Methanol-induced stimulation of growth, intracellular amino acids, and protein content in Chlamydomonas reinhardtii

2014 ◽  
Vol 27 (4) ◽  
pp. 1509-1516 ◽  
Author(s):  
Serhiy S. Stepanov ◽  
Elena K. Zolotareva
Keyword(s):  
Amino Acids ◽  
Chlamydomonas Reinhardtii ◽  
Protein Content ◽  
Intracellular Amino Acids ◽  
Stimulation Of

Stimulation of pancreatic growth by intraduodenal amino acids and HCl

1980 ◽  
Vol 239 (5) ◽  
pp. G400-G405
Author(s):  
L. R. Johnson ◽  
S. J. Dudrick ◽  
P. D. Guthrie
Keyword(s):  
Amino Acids ◽  
Dna Synthesis ◽  
Protein Content ◽  
Infusion Rate ◽  
Exocrine Pancreas ◽  
The Other ◽  
Physiological Conditions ◽  
Pancreatic Growth ◽  
Set Up ◽  
Stimulation Of

Exogenous secretin and cholecystokinin (CCK) have been shown to stimulate the growth of the exocrine pancreas. To conclude that a hormone produces an effect under normal physiological conditions, one must demonstrate that the endogenous hormone also produces the action in question. To release endogenous secretin 0.01 N HCl was continuously infused into the duodenums of eight rats for 5 days. Sixteen animals were prepared with catheters inserted 1 cm below the pylorus. The other eight animals were infused with PO4 buffer, pH 7.5. The infusion rate for both groups was 2 ml/h. Animals were killed at the end of 5 days, and the oxyntic gland mucosa and pancreas were examined. Pancreatic weights, DNA synthesis, DNa, RNA, and protein content were significantly increased in rats receiving acid. An identical experiment was set up to release endogenous CCK. In this experiment eight rats received a solution containing 50 mM phenylalanine and 50 mM tryptophan. The controls received the caloric equivalent in glucose. At the end of 1 wk the pancreases of the rats infused with amino acids averaged 405 (P < 0.001) heavier than the controls. Similar increases occurred in DNA, RNA, and protein content. These studies suggest that endogenous secretin and CCK can be released in amounts sufficient to stimulate growth.

Inter-annual and seasonal dynamics of amino acid, mineral and vitamin composition of silver belly Leiognathus splendens

2014 ◽  
Vol 95 (4) ◽  
pp. 817-828 ◽  
Author(s):  
Kajal Chakraborty ◽  
Deepu Joseph
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
West Coast ◽  
Sensor Data ◽  
The South ◽  
Post Monsoon ◽  
South West ◽  
South West Coast ◽  
Protein Amino Acids

Silver bellies, Leiognathus splendens were studied for their spatial (south-west and south-east coasts of India), annual (2008–2011) and seasonal (pre-monsoon, monsoon and post-monsoon) variations of protein, amino acids, vitamins and minerals. The monthly mean Sea Viewing Wide Field-of-view Sensor data for the period from January 2008 to December 2011 were taken into account to indicate the distribution of the photosynthetic pigment chlorophyll-a to test the hypothesis that surface productivity might be related to nutritional biochemistry of this species. The four year average total protein content and chlorophyll-a showed good correlation during monsoon on the south-west coast and monsoon/post-monsoon on the south-east coast, suggesting that the protein content is prejudiced by the chlorophyll-a concentration. Amino acid scores observed monsoon maxima along the south-west and south-east coasts. Significant seasonal variations in vitamin content were observed at the study locations with high content of vitamins D3, E, K1 and C on the south-west coast. Na content was maximal during pre-monsoon on the south-west coast, while post-monsoon maxima of Ca and K content were observed. The Fe, Mn and Zn were abundant in the samples collected from the south-west coast. The concentration of Se exhibited maximum values post-monsoon along the south-west and south-east coasts. The present study demonstrated L. splendens as a valuable source of the protein, amino acids, minerals and vitamins, showing that this low-value species is a good source of well balanced proteins with high biological value to be qualified as a preferred healthy food for human consumption.

Cloning, characterization, and gene organization of K-Cl cotransporter from pig and human kidney and C. elegans

1998 ◽  
Vol 275 (4) ◽  
pp. F550-F564 ◽  
Author(s):  
Eli J. Holtzman ◽  
Sumit Kumar ◽  
Carol A. Faaland ◽  
Fern Warner ◽  
Paul J. Logue ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Kidney ◽  
Gene Organization ◽  
Kidney Cells ◽  
Hek 293 ◽  
Human Embryonic Kidney Cells ◽  
C Elegans ◽  
Transmembrane Segments ◽  
Extracellular Loops ◽  
Stimulation Of

We isolated and characterized the cDNAs for the human, pig, and Caenorhabditis elegansK-Cl cotransporters. The pig and human homologs are 94% identical and contain 1,085 and 1,086 amino acids, respectively. The deduced protein of the C. elegans K-Cl cotransporter clone (CE-KCC1) contains 1,003 amino acids. The mammalian K-Cl cotransporters share ∼45% similarity with CE-KCC1. Hydropathy analyses of the three clones indicate typical KCC topology patterns with 12 transmembrane segments, large extracellular loops between transmembrane domains 5 and 6 (unique to KCC), and large COOH-terminal domains. Human KCC1 is widely expressed among various tissues. This KCC1 gene spans 23 kb and is organized in 24 exons, whereas the CE-KCC1 gene spans 3.5 kb and contains 10 exons. Transiently and stably transfected human embryonic kidney cells (HEK-293) expressing the human, pig, and C. elegans K-Cl cotransporter fulfilled two (pig) or five (human and C. elegans) criteria for increased expression of the K-Cl cotransporter. The criteria employed were basal K-Cl cotransport; stimulation of cotransport by swelling, N-ethylmaleimide, staurosporine, and reduced cell Mg concentration; and secondary stimulation of Na-K-Cl cotransport.

scholarly journals Reduction of the crude protein content of diets supplemented with essential amino acids for piglets weighing 15 to 30 kilograms

2014 ◽  
Vol 43 (6) ◽  
pp. 301-309 ◽  
Author(s):  
Juliana Beatriz Toledo ◽  
Antonio Claudio Furlan ◽  
Paulo Cesar Pozza ◽  
Jocasta Carraro ◽  
Gabriel Moresco ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Content ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Crude Protein Content
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