Activation of erythropoietin-producing hepatocellular receptor A2 attenuates cell adhesion of human fallopian tube epithelial cells via focal adhesion kinase dephosphorylation

2011 ◽  
Vol 361 (1-2) ◽  
pp. 259-265 ◽  
Author(s):  
Xiao-Yi Yang ◽  
Wei-Jie Zhu ◽  
Huan Jiang
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cells ◽  
Focal Adhesion Kinase ◽  
Fallopian Tube ◽  
Focal Adhesion ◽  
Human Fallopian Tube

scholarly journals Candida albicans Expresses a Focal Adhesion Kinase-Like Protein That Undergoes Increased Tyrosine Phosphorylation upon Yeast Cell Adhesion to Vitronectin and the EA.hy 926 Human Endothelial Cell Line

2002 ◽  
Vol 70 (7) ◽  
pp. 3804-3815 ◽  
Author(s):  
Giorgio Santoni ◽  
Roberta Lucciarini ◽  
Consuelo Amantini ◽  
Jordan Jacobelli ◽  
Elisabetta Spreghini ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Candida Albicans ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Yeast Cell ◽  
Focal Adhesion Kinase ◽  
Cell Line ◽  
Tyrosine Phosphorylation ◽  
Focal Adhesion ◽  
Human Endothelial Cell Line

ABSTRACT The signaling pathways triggered by adherence of Candida albicans to the host cells or extracellular matrix are poorly understood. We provide here evidence in C. albicans yeasts of a p105 focal adhesion kinase (Fak)-like protein (that we termed CaFak), antigenically related to the vertebrate p125Fak, and its involvement in integrin-like-mediated fungus adhesion to vitronectin (VN) and EA.hy 926 human endothelial cell line. Biochemical analysis with different anti-chicken Fak antibodies identified CaFak as a 105-kDa protein and immunofluorescence and cytofluorimetric analysis on permeabilized cells specifically stain C. albicans yeasts; moreover, confocal microscopy evidences CaFak as a cytosolic protein that colocalizes on the membrane with the integrin-like VN receptors upon yeast adhesion to VN. The protein tyrosine kinase (PTK) inhibitors genistein and herbimycin A strongly inhibited C. albicans yeast adhesion to VN and EA.hy 926 endothelial cells. Moreover, engagement of αvβ3 and αvβ5 integrin-like on C. albicans either by specific monoclonal antibodies or upon adhesion to VN or EA.hy 926 endothelial cells stimulates CaFak tyrosine phosphorylation that is blocked by PTK inhibitor. A role for CaFak in C. albicans yeast adhesion was also supported by the failure of VN to stimulate its tyrosine phosphorylation in a C. albicans mutant showing normal levels of CaFak and VNR-like integrins but displaying reduced adhesiveness to VN and EA.hy 926 endothelial cells. Our results suggest that C. albicans Fak-like protein is involved in the control of yeast cell adhesion to VN and endothelial cells.

scholarly journals Cell Adhesion and Focal Adhesion Kinase Regulate Insulin Receptor Substrate-1 Expression

2000 ◽  
Vol 275 (49) ◽  
pp. 38371-38377 ◽  
Author(s):  
Patricia Lebrun ◽  
Véronique Baron ◽  
Christof R. Hauck ◽  
David D. Schlaepfer ◽  
Emmanuel Van Obberghen
Keyword(s):  
Cell Adhesion ◽  
Insulin Receptor ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Insulin Receptor Substrate ◽  
Insulin Receptor Substrate 1

scholarly journals Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

2008 ◽  
Vol 89 (5) ◽  
pp. 1497-1506 ◽  
Author(s):  
Mimi Ghosh ◽  
Todd M. Schaefer ◽  
John V. Fahey ◽  
Jacqueline A. Wright ◽  
Charles R. Wira
Keyword(s):  
Epithelial Cells ◽  
Fallopian Tube ◽  
Poly I:C ◽  
Toll Like Receptor ◽  
Antiviral Responses ◽  
Human Fallopian Tube

HSP27 regulates cell adhesion and invasion via modulation of focal adhesion kinase and MMP-2 expression

2008 ◽  
Vol 87 (6) ◽  
pp. 377-387 ◽  
Author(s):  
Joong-Won Lee ◽  
Hee-Jin Kwak ◽  
Je-Jung Lee ◽  
Yong-Nyun Kim ◽  
Jung Weon Lee ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Adhesion And Invasion

scholarly journals Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model

FEBS Letters ◽  
2010 ◽  
Vol 584 (18) ◽  
pp. 3949-3954 ◽  
Author(s):  
Yanju Ma ◽  
Shingo Semba ◽  
Atsuo Maemoto ◽  
Masayuki Takeuchi ◽  
Isamu Kameshita ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mouse Model ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Colonic Epithelial Cells ◽  
Over Expression

scholarly journals Differential intracellular efficacies of ciprofloxacin and cefixime against Neisseria gonorrhoeae in human fallopian tube organ culture.

1997 ◽  
Vol 41 (7) ◽  
pp. 1547-1551 ◽  
Author(s):  
J P Phanucharas ◽  
G L Gorby
Keyword(s):  
Epithelial Cells ◽  
Confidence Interval ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Cytochalasin D ◽  
Human Fallopian Tube ◽  
Culture Model ◽  
Better Than

This study compared the abilities of ciprofloxacin and cefixime to kill intracellular Neisseria gonorrhoeae in a human fallopian tube organ culture assay. When invasion was inhibited by cytochalasin D, 0.996% of the tissue-associated gonococci survived ciprofloxacin exposure compared to 1.70% of gonococci exposed to cefixime (95% confidence interval for the ratio of the means, 0.267 to 1.30), indicating that the two antibiotics did not significantly differ in the ability to kill extracellular attached organisms. In the absence of cytochalasin D, 1.63% survived ciprofloxacin exposure while 9.76% survived cefixime treatment (95% confidence interval for the ratio of the means, 0.067 to 0.418). These results suggest that ciprofloxacin penetrated epithelial cells and killed intracellular gonococci better than did cefixime. Thus, at concentrations achievable in serum, ciprofloxacin was more effective in total gonococcal killing than cefixime in this human fallopian tube organ culture model.

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