Mitochondrial metabolic substrate utilization in granulosa cells reflects body mass index and total follicle stimulating hormone dosage in in vitro fertilization patients

The aim of this study was to determine if body mass can be considered a risk factor for infertility, and if we can find any correlations between the age values and the FSH and estradiol values, and between the BMI values and the FSH and estradiol values. Our whole sample contains 100 patients splinted in two groups (pregnant patients N1=57 subjects, 57%, and not � pregnant patients N1=43 subjects,43%). In the first part we conducted our analysis on the whole group and after that we focused the analysis on the two groups and we made some comparisons between the groups. We obtained a medium, extremely significant correlation in all scenarios between the age and the FSH values. This is the best association from all the cases which we tested. In all twelve cases we have a positive correlation (r ] 0). As well, we obtained that a BMI value higher than 25can be considered a risk factor for obtaining a pregnancy (p[0.05, RR ] 1, OR]1) . Our study shows that women who have weight problems have much less chances of conceiving a baby, even if they ovulate normally. The risk of infertility increases proportionally to the extra pounds. Irregular ovulation in women is the most common fertility disorder due to obesity disease.

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The Effect of Maternal Body Mass Index on In Vitro Fertilization-Intracytoplasmic Sperm Injection Treatment: A Prospective Comparative Study

10.21203/rs.3.rs-298772/v1 ◽

2021 ◽

Author(s):

Zeynep Ozturk Inal ◽

Hasan Ali Inal

Keyword(s):

Body Mass Index ◽

In Vitro Fertilization ◽

Body Mass ◽

Intracytoplasmic Sperm Injection ◽

Endometrial Thickness ◽

Maternal Body Mass Index ◽

Maternal Body ◽

Vitro Fertilization ◽

Stimulating Hormone

Abstract Purpose: To evaluate whether or not maternal body mass index (BMI) has an effect on pregnancy rates following in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI) treatment.Methods: A total of 869 patients who had undergone IVF-ICSI treatment between 2012 and 2017 were included in this study. The participants were stratified according to maternal BMI as Group 1 (BMI<25 kg/m2; n=394), Group 2 (25 kg/m2<BMI <30 kg/m2; n=303), and Group 3 (BMI>25 kg/m2; n=172). Basal parameters and IVF-ICSI outcomes were compared between the groups.Results: While there were no differences between the groups in terms of age, smoking status, etiology of infertility, thyroid-stimulating hormone, prolactin levels, antral follicle count, and stimulation protocol (p>0.05), there was significant statistical difference (p<0.05) in terms of BMI, duration of infertility, baseline follicle-stimulating hormone, luteinizing hormone, estradiol (E2), duration of stimulation, total gonadotropin dose required, peak E2 levels, progesterone levels, endometrial thickness on hCG administration, and cycle cancellation rate. In addition, the numbers of MII and 2PN oocytes retrieved and the rates of clinical pregnancy, live births, and miscarriages were also different between the groups (p<0.05).Conclusion: Our data suggest that there is an inverse impact of increased BMI on laboratory and reproductive outcome parameters of IVF-ICSI treatment. Taking cost-effectiveness into consideration, weight loss should be suggested before ovulation is induced.

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Estradiol and progesterone production by cultured granulosa cells cryopreserved from in vitro fertilization patients

Acta Endocrinologica ◽

10.1530/eje.0.1300259 ◽

1994 ◽

Vol 130 (3) ◽

pp. 259-264 ◽

Cited By ~ 5

Author(s):

Patrick M Sluss ◽

Kan-ei Lee ◽

John H Mattox ◽

Patricia C Smith ◽

Margaret C Graham ◽

...

Keyword(s):

In Vitro Fertilization ◽

Granulosa Cells ◽

Follicle Stimulating Hormone ◽

Vitro Fertilization ◽

Cells In Culture ◽

Progesterone Production ◽

Estradiol Secretion ◽

The Impact ◽

Stimulating Hormone

Sluss PM, Lee K, Mattox JH, Smith PC, Graham MC, Partridge AB. Estradiol and progesterone production by cultured granulosa cells cryopreserved from in vitro fertilization patients. Eur J Endocrinol 1994;130:259–64. ISSN 0804–4643 Gonadotropin-stimulated steroidogenesis was studied in cultured human granulosa-lutein cells obtained from patients undergoing procedures for in vitro fertilization. The impact of cryopreservation on cell function in vitro was studied. Granulosa cells obtained from in vitro fertilization patients were cultured in serum-supplemented medium or cryopreserved at −135°C for 2–22 months. Fresh (unfrozen) cells (105) produced estradiol at a rate of 1320 pmol/l (over 72 h) and progesterone at about 2500 nmol/l. Estradiol production by either fresh or cryopreserved granulosa cells in culture was unaffected by physiological concentrations of follicle-stimulating hormone (7IU/l). Adding testosterone (10−7 mol/l) to the medium increased estradiol secretion approximately sixfold. In contrast, progesterone production was not affected by follicle-stimulating hormone or testosterone. No significant differences were observed in cultures of cryopreserved granulosa cells compared to cultures of unfrozen cells with respect to estradiol secretion, the effects of follicle-stimulating hormone or testosterone on estradiol secretion, or progesterone production. Progesterone production by fresh and cryopreserved cells was stimulated by human chorionic gonadotropin. These data indicate that cryopreservation offers the potential to facilitate prospective studies utilizing large numbers of human granulosa-lutein cells in culture. Patrick M Sluss, Reproductive Endocrine Unit, Massachusetts General Hospital East, 149 13th Street, Charlestown, MA 02129, USA

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