Kinetics of low-temperature catalytic combustion of ethylene at wet conditions for postharvest storage applications

The study addresses the reduction of ethylene levels in postharvest storage applications using a Pd-Zn-Sn/TiO2 catalyst, which is capable of reacting trace concentrations of ethylene at temperatures as low as 278 K and at relative humidity as high as 90%. The rate law is derived from data collected using a constant volume batch reactor and a model for a storage room with associated packed bed reactor is developed. The amount of catalyst required to maintain an ethylene concentration of 0.1 ppmv in a room containing 20 tons of fruit having an ethylene metabolism of 0.1 ul/kg hr was calculated as a function of air temperature and water content. While the catalyst is capable of continuously removing ethylene from saturated, refrigerated air, the amount of catalyst required can be reduced significantly by incorporating conventional air conditioning solutions upstream of the catalyst bed. Such combined systems and their functions are discussed

Physiological changes in green and red cherry tomatoes after photocatalytic ethylene degradation using continuous air flux

In this work photocatalytic ethylene degradation (TiO2-UV) was applied in green cherry tomatoes with the aim to control biochemical and physiological changes during ripening. Photocatalytic process was performed at 18 °C ± 2 °C and 85% HR for 10 days using continuous air flux. Ethylene, O2 and CO2 concentration from cherry tomatoes under TiO2-UV and control (c) fruits, were measured by GC-MS for 10 days. After that, the tomatoes were stored for 20 days. During the photocatalysis process, ethylene was completely degraded and control fruits, the ethylene was 28.73 nL g−1. Respiration rate was lower for fruits under TiO2-UV than control. During storage period, cherry tomatoes treated by TiO2-UV, showed lower ethylene concentration, respiration rate, total soluble solid, lycopene, sugar and organic acid content than control showing that the fruits treated with photocatalysis did not reach the full maturity. In addition, all the cherry tomatoes showed different maturity stages. Fungal incidence was higher in control fruits than fruits treated with photocatalysis. This research showed for the first time that photocatalytic technology preserved the physiological quality of cherry tomatoes for 30 days of storage, being a promised technology to preserve cherries tomatoes.

Is Endogenous Ethylene Production Associated with Increased Shoot Number in Gentian Cultured In Vitro? Developing a Method for Ethylene Measurement at Nano Level within a Gas-exchangeable In Vitro System

We investigated the possibility of either exogenous ethylene or endogenous ethylene production having an association with the increase in shoot number when nodal explants of Gentiana spp. ‘Little Pinkie’ were cultured in an in vitro medium supplemented with ethephon (10 mg⋅L–1). For the first time within an in vitro system, we report the application of laser ethylene detector technology, and optimization of the methodology to quantify concentrations of ethylene (in the part-per-billion range) released from ethephon decomposition within the atmosphere of gas-exchangeable culture vessels including nodal explants. Compared with continuous (continuous measurements on the same replicate of vessels) and repeated (sampling same replicate of vessels every 48 hours) sampling methodologies, the nonrepeated (sampling fresh replicate of vessels every 48 hours) method of measurement of ethylene concentration was more representative of the actual condition within vessels. Although no prior published data exist showing the positive or negative effect of gaseous ethylene in the headspace of culture vessels on bud outgrowth in gentian, our study shows gaseous ethylene in the headspace of culture vessels was not effective in increasing shoot formation in gentian explants cultured in vitro, whereas ethephon supplementation in agar was effective. Plant material in culture vessels did not have a significant effect on ethylene production regardless of the presence or absence of ethephon. Therefore, although ethephon supplementation in the medium produced gaseous ethylene in the headspace, it was unlikely to cause endogenous ethylene production in explants, but it did trigger shoot formation in ‘Little Pinkie’, perhaps through decomposition to ethylene within the explant tissue, enhancing the internal ethylene level possibly at a locally high concentration.

