Abstract WP107: Estrogen Deficiency Induces Microbiota Dysbiosis Impacting Stroke Outcome in Female Rats

Stroke ◽  
2018 ◽  
Vol 49 (Suppl_1) ◽  
Author(s):  
Min Jung Park ◽  
Farida Sohrabji
Keyword(s):  
Estrogen Deficiency ◽  
Female Rats ◽  
Stroke Outcome

The effects of estrogen deficiency and aging on myocardial deformation and motion in normotensive female rats

Menopause ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Tshiamo T. Maluleke ◽  
Aletta M.E. Millen ◽  
Frédéric S. Michel
Keyword(s):  
Estrogen Deficiency ◽  
Myocardial Deformation ◽  
Female Rats

scholarly journals Effects of Chronic Ethanol Consumption and Ovariectomy on the Spontaneous Alveolar Bone Loss in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Priscila Cunha Nascimento ◽  
Leonardo Oliveira Bittencourt ◽  
Soraya O. Pinto ◽  
Luana N. S. Santana ◽  
Renata Duarte Souza-Rodrigues ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Estrogen Deficiency ◽  
Female Rats ◽  
Chronic Ethanol Consumption ◽  
Female Wistar Rats ◽  
Postmenopausal Estrogen ◽  
Post Hoc ◽  
One Way Anova

Postmenopausal estrogen deficiency and ethanol (EtOH) abuse are known risk factors for different diseases including bone tissues. However, little is known about the synergic effects of EtOH abuse and estrogen deficiency on alveolar bone loss in women. The present study evaluated the effects of EtOH chronic exposure and ovariectomy on the alveolar bone loss in female rats. For this, 40 female Wistar rats were randomly divided into 4 groups: control, EtOH exposure, ovariectomy (OVX), and OVX plus EtOH exposure. Initially, half of the animals were ovariectomized at 75 days of age. After that, the groups received distilled water or EtOH 6.5 g/kg/day (20% w/v) for 55 days via gavage. Thereafter, animals were sacrificed and the mandibles were collected, dissected, and separated into hemimandibles. Alveolar bone loss was evaluated by measuring the distance between the cementoenamel junction and the alveolar bone crest through a stereomicroscope in 3 different anatomical regions of the tissue. One-way ANOVA and post hoc Tukey were used to compare groups ( p < 0.05 ). The results showed that the ovariectomy and EtOH exposure per se were able to induce alveolar bone loss, and their association did intensify significantly the effect. Therefore, OVX associated with heavy EtOH exposure increase the spontaneous alveolar bone loss in rats.

scholarly journals Negligible Effect of Estrogen Deficiency on Development of Skeletal Changes Induced by Type 1 Diabetes in Experimental Rat Models

2020 ◽  
Vol 2020 ◽  
pp. 1-21
Author(s):  
Aleksandra Janas ◽  
Ewa Kruczek ◽  
Piotr Londzin ◽  
Sławomir Borymski ◽  
Zenon P. Czuba ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Resorption ◽  
Bone Mass ◽  
Cancellous Bone ◽  
Maximum Load ◽  
Estrogen Deficiency ◽  
Female Rats ◽  
Skeletal System ◽  
Tibial Metaphysis ◽  
Ovx Rats

Although postmenopausal osteoporosis often occurs concurrently with diabetes, little is known about interactions between estrogen deficiency and hyperglycemia in the skeletal system. In the present study, the effects of estrogen deficiency on the development of biochemical, microstructural, and mechanical changes induced by streptozotocin-induced diabetes mellitus (DM) in the rat skeletal system were investigated. The experiments were carried out on nonovariectomized (NOVX) and ovariectomized (OVX) control and diabetic mature female Wistar rats. Serum levels of bone turnover markers (CTX-I and osteocalcin) and 23 cytokines, bone mass and mineralization, histomorphometric parameters, and mechanical properties of cancellous and compact bone were determined. The results were subjected to two-way ANOVA and principal component analysis (PCA). Estrogen deficiency induced osteoporotic changes, with increased bone resorption and formation, and worsening of microstructure (femoral metaphyseal BV/TV decreased by 13.0%) and mechanical properties of cancellous bone (the maximum load in the proximal tibial metaphysis decreased by 34.2%). DM in both the NOVX and OVX rats decreased bone mass, increased bone resorption and decreased bone formation, and worsened cancellous bone microarchitecture (for example, the femoral metaphyseal BV/TV decreased by 17.3% and 18.1%, respectively, in relation to the NOVX controls) and strength (the maximum load in the proximal tibial metaphysis decreased by 35.4% and 48.1%, respectively, in relation to the NOVX controls). Only in the diabetic rats, profound increases in some cytokine levels were noted. In conclusion, the changes induced by DM in female rats were only slightly intensified by estrogen deficiency. Despite similar effects on bone microstructure and strength, the influence of DM on the skeletal system was based on more profound systemic homeostasis changes than those induced by estrogen deficiency.

