Ethanolic extract from Sargassum serratifolium attenuates hyperpigmentation through CREB/ERK signaling pathways in α-MSH-stimulated B16F10 melanoma cells

2017 ◽  
Vol 29 (4) ◽  
pp. 2089-2096 ◽  
Author(s):  
Mohammed Shariful Azam ◽  
Eun-Ji Joung ◽  
Jinkyung Choi ◽  
Hyeung-Rak Kim
Keyword(s):  
Signaling Pathways ◽  
Ethanolic Extract ◽  
Melanoma Cells ◽  
Erk Signaling ◽  
B16f10 Melanoma ◽  
Sargassum Serratifolium ◽  
B16f10 Melanoma Cells

scholarly journals Ethanolic Extract of Hippocampus abdominalis Exerts Anti-Melanogenic Effects in B16F10 Melanoma Cells and Zebrafish Larvae by Activating the ERK Signaling Pathway

Cosmetics ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Ilandarage Menu Neelaka Molagoda ◽  
Yung Hyun Choi ◽  
Seungheon Lee ◽  
Jiwon Sung ◽  
Cho Rong Lee ◽  
...  
Keyword(s):  
Heart Rate ◽  
Signaling Pathway ◽  
Ethanolic Extract ◽  
Adenosine Monophosphate ◽  
Melanoma Cells ◽  
Erk Signaling ◽  
B16f10 Melanoma ◽  
Zebrafish Larvae ◽  
B16f10 Melanoma Cells ◽  
Hippocampus Abdominalis

The big belly seahorse (Hippocampus abdominalis), a well-known ingredient of traditional medicine, possesses anti-inflammatory, anti-aging, anti-fatigue, and anti-thrombotic properties, and also increases male fertility. This study demonstrates that the ethanolic extract of dried H. abdominalis (EEHA) has anti-melanogenic effects in B16F10 melanoma cells and zebrafish larvae. EEHA significantly reduced the α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in B16F10 melanoma cells without causing cytotoxicity. At a concentration of 200 µg/mL, EEHA had significant anti-melanogenic activity in zebrafish larvae, accompanied by a severe reduction in the heart rate (118 ± 17 heartbeats/min) compared to that of the untreated group (185 ± 8 heartbeats/min), indicating that EEHA induces cardiotoxicity at high concentrations. Below 100 µg/mL, EEHA significantly reduced melanogenesis in zebrafish larvae in the presence or absence of α-MSH, while the heart rate remained unaltered. Additionally, EEHA downregulated the release of cyclic adenosine monophosphate (cAMP) and the phosphorylation of cAMP response element-binding protein (CREB) in B16F10 melanoma cells, which inhibited microphthalmia-associated transcription factor (MITF), leading to the inhibition of tyrosinase activity. EEHA also increased the phosphorylation of extracellular-signal regulated kinase (ERK). The ERK inhibitor PD98059 interfered with the anti-melanogenic activity of EEHA in B16F10 melanoma cells and zebrafish larvae, indicating that the ERK signaling pathway might regulate the anti-melanogenic properties of EEHA. Altogether, we conclude that EEHA represses the cAMP–CREB–MITF axis, which consequently inhibits tyrosinase-mediated melanogenesis. We propose that at low concentrations, EEHA can serve as a promising anti-melanogenic agent that could be used to prepare whitening cosmetics and for treating melanogenic disorders.

New Synthesized Galloyl‐RGD Inhibits Melanogenesis by Regulating the CREB and ERK Signaling Pathway in B16F10 Melanoma Cells

2020 ◽  
Vol 96 (6) ◽  
pp. 1321-1331
Author(s):  
Seo Yeon Shin ◽  
Sang Ouk Sun ◽  
Jae Yeon Ko ◽  
Yun Seo Oh ◽  
Seung‐Sik Cho ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
Erk Signaling ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Jeju Magma-Seawater Inhibits α-MSH-Induced Melanogenesis via CaMKKβ-AMPK Signaling Pathways in B16F10 Melanoma Cells

Marine Drugs ◽  
2020 ◽  
Vol 18 (9) ◽  
pp. 473
Author(s):  
Minhyeok Song ◽  
Jihyun Lee ◽  
Young-Joo Kim ◽  
Dang Hieu Hoang ◽  
Wonchae Choe ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Signaling Pathways ◽  
Skin Pigmentation ◽  
Adenosine Monophosphate ◽  
Melanoma Cells ◽  
Mitogen Activated Protein Kinase ◽  
B16f10 Melanoma ◽  
Ampk Signaling ◽  
Calcium Calmodulin Dependent ◽  
B16f10 Melanoma Cells

Melanin protects skin from ultraviolet radiation, toxic drugs, and chemicals. Its synthesis is sophisticatedly regulated by multiple mechanisms, including transcriptional and enzymatic controls. However, uncontrolled excessive production of melanin can cause serious dermatological disorders, such as freckles, melasma, solar lentigo, and cancer. Moreover, melanogenesis disorders are also linked to neurodegenerative diseases. Therefore, there is a huge demand for safer and more potent inhibitors of melanogenesis. In the present study, we report novel inhibitory effects of Jeju magma-seawater (JMS) on melanogenesis induced by α-melanocyte stimulating hormone (α-MSH) in B16F10 melanoma cells. JMS is the abundant underground seawater found in Jeju Island, a volcanic island of Korea. Research into the physiological effects of JMS is rapidly increasing due to its high contents of various minerals that are essential to human health. However, little is known about the effects of JMS on melanogenesis. Here, we demonstrate that JMS safely and effectively inhibits α-MSH-induced melanogenesis via the CaMKKβ (calcium/calmodulin-dependent protein kinase β)-AMPK (5′ adenosine monophosphate-activated protein kinase) signaling pathway. We further demonstrate that AMPK inhibits the signaling pathways of protein kinase A and MAPKs (mitogen-activated protein kinase), which are critical for melanogenesis-related gene expression. Our results highlight the potential of JMS as a novel therapeutic agent for ameliorating skin pigmentation-related disorders.

Inhibitory Effects of Myelophycus simplex Papenfuss Methanol Extract on Melanogenesis in B16F10 Melanoma Cells

2017 ◽  
Vol 46 (1) ◽  
pp. 34-38
Author(s):  
Hyang Suk Kim ◽  
Ji Min Cheon ◽  
Da Hye Kwon ◽  
Eun Ok Choi ◽  
Min Ju Kim ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Melanoma Cells ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

Influence of host defenses on the hepatic colonization of B16F10 melanoma cells

1988 ◽  
Vol 6 (2) ◽  
pp. 153-169 ◽  
Author(s):  
E. Barberá-Guillem ◽  
M. L. Cañavate ◽  
I. Lopez De Tejada ◽  
F. Vidal-Vanaclocha
Keyword(s):  
Melanoma Cells ◽  
Host Defenses ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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