Ethephon-Induced Ethylene Enhances Protein Degradation in Source Leaves, but Its High Endogenous Level Inhibits the Development of Regenerative Organs in Brassica napus

To investigate the regulatory role of ethylene in the source-sink relationship for nitrogen remobilization, short-term effects of treatment with different concentrations (0, 25, 50, and 75 ppm) of ethephon (2-chloroethylphosphonic acid, an ethylene inducing agent) for 10 days (EXP 1) and long-term effects at 20 days (Day 30) after treatment with 100 ppm for 10 days (EXP 2) on protein degradation and amino acid transport in foliar sprayed mature leaves of Brassica napus (cv. Mosa) were determined. In EXP 1, endogenous ethylene concentration gradually increased in response to the treated ethephon concentration, leading to the upregulation of senescence-associated gene 12 (SAG12) expression and downregulation of chlorophyll a/b-binding protein (CAB) expression. Further, the increase in ethylene concentration caused a reduction in protein, Rubisco, and amino acid contents in the mature leaves. However, the activity of protease and expression of amino acid transporter (AAP6), an amino acid transport gene, were not significantly affected or slightly suppressed between the treatments with 50 and 75 ppm. In EXP 2, the enhanced ethylene level reduced photosynthetic pigments, leading to an inhibition of flower development without any pod development. A significant increase in protease activity, confirmed using in-gel staining of protease, was also observed in the ethephon-treated mature leaves. Ethephon application enhanced the expression of four amino acid transporter genes (AAP1, AAP2, AAP4, and AAP6) and the phloem loading of amino acids. Significant correlations between ethylene level, induced by ethephon application, and the descriptive parameters of protein degradation and amino acid transport were revealed. These results indicated that an increase in ethylene upregulated nitrogen remobilization in the mature leaves (source), which was accompanied by an increase in proteolytic activity and amino acid transport, but had no benefit to pod (sink) development.

Ethephon-Induced Ethylene Enhances Starch Degradation and Sucrose Transport with an Interactive Abscisic Acid-Mediated Manner in Mature Leaves of Oilseed rape (Brassica napus L.)

The leaf senescence process is characterized by the degradation of macromolecules in mature leaves and the remobilization of degradation products via phloem transport. The phytohormone ethylene mediates leaf senescence. This study aimed to investigate the ethephon-induced ethylene effects on starch degradation and sucrose remobilization through their interactive regulation with other hormones. Ethephon (2-chloroethylphosphonic acid) was used as an ethylene-generating agent. Endogenous hormonal status, carbohydrate compounds, starch degradation-related gene expression, sucrose transporter gene expression, and phloem sucrose loading were compared between the ethephon-treated plants and controls. Foliar ethephon spray enhanced the endogenous ethylene concentration and accelerated leaf senescence, as evidenced by reduced chlorophyll content and enhanced expression of the senescence-related gene SAG12. Ethephon-enhanced ethylene prominently enhanced the endogenous abscisic acid (ABA) level. accompanied with upregulation of ABA synthesis gene 9-cis-epoxycarotenoid dioxygenase (NCED3), ABA receptor gene pyrabactin resistance 1 (PYR1), and ABA signaling genes sucrose non-fermenting 1 (Snf1)-related protein kinase 2 (SnRK2), ABA-responsive element binding 2 (AREB2), and basic-helix-loop-helix (bHLH) transcription factor (MYC2).) Ethephon treatment decreased starch content by enhancing expression of the starch degradation-related genes α-amylase 3 (AMY3) and β-amylase 1 (BAM1), resulting in an increase in sucrose content in phloem exudates with enhanced expression of sucrose transporters, SUT1, SUT4, and SWEET11. These results suggest that a synergistic interaction between ethylene and ABA might account for sucrose accumulation, mainly due to starch degradation in mature leaves and sucrose phloem loading in the ethephon-induced senescent leaves.

ECONOMIC COST-EFFECTIVENES OF ETHYLENE CONSUMPTION IN THE TECHNOLOGICAL PROCESS OF BANANA RIPENING

Banana is a climacteric fruit that undergoes a ripening process in airtight chambers at a certain temperature, appropriate ethylene concentration, and the time it takes for the fruit to reach maturity level 4 on the ripening scale. For commercial purposes an ethylene concentration of 1000 ppm is used, and depending on other factors the concentration may vary. Through a research the best result has been achieved with an ethylene concentration of 900 ppm and the fruit exposure to 18C for a treatment period of 120 h. These parameters reduced costs by RSD 91.896,95 at annual level compared to the commercial concentration of ethylene, the quality of the fruit did not deteriorate greater efficiency in business has been achieved.

