals Protein lysine methylation is involved in modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress

2019 ◽  
Author(s):  
Nelson B.C. Serre ◽  
Manon Sarthou ◽  
Océane Gigarel ◽  
Sylvie Figuet ◽  
Massimiliano Corso ◽  
...  
Keyword(s):  
Cadmium Stress ◽  
Cadmium Accumulation ◽  
Lysine Methylation ◽  
Wild Type ◽  
Sensitive Species ◽  
Lysine Residues ◽  
Methyltransferase Gene ◽  
Lysine Methyltransferase ◽  
Tolerance To Cadmium ◽  
Arabidopsis Species

ABSTRACTThe mechanisms underlying the response and adaptation of plants to excess of trace elements are not fully described. Here, we analyzed the importance of protein lysine methylation for plants to cope with cadmium. We analyzed the effect of cadmium on lysine-methylated proteins and protein lysine methyltransferases (KMTs) in two cadmium-sensitive species, Arabidopsis thaliana and A. lyrata, and in three populations of A. halleri with contrasting cadmium accumulation and tolerance traits. We showed that some proteins are differentially methylated at lysine residues in response to Cd and that a few genes coding KMTs is regulated by cadmium. Also, we showed that nine out of 23 A. thaliana mutants interrupted in KMT genes have a tolerance to cadmium that is significantly different from that of wild-type seedlings. We further characterized two of these mutants, one was knocked-out in the calmodulin lysine methyltransferase gene and displayed increased tolerance to cadmium, the other was interrupted in a KMT gene of unknown function and showed a decreased capacity to cope with cadmium. Together, our results showed that lysine methylation of non-histone proteins is impacted by cadmium and that several methylation events are important for modulating the response of Arabidopsis plants to cadmium stress.

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