Effects of estrogen deficiency followed by streptozotocin-induced diabetes on periodontal tissues of female rats

The aim: Find out the nature of the changes sensitivity of the heart to the cholinergic influences the development of diabetes depending on gender. Materials and methods: In experiments on adult male and female rats, type 1 diabetes by momentary introduction of streptozotocin at a dose of 60 mg/ kg into the peritoneal cavity was modeled. Animals were examined after 15, 30, 60 and 90 days after introduction of streptozotocin. We studied the sensitivity of cholinoreceptors of sinus node by the intensity of bradycardia with intravenous injection of acetylcholine and electrical stimulation of the peripheral segment of the right vagus nerve. Results: It was established that the intensity of the negative chronotropic effect of heart of males by electric stimulation of the vagus nerve, which depends on the reserves of acetylcholine in the presynaptic section and sensitivity of postsynaptic cholinoreceptors, increased gradually and after 90 days prevailed the output value in 2.2 times. This occurred at the background of a slight (10 %) decrease in response to exogenous acetylcholine that reflected the decrease in sensitivity of cholinoreceptors. In females the maximum increase (in 4.4 times) of the intensity of the negative chronotropic effect of vagus nerve stimulation was observed after 30 days from the start of the experiment. After 90 days, this indicator prevailed the controlled one in 3.3 times, which occurred at the background of heart response decreasing to exogenous acetylcholine (in 1.7 times). Conclusions: Obtained results suggest a more significant change in state of cholinoceptor system of myocardium of females compared with males in the dynamics of development of streptozotocin-induced diabetes.

Download Full-text

The effects of estrogen deficiency and aging on myocardial deformation and motion in normotensive female rats

Menopause ◽

10.1097/gme.0000000000001884 ◽

2021 ◽

Vol Publish Ahead of Print ◽

Author(s):

Tshiamo T. Maluleke ◽

Aletta M.E. Millen ◽

Frédéric S. Michel

Keyword(s):

Estrogen Deficiency ◽

Myocardial Deformation ◽

Female Rats

Download Full-text

Abstract WP107: Estrogen Deficiency Induces Microbiota Dysbiosis Impacting Stroke Outcome in Female Rats

Stroke ◽

10.1161/str.49.suppl_1.wp107 ◽

2018 ◽

Vol 49 (Suppl_1) ◽

Author(s):

Min Jung Park ◽

Farida Sohrabji

Keyword(s):

Estrogen Deficiency ◽

Female Rats ◽

Stroke Outcome

Download Full-text

Effects of Chronic Ethanol Consumption and Ovariectomy on the Spontaneous Alveolar Bone Loss in Rats

International Journal of Dentistry ◽

10.1155/2020/8873462 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Priscila Cunha Nascimento ◽

Leonardo Oliveira Bittencourt ◽

Soraya O. Pinto ◽

Luana N. S. Santana ◽

Renata Duarte Souza-Rodrigues ◽

...

Keyword(s):

Bone Loss ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Estrogen Deficiency ◽

Female Rats ◽

Chronic Ethanol Consumption ◽

Female Wistar Rats ◽

Postmenopausal Estrogen ◽

Post Hoc ◽

One Way Anova

Postmenopausal estrogen deficiency and ethanol (EtOH) abuse are known risk factors for different diseases including bone tissues. However, little is known about the synergic effects of EtOH abuse and estrogen deficiency on alveolar bone loss in women. The present study evaluated the effects of EtOH chronic exposure and ovariectomy on the alveolar bone loss in female rats. For this, 40 female Wistar rats were randomly divided into 4 groups: control, EtOH exposure, ovariectomy (OVX), and OVX plus EtOH exposure. Initially, half of the animals were ovariectomized at 75 days of age. After that, the groups received distilled water or EtOH 6.5 g/kg/day (20% w/v) for 55 days via gavage. Thereafter, animals were sacrificed and the mandibles were collected, dissected, and separated into hemimandibles. Alveolar bone loss was evaluated by measuring the distance between the cementoenamel junction and the alveolar bone crest through a stereomicroscope in 3 different anatomical regions of the tissue. One-way ANOVA and post hoc Tukey were used to compare groups ( p < 0.05 ). The results showed that the ovariectomy and EtOH exposure per se were able to induce alveolar bone loss, and their association did intensify significantly the effect. Therefore, OVX associated with heavy EtOH exposure increase the spontaneous alveolar bone loss in rats.

Download Full-text

Negligible Effect of Estrogen Deficiency on Development of Skeletal Changes Induced by Type 1 Diabetes in Experimental Rat Models

Mediators of Inflammation ◽

10.1155/2020/2793804 ◽

2020 ◽

Vol 2020 ◽

pp. 1-21

Author(s):