Plasma Chemical Method of Decreasing the Ethylene Impurities in the Air

The efficiency of ethylene impurities decomposition in barrierless plasma-chemical system during artificial injection into the air of a sea container has been studied. The experimental study was performed at the air temperature 5oC in container volume 65 m3. The initial level of ozone in the air was 100 ppb. This concentration is below concentration which audible to humans. It has been established that the use of a carbon filter after a plasma chemical system allows to maintain the ozone content in the air at an acceptable level (in terms of human health and food storage). During 24 hours the ethylene concentration increases until an equilibrium concentration is reached. After the plasma chemical rector was switched on, the ethylene concentration in the container began to decrease due to decomposition in the low-temperature plasma and interaction with ozone until new equilibrium concentration was reached. The ozone concentration after plasma chemical reactor was switched to begin increased. After 1 hour, the new minimum equilibrium ethylene and ozone concentration was established. The decrease in concentration occurred exponentially and reached a new equilibrium concentration above zero, which is consistent with the theory. The increase in ozone concentration occurs by about 20-25%. It has been shown that using plasma chemical system based on barrierless plasma chemical reactors can reduce the ethylene concentration by up to 10 times, even for low concentrations of ethylene in the air.

Salicylic Acid from Gandapura Oil as an Inhibitor of Ethylene ‎Biosynthesis in Malus sylvestris L. Mill

Local apple production, especially Anna's apples, continues to decline. One of the Causes of the decline in local apple production is due to the disproportionate use of chemicals, which causes ethylene production in plants to increase. The purpose of this study was to examine the effect of salicylic acid on reducing levels of ethylene concentrations in Anna apple plants. This study used a completely randomized design (CRD) with a single factor, namely the treatment of various salicylic acid concentrations (25 ppm, 50 ppm, 100 ppm, 200 ppm). The results showed that salicylic acid was able to reduce the value of ethylene concentration in plants. Provision of salicylic acid concentrations starting at 25 ppm was able to inhibit ethylene biosynthesis by more than 90%. The best results were shown in samples treated with 200 ppm salicylic acid concentration. By using salicylic acid alternatives, farmers are able to save around Rp3,040,400 in costs in one harvest period. The percentage of costs for salicylic acid in one harvest period is 3.39% of the total production costs, while the cost of the import promoter is 7.92% of the total production costs

Browning of ‘Empire’ and ‘Fuji’ Apples as Affected by Antioxidant Activities

Internal ethylene concentration (IEC) and activities of peroxidase (POX), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) were analyzed to evaluate their effects on browning in late-harvested ‘Empire’ apples (Malus × domestica Borkh.), during air storage at 0.5 °C for five months, and for ‘Fuji’ apples treated with 1-methylcyclopropene (1-MCP), stored for seven months. IEC remained higher in the ‘Empire’ apples compared to values observed in the ‘Fuji’ apples for up to three months in storage, although 1-MCP treatment reduced the IEC in both fruit cultivars. Antioxidant enzymes, such as PPO, POX, and PAL, mostly increased in the flesh and core tissues in both 1-MCP-treated apple cultivars, but were slightly higher in the ‘Empire’ apples between one and three months of storage time. Browning developed in the ‘Empire’ apples after three months of storage, with high susceptibility to incidences of browning observed on the 1-MCP-treated fruit.

Unsteady-State Mathematical Modeling of Hydrocarbon Feedstock Pyrolysis

Hydrocarbon feedstock pyrolysis is an important method for obtaining monomers that are then used to produce various polymer materials. During this process, a mixture of hydrocarbons is heated at a high temperature and in the absence of oxygen. Because of the side reactions of polymerization and polycondensation, coke products are formed and settle on the inner walls of the coil. This decreases the technical efficiency of the hydrocarbon pyrolysis furnace during its operation, making the process unsteady. In the present research, we developed an unsteady-state mathematical model of hydrocarbon feedstock pyrolysis in order to improve the monitoring, forecasting, and optimization of this technological process. This model can calculate the rate of coke deposition along the length of the coil, considering the technological parameters and the composition of the supplied raw materials (the calculated value of coke deposition rate equals 0.01 mm/day). It was shown that with an increase in the propane/butane ratio from 4/1 to 1/4 mol/mol, the ethylene concentration decreases from 3.45 mol/L to 3.35 mol/L.