scholarly journals Modulating Effects of Cholecalciferol Treatment on Estrogen Deficiency-Induced Anxiety-Like Behavior of Adult Female Rats

Folia Medica ◽  
2017 ◽  
Vol 59 (2) ◽  
pp. 139-158 ◽  
Author(s):  
Julia Fedotova ◽  
Daria Zarembo ◽  
Jozef Dragasek ◽  
Martin Caprnda ◽  
Peter Kruzliak ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Open Field Test ◽  
Estrogen Deficiency ◽  
Female Rats ◽  
Ovx Rats ◽  
Plus Maze ◽  
17Β Estradiol ◽  
Experimental Rat Model ◽  
High Doses ◽  
Estradiol 17Β

AbstractBackground:Vitamin D can be one of the candidate substances that are used as additional supplementation in the treatment of anxiety-related disorders in women with estrogen imbalance.Materials and methods:The aim of the present study was to examine the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg/day, s.c.) on the anxiety-like behavior and monoamines levels in the rat hippocampus following ovariectomy in female rats. Cholecalciferol was given to ovariectomized (OVX) rats and OVX rats treated with 17β-estradiol (17β-E2, 0.5 μg/rat, s.c.). The anxiety-like behavior was assessed in the elevated plus maze (EPM) and the light-dark tests (LDT), locomotor and grooming activities were assessed in the open-field test (OFT).Results:Cholecalciferol in high doses alone or in combination with 17β-E2-induced anxiolytic-like effects in OVX and OVX rats treated with 17β-E2as evidenced in the EPM and LDT tests, and increased grooming activity in the OFT test. We found that DA and 5-HT levels increased while 5-HT turnover in the hippocampus decreased in these groups of OVX rats.Conclusion:Our results indicate that cholecalciferol in high doses has a marked anxiolytic-like effect due to an increase in the monoamines levels in the experimental rat model of estrogen deficiency.

scholarly journals Ovariectomy, but Not Estrogen Deficiency, Increases CYP4A Modulation of  1-Adrenergic Vasoconstriction in Aging Female Rats

2008 ◽  
Vol 21 (6) ◽  
pp. 685-690 ◽  
Author(s):  
D. J. Berezan ◽  
Y. Xu ◽  
J. R. Falck ◽  
A. P. Kundu ◽  
S. T. Davidge
Keyword(s):  
Estrogen Deficiency ◽  
Female Rats

Effects of estrogen deficiency followed by streptozotocin-induced diabetes on periodontal tissues of female rats

2020 ◽  
Vol 51 (4) ◽  
pp. 353-365
Author(s):  
Gisela Rodrigues da Silva Sasso ◽  
Rinaldo Florencio-Silva ◽  
Caio Cesar Navarrete da Fonseca ◽  
Luana Carvalho Cezar ◽  
Adriana Aparecida Ferraz Carbonel ◽  
...  
Keyword(s):  
Estrogen Deficiency ◽  
Female Rats ◽  
Periodontal Tissues ◽  
Streptozotocin Induced Diabetes

Abstract TP277: Diabetes Amplifies Vascular Injury and Worsens Ischemic Stroke Outcome in Young Female Rats: Loss of Protection

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
John P Valenzuela ◽  
Weiguo Li ◽  
Yasir Abdul ◽  
Rebecca Ward ◽  
Sally El-Shafey ◽  
...  
Keyword(s):  
Infarct Size ◽  
Hemorrhagic Transformation ◽  
Young Female ◽  
Female Rats ◽  
Male Rats ◽  
Neurological Deficits ◽  
Control Group ◽  
Stroke Outcome ◽  
Long Term Effects ◽  
Male And Female Rats

Women are protected from stroke until they reach menopause in part due to neuroprotection conferred by sex hormones. We and others have shown that that diabetes increases neurovascular injury and worsens stroke outcomes in males. Given the clinical evidence that diabetes increases stroke risk especially in younger individuals and females, we hypothesized that diabetes worsens stroke outcome even in young females. We further postulated that moderate hyperglycemia worsens hemorrhagic transformation (HT) and outcomes independent of changes in infarct size. High fat diet plus low dose streptozotocin model of diabetes was used. Control and diabetic weight-matched male and female rats (10-12 weeks old, n=5-7) were subjected to embolic stroke with a fibrin-rich humanized clot. Neurological deficits (Bederson score, adhesive removal test -ART and grip strength), infarct size, HT index, and edema ratio were assessed 3 days after surgery (Table). As expected in the control group, females rats had smaller infarct size, less edema, and better functional outcomes as compared to male rats. In the diabetic group, however, HT score was greater and this was more profound in females. Diabetes worsened the functional outcome to a much greater extent in females. While there was partial improvement of neurological deficits by Day 3 in control animals, diabetic animals did not improve but worsened. Additional studies will explore the long-term effects and the underlying mechanisms contributing to worse outcome in young and old diabetic females.

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