Influence of Exciting-Light Intensity on the Concentration Dependence of Organic Molecules Luminescence in Glassy Matrices*

2019 ◽  
Vol 86 (3) ◽  
pp. 379-383
Author(s):  
M. I. Deryabin ◽  
M. V. Erina ◽  
N. V. Zhdanova
Keyword(s):  
Light Intensity ◽  
Concentration Dependence ◽  
Organic Molecules ◽  
Exciting Light ◽  
Glassy Matrices

scholarly journals FLUORESCENCE SPECTROSCOPIC AND FADING BEHAVIOR OF EHRLICH'S HYPERDIPLOID MOUSE ASCITES TUMOR CELLS SUPRAVITALLY STAINED WITH ACRIDINE ORANGE

1974 ◽  
Vol 22 (7) ◽  
pp. 495-505 ◽  
Author(s):  
JAMES F. GOLDEN ◽  
SEYMOUR S. WEST
Keyword(s):  
Light Intensity ◽  
Acridine Orange ◽  
Tumor Cells ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Exciting Light ◽  
Staining Reaction ◽  
Green Fluorescence ◽  
Fluorescence Emission Spectra ◽  
Mouse Ascites

Corrected fluorescence emission spectra and measurements of fluorescence intensity and fluorescence fading were recorded from Ehrlich's hyperdiploid mouse ascites (EHD) tumor cells supravitally stained with acridine orange (AO). After equilibration of the staining reaction, some EHD tumor cells with dye content greater than 5 x 10–15 mole AO/cell spectroscopically exhibit both red and green fluorescence while the rest fluoresce only green. This behavior contrasts with that previously observed for normal mouse leukocytes. Below 5 x 10–15 mole AO/cell, all EHD tumor cells fluoresce green, but the intensity varies considerably from cell to cell. The fading rate of the green fluorescence appears to be a linear function of fluorescence-exciting light intensity. Absolute light intensity measurements, a necessity for interexperiment comparisons and automation, were based upon a phosphor particle standard. The results presented are indicative of the possibilities for automated cytopathology that can be uncovered by rapid recording, corrected spectrum microspectrofluorophotometry and related biophysical cytochemical techniques.

The distribution of 0-0 energies of organic molecules in glassy matrices

1981 ◽  
Vol 24-25 ◽  
pp. 491-492
Author(s):  
J. Fünfschilling ◽  
I. Zschokke-Gränacher
Keyword(s):  
Organic Molecules ◽  
Glassy Matrices

The determination of the site‐energy distribution of organic molecules dissolved in glassy matrices

1981 ◽  
Vol 75 (8) ◽  
pp. 3669-3673 ◽  
Author(s):  
J. Fünfschilling ◽  
I. Zschokke‐Gränacher ◽  
D. F. Williams
Keyword(s):  
Energy Distribution ◽  
Organic Molecules ◽  
Site Energy ◽  
Glassy Matrices

Effects of temperature, exciting-light intensity, and IR bias lighting on the luminescence of phosphors of the FK series

1970 ◽  
Vol 13 (1) ◽  
pp. 41-44
Author(s):  
K. I. Vorob'ev ◽  
A. S. Gaevskii ◽  
P. A. Korotkov ◽  
A. N. Faidysh
Keyword(s):  
Light Intensity ◽  
Exciting Light ◽  
Effects Of Temperature

High Resolution Replication of Organoclay Surfaces

1982 ◽  
Vol 40 ◽  
pp. 576-577
Author(s):  
W. W. Barker ◽  
W. E. Rigsby ◽  
V. J. Hurst ◽  
W. J. Humphreys
Keyword(s):  
Clay Mineral ◽  
Organic Molecule ◽  
Organic Molecules ◽  
X Ray Diffraction ◽  
Xrd Pattern ◽  
X Ray ◽  
Naturally Occurring ◽  
Silicate Layers ◽  
Mineral Identification

Experimental clay mineral-organic molecule complexes long have been known and some of them have been extensively studied by X-ray diffraction methods. The organic molecules are adsorbed onto the surfaces of the clay minerals, or intercalated between the silicate layers. Natural organo-clays also are widely recognized but generally have not been well characterized. Widely used techniques for clay mineral identification involve treatment of the sample with H2 O2 or other oxidant to destroy any associated organics. This generally simplifies and intensifies the XRD pattern of the clay residue, but helps little with the characterization of the original organoclay. Adequate techniques for the direct observation of synthetic and naturally occurring organoclays are yet to be developed.