Aleksandra Janas ◽

Ewa Kruczek ◽

Piotr Londzin ◽

Sławomir Borymski ◽

Zenon P. Czuba ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Resorption ◽

Bone Mass ◽

Cancellous Bone ◽

Maximum Load ◽

Estrogen Deficiency ◽

Female Rats ◽

Skeletal System ◽

Tibial Metaphysis ◽

Ovx Rats

Although postmenopausal osteoporosis often occurs concurrently with diabetes, little is known about interactions between estrogen deficiency and hyperglycemia in the skeletal system. In the present study, the effects of estrogen deficiency on the development of biochemical, microstructural, and mechanical changes induced by streptozotocin-induced diabetes mellitus (DM) in the rat skeletal system were investigated. The experiments were carried out on nonovariectomized (NOVX) and ovariectomized (OVX) control and diabetic mature female Wistar rats. Serum levels of bone turnover markers (CTX-I and osteocalcin) and 23 cytokines, bone mass and mineralization, histomorphometric parameters, and mechanical properties of cancellous and compact bone were determined. The results were subjected to two-way ANOVA and principal component analysis (PCA). Estrogen deficiency induced osteoporotic changes, with increased bone resorption and formation, and worsening of microstructure (femoral metaphyseal BV/TV decreased by 13.0%) and mechanical properties of cancellous bone (the maximum load in the proximal tibial metaphysis decreased by 34.2%). DM in both the NOVX and OVX rats decreased bone mass, increased bone resorption and decreased bone formation, and worsened cancellous bone microarchitecture (for example, the femoral metaphyseal BV/TV decreased by 17.3% and 18.1%, respectively, in relation to the NOVX controls) and strength (the maximum load in the proximal tibial metaphysis decreased by 35.4% and 48.1%, respectively, in relation to the NOVX controls). Only in the diabetic rats, profound increases in some cytokine levels were noted. In conclusion, the changes induced by DM in female rats were only slightly intensified by estrogen deficiency. Despite similar effects on bone microstructure and strength, the influence of DM on the skeletal system was based on more profound systemic homeostasis changes than those induced by estrogen deficiency.

Download Full-text

EFFECT OF ENDOCRINE MANIPULATIONS ON GLUCOCORTICOID BINDING CAPACITY IN RAT SKELETAL MUSCLE

Acta Endocrinologica ◽

10.1530/acta.0.0880199 ◽

1978 ◽

Vol 88 (1) ◽

pp. 199-208 ◽

Cited By ~ 15

Author(s):

Michael Mayer ◽

Fred Rosen

Keyword(s):

Skeletal Muscle ◽

Binding Sites ◽

Binding Capacity ◽

Specific Binding ◽

Female Rats ◽

Receptor Protein ◽

Slight Reduction ◽

Binding Assays ◽

Receptor Concentration ◽

Streptozotocin Induced Diabetes

ABSTRACT [3H]Dexamethasone binding capacity in rat muscle cytosol was determined after various endocrine manipulations in an attempt to identify factors which might regulate the level of the cytoplasmic hormone receptor protein. Hypophysectomy and adrenalectomy markedly increased the specific binding of [3H]dexamethasone in skeletal muscle cytosol, while implantation of the MtT tumour which secretes ACTH and growth hormone, as well as treatment with glucocorticoids reduced the glucocorticoid specific binding. Since the effects of hypophysectomy and the MtT tumour depend on the presence of the adrenals, they appear to be mediated via changes in circulating glucocorticoid level. Alloxan- or streptozotocin-induced diabetes caused only a slight reduction in the binding of [3H]dexamethasone in muscle, suggesting that the enhanced responsiveness to glucocorticoids in diabetes is not due to increased glucocorticoid receptor activity. There is a sex-dependent effect on binding, female rats having a higher concentration of binding sites. Furthermore, treatment with the synthetic androgen fluoxymesterone or with glucocorticoids reduces binding, while oestradiol-17β enhances it. The changes in glucocorticoid binding capacity induced by the various endocrine manipulations appear to reflect mainly changes in receptor concentration rather than occupancy, since the binding assays were preformed after a suitable time allowance for removal of the administered hormones by metabolism.

Download Full-text

Modulating Effects of Cholecalciferol Treatment on Estrogen Deficiency-Induced Anxiety-Like Behavior of Adult Female Rats

Folia Medica ◽

10.1515/folmed-2017-0022 ◽

2017 ◽

Vol 59 (2) ◽

pp. 139-158 ◽

Cited By ~ 5

Author(s):

Julia Fedotova ◽

Daria Zarembo ◽

Jozef Dragasek ◽

Martin Caprnda ◽

Peter Kruzliak ◽

...

Keyword(s):

Elevated Plus Maze ◽

Open Field Test ◽

Estrogen Deficiency ◽

Female Rats ◽

Ovx Rats ◽

Plus Maze ◽

17Β Estradiol ◽

Experimental Rat Model ◽

High Doses ◽

Estradiol 17Β

AbstractBackground:Vitamin D can be one of the candidate substances that are used as additional supplementation in the treatment of anxiety-related disorders in women with estrogen imbalance.Materials and methods:The aim of the present study was to examine the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg/day, s.c.) on the anxiety-like behavior and monoamines levels in the rat hippocampus following ovariectomy in female rats. Cholecalciferol was given to ovariectomized (OVX) rats and OVX rats treated with 17β-estradiol (17β-E2, 0.5 μg/rat, s.c.). The anxiety-like behavior was assessed in the elevated plus maze (EPM) and the light-dark tests (LDT), locomotor and grooming activities were assessed in the open-field test (OFT).Results:Cholecalciferol in high doses alone or in combination with 17β-E2-induced anxiolytic-like effects in OVX and OVX rats treated with 17β-E2as evidenced in the EPM and LDT tests, and increased grooming activity in the OFT test. We found that DA and 5-HT levels increased while 5-HT turnover in the hippocampus decreased in these groups of OVX rats.Conclusion:Our results indicate that cholecalciferol in high doses has a marked anxiolytic-like effect due to an increase in the monoamines levels in the experimental rat model of estrogen deficiency.

Download Full-text