Enhanced secondary metabolite production and pathway gene expression by leaf explants-induced direct root morphotypes are regulated by combination of growth regulators and culture conditions in Centella asiatica (L.) urban

The basidiomycete Lentinus strigellus was cultivated in three different culture media and the secondary metabolites produced under different culture conditions were isolated and identified. When cultivated in a liquid medium with peptone, L. strigellus afforded the benzopyrans, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6-methoxychromane and (3 R,4 S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. The indole alkaloid echinuline and the anthraquinone fiscione, both unprecedented for the genus Lentinus, were isolated from the mycelium of the fungus. When cultured in Czapek medium enriched with potato broth, the fungus afforded the same benzopyrans except (3 S,4 S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Panepoxydone and isopanepoxydone were also isolated when the microorganism was grown in Czapek medium.

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Influence of plant growth regulators on indirect shoot organogenesis and secondary metabolite production in Aconitum violaceum Jacq.

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.13390 ◽

2013 ◽

Vol 12 (44) ◽

pp. 6287-6293 ◽

Cited By ~ 11

Author(s):

Mishra Rawat Janhvi ◽

Rawat Balwant ◽

Ch Anup ◽

ra ◽

Nautiyal Subhash

Keyword(s):

Plant Growth ◽

Secondary Metabolite ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Shoot Organogenesis ◽

Secondary Metabolite Production ◽

Metabolite Production

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In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

Open Life Sciences ◽

10.2478/s11535-014-0300-7 ◽

2014 ◽

Vol 9 (7) ◽

pp. 686-698 ◽

Cited By ~ 4

Author(s):

Vijay Kumar ◽

Shailesh Singh ◽

Rajib Bandopadhyay ◽

Madan Sharma ◽

Sheela Chandra

Keyword(s):

Heavy Metal ◽

Secondary Metabolites ◽

Secondary Metabolite ◽

Leaf Explants ◽

Secondary Metabolite Production ◽

Direct Organogenesis ◽

Metabolite Production ◽

Swertia Chirayita

AbstractAn efficient protocol of plant regeneration through direct and indirect organogenesis in Swertia chirayita was developed. Explants cultured on Murashige and Skoog medium supplemented with 2,4-D (0.5 mg L−1) with combination of Kinetin (0.5 mg L−1) showed the highest frequency (84%) of callusing and 1.0mg L−1 6-benzyladenine (BA) in combination with (100 mg L−1) Adenine sulphate (Ads) + (0.1 mg L−1) Indole acetic acid (IAA) was excellent for maximum adventitious shoot (12.69 ± 1.30) formation in four week of culture. A maximum number of (7.14 ± 0.99) shoots were developed per leaf explants through direct organogenesis. The highest frequency of rooting (11.46 ± 1.56) was observed on MS medium augmented with IAA (1.0 mg L−1). Well-rooted shoots transferred to plastic pots containing a soilrite: sand mix and then moved to the greenhouse for further growth and development. Four major secondary metabolites were analyzed and quantified using high performance liquid chromatography. Amount of secondary metabolites was found significantly higher, in in vitro plantlets compared to in vivo plantlets and callus raised from S. chirayita. Higher heavy metal accumulation in in vitro as compared to in vivo plantlets correlates higher secondary metabolite production supporting that they play regulatory role in influencing the plant secondary metabolism.

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Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment

PLoS ONE ◽

10.1371/journal.pone.0254804 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0254804

Author(s):

Dwi Kusuma Wahyuni ◽

Shilfiana Rahayu ◽

Andi Hamim Zaidan ◽

Wiwied Ekasari ◽

Sehanat Prasongsuk ◽

...

Keyword(s):

Secondary Metabolite ◽

Methanolic Extract ◽

Leaf Explants ◽

Ethanolic Extract ◽

Antiplasmodial Activity ◽

Secondary Metabolite Production ◽

Gas Chromatography Mass Spectrometry ◽

Metabolite Production ◽

Sonchus Arvensis

Malaria is still a global health problem. Plasmodium is a single-cell protozoan parasite that causes malaria and is transmitted to humans through the female Anopheles mosquito. The previous study showed that Sonchus arvensis L. callus has antiplasmodial activity. Several treatments are needed for callus quality improvement for antimalarial compound production. This study aimed to examine the effect of dolomite [CaMg(CO3)2] on growth (morpho-anatomical structure and biomass), secondary metabolite production, and in vitro antiplasmodial activity of S. arvensis L. callus. In this study, leaf explants were grown in Murashige and Skoog medium with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D, one mg/L) and 6-benzyl amino purine (BAP, 0.5 mg/L) with dolomite (50, 75, 100, 150, and 200 mg/L). The 21 days callus ethanolic and methanolic extract were analyzed by gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). The antiplasmodial test was performed on a blood culture infected with Plasmodium falciparum strain 3D7 using the Rieckmann method. The results showed that dolomite significantly affected callus growth, metabolite profile, and in vitro antiplasmodial activity. Dolomite (150 mg/L) showed the highest biomass (0.590 ± 0.136 g fresh weight and 0.074 ± 0.008 g dry weight). GC-MS analysis detected four compounds from callus ethanolic extract. Pelargonic acid, decanoic acid, and hexadecanoic acid were major compounds. One new terpenoid compound is based on TLC analysis. S. arvensis L. callus has antiplasmodial activity with the IC50 value of 5.037 μg/mL. It was three times lower than leaf methanolic extract and five times lower than leaf ethanolic extract.

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