Fine structure of the retina of the giant scallop, Placopecten magellanicus

1984 ◽  
Vol 42 ◽  
pp. 312-313
Author(s):  
C.V.L. Powell
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Light Intensity ◽  
Scanning Electron Microscope ◽  
Eye Development ◽  
Preliminary Observation ◽  
Columnar Epithelium ◽  
Placopecten Magellanicus ◽  
Environmental Light ◽  
Scanning Electron

The overall fine structure of the eye in Placopecten is similar to that of other scallops. The optic tentacle consists of an outer columnar epithelium which is modified into a pigmented iris and a cornea (Fig. 1). This capsule encloses the cellular lens, retina, reflecting argentea and the pigmented tapetum. The retina is divided into two parts (Fig. 2). The distal retina functions in the detection of movement and the proximal retina monitors environmental light intensity. The purpose of the present study is to describe the ultrastructure of the retina as a preliminary observation on eye development. This is also the first known presentation of scanning electron microscope studies of the eye of the scallop.

Effect of light intensity on the ultrastructure of the filamentous cyanobacterium, Anabaena variabilis

1988 ◽  
Vol 46 ◽  
pp. 300-301
Author(s):  
C. S. Bricker ◽  
S. R. Barnum ◽  
B. Huang ◽  
J. G. Jaworskl
Keyword(s):  
Fatty Acid ◽  
Light Intensity ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Anabaena Variabilis ◽  
Chloroplast Envelope ◽  
Major Constituent ◽  
Filamentous Cyanobacterium ◽  
Photosynthetic Membrane ◽  
Effect Of Light

Cyanobacteria are Gram negative prokaryotes that are capable of oxygenic photosynthesis. Although there are many similarities between eukaryotes and cyanobacteria in electron transfer and phosphorylation during photosynthesis, there are two features of the photosynthetic apparatus in cyanobacteria which distinguishes them from plants. Cyanobacteria contain phycobiliproteins organized in phycobilisomes on the surface of photosynthetic membrane. Another difference is in the organization of the photosynthetic membranes. Instead of stacked thylakolds within a chloroplast envelope membrane, as seen In eukaryotes, IntracytopIasmlc membranes generally are arranged in three to six concentric layers. Environmental factors such as temperature, nutrition and light fluency can significantly affect the physiology and morphology of cells. The effect of light Intensity shifts on the ultrastructure of Internal membrane in Anabaena variabilis grown under controlled environmental conditions was examined. Since a major constituent of cyanobacterial thylakolds are lipids, the fatty acid content also was measured and correlated with uItrastructural changes. The regulation of fatty acid synthesis in cyanobacteria ultimately can be studied if the fatty acid content can be manipulated.

Direct phase determination in electron crystallography: small organic molecules

1992 ◽  
Vol 50 (2) ◽  
pp. 1166-1167
Author(s):  
Douglas L. Dorset
Keyword(s):  
Organic Molecules ◽  
Data Sets ◽  
Temperature Structure ◽  
3D Analysis ◽  
Intensity Data ◽  
Electron Crystallography ◽  
Phase Determination ◽  
Measured Intensity ◽  
3D Data

The quantitative use of electron diffraction intensity data for the determination of crystal structures represents the pioneering achievement in the electron crystallography of organic molecules, an effort largely begun by B. K. Vainshtein and his co-workers. However, despite numerous representative structure analyses yielding results consistent with X-ray determination, this entire effort was viewed with considerable mistrust by many crystallographers. This was no doubt due to the rather high crystallographic R-factors reported for some structures and, more importantly, the failure to convince many skeptics that the measured intensity data were adequate for ab initio structure determinations.We have recently demonstrated the utility of these data sets for structure analyses by direct phase determination based on the probabilistic estimate of three- and four-phase structure invariant sums. Examples include the structure of diketopiperazine using Vainshtein's 3D data, a similar 3D analysis of the room temperature structure of thiourea, and a zonal determination of the urea structure, the latter also based on data collected by the Moscow group.

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